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<DIV>Zenker was certainly a standard histological fixative as was Helly and both
contain Pot. dichromate. However their use as gross
(specimen) fixatives is limited - specimens do not last well and even
histol, staining reactions will start to diminish if items are stored in these
fluids.. There is also alcoholic Bouin or Dubosq-Bresil fixative.
However I will add some more to the main list in time.</DIV>
<DIV> </DIV>
<DIV><FONT lang=0 size=2 face=Arial FAMILY="SANSSERIF" PTSIZE="10">With all good
wishes, Simon<BR><BR>Simon Moore MIScT, FLS, ACR,<BR>Conservator of Natural
Sciences and Cutlery Historian,<BR><BR> <A
href="http://www.natural-history-conservation.com/">www.natural-history-conservation.com</A> <BR><A
href="http://www.pocket-fruit-knives.info/">www.pocket-fruit-knives.info</A> <BR><BR><A
href="http://uk.linkedin.com/in/naturalsciencespecimenconserve">http://uk.linkedin.com/in/naturalsciencespecimenconserve</A>
<BR>
<DIV> </DIV>
<DIV>
<DIV>In a message dated 24/10/2012 14:02:13 GMT Daylight Time,
Erik.Ahlander@nrm.se writes:</DIV>
<BLOCKQUOTE
style="BORDER-LEFT: blue 2px solid; PADDING-LEFT: 5px; MARGIN-LEFT: 5px"><FONT
style="BACKGROUND-COLOR: transparent" color=#000000 size=2
face=Arial>Hi,<BR><BR>In the collection in Stockholm (NRM) we have large
material transferred from Stockholm University from the former "Zootomiska
Institutet" engaged in comparing anatomy. Major issue was collecting material
from 1890-1950 for sectioning and staining which leaves us to today with a
large material of animals without heads. Fixation was made with (besides
ethanol and formalin) with Bouin and Smith's solution (I guess its a mixture
of formalin, potassium dichromate, and maybe acetic acid). I think also
Zenker's fixative was sometimes used. Most of these specimens are now
transferred to ethanol, but those fixed with Bouin stain the ethanol yellow
also after many ethanol changes. Smith specimen are uniformely gray with no
pigmentation left.<BR><BR>We also made experiments with storing in Phenoxytol
in the mid 1970s, but the material became soft and we hade to transfer to
ethanol.<BR><BR>Erik �hlander<BR>Collection Manager<BR>Wet Collection,
vertebrates<BR>Swedish Museum of Natural History, Stockholm
(NRM)<BR><BR>-----Original Message-----<BR>From:
nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
On Behalf Of Joachim Haendel<BR>Sent: Wednesday, October 24, 2012 10:59
AM<BR>To: nhcoll-l@mailman.yale.edu<BR>Subject: Re: [Nhcoll-l] Wet collection
best practices - historical fluids<BR><BR>Hello all,<BR><BR>there are some
other fluids used for fixation and preservation.<BR>(some of them are fairly
dangerous):<BR><BR>Solution of Mercury dichloride (Corrosive sublimate)
sometimes used for "vermes" and cnidaria,<BR>also as part of "Krygers
preservation fluid" and "Pennaks fixative"<BR><BR>"Barbers solution" which
contains ethanol, glacial acetic acid and benzene (!)<BR><BR>"Standfuss
preservation fluid": carbon disulfide, benzene and peppermint
oil<BR><BR>and "Bouin solution" which contains also Picric acid (explosive !)
in addition to formalin and lacial acetic
acid.<BR><BR><BR>Joachim<BR><BR><BR>-- <BR>Joachim Haendel
<BR>Centre of Natural History Collections
<BR>of the
Martin-Luther-University<BR>- Entomological Collection -
<BR><BR>Domplatz 4<BR>D-06099 Halle (Saale)<BR>Germany
<BR><BR>Phone: +49 345 - 55 26 447<BR>Fax: +49 345 - 55 27 152<BR>Email:
joachim.haendel@zns.uni-halle.de<BR><BR><BR><BR><BR>_______________________________________________<BR>Nhcoll-l
mailing
list<BR>Nhcoll-l@mailman.yale.edu<BR>http://mailman.yale.edu/mailman/listinfo/nhcoll-l<BR>_______________________________________________<BR>Nhcoll-l
mailing
list<BR>Nhcoll-l@mailman.yale.edu<BR>http://mailman.yale.edu/mailman/listinfo/nhcoll-l</FONT></BLOCKQUOTE></DIV></FONT></DIV></FONT></BODY></HTML>