[Nhcoll-l] Fluid change for larval fishes

Sarah K. Huber skhuber at vims.edu
Wed Jun 2 12:17:34 EDT 2021


We recently received a donation of larval fishes that are in isopropyl. I'm curious what people think about changing the isopropyl (concentration not noted) to ethanol (70%). Our collection is usually stored in ethanol (including our larval fish), and while I'd prefer everything in ethanol, I'm not sure if the process of changing fluids would compromise these tiny larvae.

The process I use for changing from iso to ethanol is to do 24 hrs in water, then step up (25/50/70) to ethanol in 24 hour increments.

Thanks,
Sarah

Sarah K. Huber, Ph.D.
Curatorial Associate, VIMS Nunnally Ichthyology Collection
Office 804.684.7104 | Collection 804.684.7285
skhuber at vims.edu<mailto:skhuber at vims.edu> | http://www.vims.edu/research/facilities/fishcollection/index.php<https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.vims.edu%2Fresearch%2Ffacilities%2Ffishcollection%2Findex.php&data=04%7C01%7Cskhuber%40vims.edu%7C1063bb0e1daf4ab0024f08d897b1267f%7C8cbcddd9588d4e3b9c1e2367dbdf1740%7C0%7C0%7C637426134417674979%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=OEa%2F3y6PHz5lBFNRFMSFFSdxbyTq%2BBGC5PculkLg7nc%3D&reserved=0>
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