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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dear Sonia,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">In general one could say:<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">95% is better for DNA<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">70% is better for morphology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Ethanol is better than IMS<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dries<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black">Andries J. van Dam,
</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">conservator</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black"><br>
<br>
Museum of Anatomy<br>
Leiden University Medical Center <br>
Building 3 (V3-32)<br>
P.O.Box 9600 <br>
2300 RC Leiden <br>
The Netherlands <br>
tel: +31 (0)71 52 68356<br>
E-mail: </span><u><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#0000FE"><a href="mailto:A.J.van_Dam@lumc.nl"><span style="color:blue">A.J.van_Dam@lumc.nl</span></a><br>
</span></u><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black">Visiting address: Hippocratespad 21 </span><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">
</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">Scientific associate</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black">
Natural History Museum London<br>
<a href="http://www.nhm.ac.uk/" target="_blank"><span style="color:blue">http://www.nhm.ac.uk</span></a></span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">Directory Board member</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black"> ICOM-CC<br>
<u><a href="http://www.icom-cc.org/" target="_blank" title="http://www.icom-cc.org/"><span style="color:blue">http://www.icom-cc.org</span></a>
<br>
<br>
</u></span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">Director</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black"> Alcomon Company<br>
<u><a href="http://www.alcomon.com/" target="_blank" title="http://www.alcomon.com/"><span style="color:blue">http://www.alcomon.com</span></a></u></span><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Sonia Rowley<br>
<b>Sent:</b> dinsdag 29 juli 2014 10:32<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [MOGELIJK SPAM ! ********] [Nhcoll-l] 95% vs. ~70% EtOH?<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Dear List<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I am writing with regards any experience and evidence for and against the use of 95% or ~70% EtOH for longterm invertebrate (specifically gorgonian coral) specimen preservation for collections. More and more institutions seem to be turning
to 95% EtOH, however, there are numerous conflicting thoughts and evidence with regards the use of higher concentrations for longterm specimen storage. Furthermore, I understand that there are 2 types of high EtOH concentration; clean and dirty with the latter
being far cheaper but unsuitable for genetic analyses. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Whilst collections are indeed a taxonomic concern, this does include the use of molecular work as part of the suite of tools in determining species differences. The collections I have worked in using ~95% EtOH have had good success with
both storage and genetic work, but these specimens may often be a maximum of 1 - 2 decades old. Conversely, others mention caveats such as the high concentration dehydrating the specimen to such an extent that it disrupts the integrity of both specimen and
DNA. At the same time the often high water content of specimens is said to bring down the EtOH concentration which may also cause issues with specimen degradation. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Please excuse the somewhat rudimentary nature of my question, there does seem a tremendous amount of confliction in what is recommend and therefore it would be really valuable to hear what people have to say and recommend from the list. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Thank you for your time with this matter and look forward to hearing any responses <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Sonia<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">-- <o:p></o:p></p>
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<p class="MsoNormal">Sonia J. Rowley PhD<o:p></o:p></p>
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<p class="MsoNormal">Research Affiliate<o:p></o:p></p>
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<p class="MsoNormal">Bernice Pauahi Bishop Museum<o:p></o:p></p>
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<p class="MsoNormal">1525 Bernice St, Honolulu, <o:p></o:p></p>
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<p class="MsoNormal">HI 96817,<span style="font-family:"franklin-gothic-urw","serif";color:#E8E8E8">,</span>USA<o:p></o:p></p>
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<p class="MsoNormal">+1 808 348 6224<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Research Affiliate<br>
University of Hawai'i at Manoa<br>
2538 McCarthy Mall, Edmondson 312 <o:p></o:p></p>
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<p class="MsoNormal">Honolulu, HI 96822<o:p></o:p></p>
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