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</o:shapelayout></xml><![endif]--></head><body lang=EN-GB link=blue vlink=purple><div class=WordSection1><p class=MsoNormal>Hi Sidsel,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I’ve been using DMDM Hydantoin (aka Dekafald) as a preserving medium for some years now, primarily to replace formaldehyde usage with display material. I follow Dries van Dam’s experiences and use a 1:10 dilution of the stock solution. You are still using formaldehyde chemistry but at a very reduced percentage thus its more pleasant to work with and easier to deal with should there be a spill. In my experience the preserving solutions also hold a less acid pH. I’d certainly consider its use over straight formaldehyde in your school service specimens.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Best wishes<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Jules Carter<o:p></o:p></p><p class=MsoNormal>Amgueddfa Cymru – National Museum Wales<span style='font-size:11.0pt;font-family:"Calibri","sans-serif";mso-fareast-language:EN-US'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D;mso-fareast-language:EN-US'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D;mso-fareast-language:EN-US'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0cm 0cm 0cm'><p class=MsoNormal><b><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif"'>From:</span></b><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif"'> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu] <b>On Behalf Of </b>SIMON MOORE<br><b>Sent:</b> 26 February 2014 10:50<br><b>To:</b> Sidsel Marie Willemann; nhcoll-l@mailman.yale.edu<br><b>Subject:</b> Re: [Nhcoll-l] Experience with (which concentration of) DMDM Hydantoin for fixation?<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><div><p class=MsoNormal style='background:white'><span style='color:black'>Hi Sidsel,<o:p></o:p></span></p></div><div><p class=MsoNormal style='background:white'><span style='color:black'>This sounds like a good idea. I am unfortunately inexperienced in the use of Dekafald as a fixative but the Steedman PFP is excellent if used in conjunction with the Steedman fixative which containes formalin but a reduced amount than the straight fixative 10% formalin solution. However please bear in mind that the Steedman solutions were primarily devised for use with zooplankton as opposed to larger and more proteinaceous specimens.<o:p></o:p></span></p></div><div><p class=MsoNormal style='background:white'><span style='color:black'>Hopefully some others with experience of Dekafakd can advise further.<o:p></o:p></span></p></div><div><p class=MsoNormal style='background:white'><span style='color:black'> <o:p></o:p></span></p></div><div><p class=MsoNormal style='background:white'><span style='color:black'>All the best, Simon (Moore)<o:p></o:p></span></p></div><div><p class=MsoNormal style='background:white'><span style='color:black'><o:p> </o:p></span></p></div><div><div><div><p class=MsoNormal style='background:white'><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'>From:</span></b><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'> Sidsel Marie Willemann <<a href="mailto:sidsel.willemann@snm.ku.dk">sidsel.willemann@snm.ku.dk</a>><br><b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a> <br><b>Sent:</b> Tuesday, 25 February 2014, 22:50<br><b>Subject:</b> [Nhcoll-l] Experience with (which concentration of) DMDM Hydantoin for fixation?</span><span style='color:black'><o:p></o:p></span></p></div><div><p class=MsoNormal style='margin-bottom:12.0pt;background:white'><span style='color:black'><br>Dear all,<br><br>For preparing specimens (amphibians and like) for the school service at the museum I work at, I need to use DMDM Hydantoin as a fixative instead of Formaldehyde as one usually does. This is due to health regulations (the children are to touch the specimens).. Does any of you have experience with using DMDM Hydantoin? - what concentration to make? Afterwards the specimens will be preserved in Steedmans PFP solution..<br><br><br>Best regards,<br><br>Sidsel Willemann<br><br>Natural History Museum of Denmark <br><br><br>_______________________________________________<br>Nhcoll-l mailing list<br><a href="mailto:Nhcoll-l@mailman.yale.edu">Nhcoll-l@mailman.yale.edu</a><br><a href="http://mailman.yale.edu/mailman/listinfo/nhcoll-l" target="_blank">http://mailman.yale.edu/mailman/listinfo/nhcoll-l</a><br><br>_______________________________________________ <br>NHCOLL-L is brought to you by the Society for the Preservation of<br>Natural History Collections (SPNHC), an international society whose<br>mission is to improve the preservation, conservation and management of<br>natural history collections to ensure their continuing value to<br>society. See <a href="http://www.spnhc.org/" target="_blank">http://www.spnhc.org/</a> for membership information.<br><br><o:p></o:p></span></p></div></div></div></div></div>
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