[NHCOLL-L:2488] DNA extraction & IMS

[Julia Sigwart]

Hi folks!

Does anyone have any feedback about extracting DNA from samples 
stored in IMS (industrial
methylated spirit).  IMS is standard in the historical standard in 
Ireland rather than pure ethanol, mainly motivated by a desire to 
avoid paying liquor taxes!  I have had several questions from 
researchers recently who are interested in trying to extract 
molecular sequence data from our older specimens that are entirely 
stored in IMS.  Particularly:
Is there a commercial kit known to be working nicely?
Is there a maximum storage-time beyond which we'd better not even 
bother trying?

Thank you very much in advance!

Cheers,

Julia

-----------------------------------------------
Julia Sigwart

Museum Collections Researcher
National Museum of Ireland &
University College Dublin
http://www.ucd.ie/zoology/sigwart.htm

t. +353 (0)1 660 2040
e. julia.sigwart at ucd.ie
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