[Nhcoll-l] sealing petri dishes for storage

[Christian Baars]

Hi Dee,

Many, many (too many!) years ago I spent a summer looking after the Senckenberg Museum's https://urldefense.proofpoint.com/v2/url?u=http-3A__www.senckenberg.de_&d=CwIFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=McDCvnHFK-XGuw_CKu9yfZMr6akst7lzQJOWEGpGZh0&s=1oO3DMD-_ATozmXStsXBbfNBVlhl2tKTPGwsZEw4EdY&e=  live fresh water algal collections (when they still existed; to my knowledge they don't have them anymore, at least the field station where the collection was kept does not exist anymore). They used to store algae in either in liquid in culture vessels, or on agar plates in petri dishes. Either way, both the vessel openings and the edges of the petri dishes were taped up with Parafilm which is breathable enough to allow for gas exchange but should keep unwanted spores out. I can't see why the same technique shouldn't work well for mould cultures so I would give it a go.

Ironically, we had to check the cultures on a weekly basis for mould infection and, if necessary, pick out individual algal cells to start new, clean cultures.

Best wishes
Christian




Christian Baars PhD AMA

Senior Preventive Conservator  |  National Museum Cardiff  |  Department of Collection Services  |  Cathays Park, Cardiff CF10 3NP, UK
Telephone: +44 (0)29 2057 3302  |  E-mail: christian.baars at museumwales.ac.uk<mailto:christian.baars at museumwales.ac.uk>  |  Twitter: @NMWPrevCons  |  Blog: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.museumwales.ac.uk_blog_-3Fcat-3D2484&d=CwIFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=McDCvnHFK-XGuw_CKu9yfZMr6akst7lzQJOWEGpGZh0&s=IvVpgkiOSGCYxHGZ5n6_NqhUqAV9WDWGzAOggZzbUVI&e= 





From: nhcoll-l-bounces at mailman.yale.edu [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Dee Stubbs-Lee
Sent: 27 June 2016 15:09
To: nhcoll-l at mailman.yale.edu
Subject: [Nhcoll-l] sealing petri dishes for storage

Can anyone recommend a good method for sealing plastic petri dishes containing mold cultures for long term collections storage for our botany department's herbarium? Ideally, the method we are looking for would be secure but reversible, in case we need to directly access the samples in future. Some ideas we have considered include using strips of Parafilm or white plumbers' "tape" around the edges of the petri dishes.

Thanks,
Dee

Dee Stubbs-Lee, CAPC, MA
Conservator / Restauratrice
New Brunswick Museum/
Musée du Nouveau-Brunswick
277 Douglas Avenue
Saint John, New Brunswick
E2K 1E5
Canada
(506)643-2341





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