[Nhcoll-l] Preservation issues

Dirk Neumann d.neumann at leibniz-lib.de
Thu Jun 22 10:19:17 EDT 2023


This depends on how well the snake was fixed originally. Re-immersing (and injecting) it in formalin when the cell membranes have already been damages will not improve the condition of the collapsed membranes.

If the initial fixation happened only recently however, it can work (cf. John's comment); additionally, injection (cf. Simon's comment) is recommended (10% instead of 4% for a larger snake).

But as John said, all this depends on the original fixation parameters.

Hope this helps
Dirk


Am 22.06.2023 um 16:02 schrieb Marshall Boyd:
Any advice on procedures if a snake did not fix completely in formalin but has already moved into 20% EtOH. Can I go back to formalin without issues or should I keep moving forward with steady increases to EtOH concentrations?


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--
Stiftung Leibniz-Institut zur Analyse des Biodiversitätswandels
Postanschrift: Adenauerallee 127, 53113 Bonn, Germany

Stiftung des öffentlichen Rechts;
Generaldirektion: Prof. Dr. Bernhard Misof (Generaldirektor), Adrian Grüter (Kaufm. Geschäftsführer)
Sitz der Stiftung: Adenauerallee 160 in Bonn
Vorsitzender des Stiftungsrates: Dr. Michael Wappelhorst
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