[Nhcoll-l] Use of denatured ethanol for short term storage of molecular samples

[Dean Pentcheff]

We have had excellent results on Sanger sequencing (haven't tried genomic)
using Everclear (high-proof drinking alcohol). Specimens were preserved in
Everclear, then transferred to 95% ethanol a few days later. These were
small freshwater crustaceans (aquatic isopods).

I'd be more inclined to go towards drinkable alcohol rather than denatured
alcohol — if it's safe for human consumption, there probably isn't too much
bioactive chemistry going on (other than the ethanol itself).

-Dean
-- 
Dean Pentcheff
pentcheff at gmail.com
dpentche at nhm.org
https://research.nhm.org/disco



On Thu, Apr 18, 2019 at 2:04 PM Nick Cairns <nacairns at gmail.com> wrote:

> Hello everyone,
> I'm seeking guidance on reagents. I'm trying to collect chorus frogs from
> across western Canada (whole and toe clips). These samples will be likely
> be extracted using phenol/chloroform then ethanol (EtOH) precipitation to
> tidy them up. Downstream they'll be used for mtDNA (Sanger) and genomic
> (ddRAD) protocols. The issue is, I am currently in rural Saskatchewan and
> only have denatured EtOH (Fisherbrand Histoprep 95%) available to me.  I
> understand that the additives in some denatured EtOH can cause issues
> downstream but has anyone ever used it for short term storage then replaced
> it later with anhydrous to remove the additives? Can these additives be
> reduced from the tissues after the fact?
> Any insights would be most welcome.
> Thank you,
> Nick
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