[Nhcoll-l] Coelacanth deterioration

Perry Poon Perry.Poon at ocean.org
Thu Sep 21 15:42:22 EDT 2017


Hi Simon

There is a fine sandy substrate on the bottom of the Coelacanth display.  I remember that the movers had the Coelacanth in a container filled with alcohol, and the sand and alcohol were added to the glass display tank.  The sand was allowed to settle before the Coelacanth was placed into the display tank.  Some of the gneiss with mica that were floating tin the tank could easily settle on the coelacanth specimen.  This would explain the small amount of glitter that I see on the Coelacanth.

I was thinking of replacing the alcohol and disposing of the sand.

Any thoughts on this?  Is the sand just for cosmetic reasons?

Cheers,
Perry Poon
Coordinator, Props
Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>
D 604 659 3489
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From: Simon Moore [mailto:couteaufin at btinternet.com]
Sent: Thursday, September 14, 2017 1:13 AM
To: Perry Poon <Perry.Poon at ocean.org>; John E Simmons <simmons.johne at gmail.com>; robert Waller <rw at protectheritage.com>
Cc: nhcoll-l at mailman.yale.edu; Dirk Neumann <dirk.neumann at zsm.mwn.de>
Subject: Re: [Nhcoll-l] Coelacanth deterioration

Hi Perry,

Glad (in a way!) that the turbidity test proved to be positive as it indicates that you have a large amount of lipid in solution as well as (probably) coating the specimen and this bears out the reason for the pH lowering.

The glitter is odd. Have there been (or are there still) small pebbles in the display tank?  Sometimes designers will add these to improve the appearance of the specimen and these often contain small bits of glitter, rather like gneiss with mica.

The issue with the 5% formalin versus alcohol - I still stand by what I said although I should have added: provided that it is well maintained! Always a problem these days with thinner collection staffing levels.

With all good wishes, Simon.

Simon Moore MIScT, RSci, FLS, ACR
Conservator of Natural Sciences and Cutlery Historian,
www.natural-history-conservation.com<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=DwIGaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=TUDWCv20FQv6tNK_PFB1LIo9GA5f-gseeLL8JJlnXv8&s=mFSdVO4lox1Rezn3ypU5JOgFzUe-o4tGUHseVPviGDI&e= >



On 14 Sep 2017, at 00:27, Perry Poon <Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>> wrote:

Hi Simon

I did the turbidity test of the coelacanth display alcohol.  I put a sample of the alcohol into a petri dish and the liquid became white and cloudy.

I saw several small droplets of fat in the liquid and a thin  film of fat that covered most of the petri dish on the liquid’s surface.

I was told by a colleague that there are small pieces of glitter on the surface of the Coelacanth’s skin that are less than a millimeter in size.  They are very minute, and I see them when the light hits them at a particular angle.

Have you ever seen these ?  What are these?

Thank you for  all your help and expertise.

Cheers,
Perry Poon
COORDINATOR, PROPS
Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>
D 604 659 3489
________________________________
Our vision is a world in which oceans
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From: Simon Moore [mailto:couteaufin at btinternet.com]
Sent: Tuesday, September 12, 2017 3:27 PM
To: Perry Poon <Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>>
Cc: John E Simmons <simmons.johne at gmail.com<mailto:simmons.johne at gmail.com>>
Subject: Re: [Nhcoll-l] Coelacanth deterioration

Hi Perry,

At pH 4 the skeleton and other calcium salt-containing areas start to decalcify - effectively removing the inorganic matrix.  This leads to scale drop as well as weakening of the skeleton and eventual specimen collapse.

Try a simple turbidity test as well.  A Petri dish on a black background with some water init - pipette some of the alcohol from the specimen - is their any cloudiness or turbidity at all?  If so, the  this would represent saturation of lipid (normally from the liver) and which has likely oxidised forming fatty acids - lowering of pH.  The scales looked rather orange too which would also suggest lipid saturation but that might have been due to photographic lighting?

With all good wishes, Simon.

Simon Moore MIScT, RSci, FLS, ACR
Conservator of Natural Sciences and Cutlery Historian,
www.natural-history-conservation.com<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com_&d=DwIGaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=TUDWCv20FQv6tNK_PFB1LIo9GA5f-gseeLL8JJlnXv8&s=oNHQmwX_Jh1Fr284_gAHuRKWAhrt78CbBURO1kyvqHk&e= >




On 12 Sep 2017, at 20:09, Perry Poon <Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>> wrote:

Hi Simon Moore

The Vancouver Aquarium’s Coelacanth specimen was purchased in the fall of 1971.  It was preserved in Formaldehyde at the time.

The Coelacanth was transferred to alcohol when it was put on display in the Gallery.  There were some alcohol changes done to the display  in the following years.  I don’t have records of the changes

The last complete alcohol change (draining the old alcohol and replacing it with isopropanol) was 2000

Since then,  there has been no change in the isopropanol.

I’ve attached pictures of the Aquarium’s Coelacanth.  You can see the deterioration around the head, fins, and the tail.

The pH reading for the display was done on April 12, 2017.  The litmus paper indicated less than pH 5.  It was pale yellow.

Could you explain to me the hydrogen ion activity at pH 4?  I’m unfamiliar with it, and it may explain the acidity.

There was a density meter reading made on April 12, 2017.  0.9285 g/cm2 (density meter adjusted to alcohol).  The display has 51% Isopropanol (standardize to 20 oC), and 43.4% (non-standardize and not corrected to temperature)

You’ll see in the pictures that there are scales that have fallen from the specimen.

Cheers,
Perry Poon
COORDINATOR, PROPS
Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>
D 604 659 3489
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From: Simon Moore [mailto:couteaufin at btinternet.com]
Sent: Thursday, September 07, 2017 3:17 PM
To: John E Simmons <simmons.johne at gmail.com<mailto:simmons.johne at gmail.com>>
Cc: Perry Poon <Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>>; nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>
Subject: Re: [Nhcoll-l] Coelacanth deterioration

Some history of the specimen’s initial treatment would be most helpful as John has suggested.  pH 4 is the critical level for hydrogen ion activity, so you’re getting close and it obviously isn’t helping the specimen.
Beware of cosmoid scale loss as well as these can be squeezed out over time from their protein-based ‘pockets’ on the skin if these pockets start to shrink.  If the specimen is preserved in alcohol then the scale loss can be repaired using Necolodine solution.  Feel free to come back to me if you need any further advice or more detail and do send pics.

With all good wishes, Simon.

Simon Moore MIScT, RSci, FLS, ACR
Conservator of Natural Sciences and Cutlery Historian,
www.natural-history-conservation.com<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com_&d=DwIGaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=TUDWCv20FQv6tNK_PFB1LIo9GA5f-gseeLL8JJlnXv8&s=oNHQmwX_Jh1Fr284_gAHuRKWAhrt78CbBURO1kyvqHk&e= >





On 7 Sep 2017, at 22:11, John E Simmons <simmons.johne at gmail.com<mailto:simmons.johne at gmail.com>> wrote:

Do you know anything about the preservation history of the specimen? For example, when was it collected; was it frozen before being preserved; was it formaldehyde-fixed or preserved directly in alcohol; has it always been in isopropyl alcohol? Some photographs of the deterioration you have noticed would be very helpful.

--John

John E. Simmons
Museologica
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and
Instructor, Museum Studies
School of Library and Information Science
Kent State University

On Thu, Sep 7, 2017 at 4:26 PM, Perry Poon <Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>> wrote:
I am an employee of the Vancouver Aquarium in British Columbia.  I am looking for advice in the deteriorating nature of the Aquarium’s Coelacanth.  It is currently in 50% isopropanol, and the pH is less than 5.
Deterioration is around the head, fin membranes , and towards the tail.

Looking for expert advice, and I will send pictures to interested parties

Cheers,
Perry Poon
COORDINATOR, PROPS
Perry.Poon at ocean.org<mailto:Perry.Poon at ocean.org>
Vancouver Aquarium
British Columbia
Canada
D 604 659 3489<tel:(604)%20659-3489>
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