<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style id=owaParaStyle>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
        {font-family:"Cambria Math";
        panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
        {font-family:Calibri;
        panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
        {font-family:Tahoma;
        panose-1:2 11 6 4 3 5 4 4 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
        {margin:0in;
        margin-bottom:.0001pt;
        font-size:12.0pt;
        font-family:"Times New Roman","serif";}
a:link, span.MsoHyperlink
        {mso-style-priority:99;
        color:#0563C1;
        text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
        {mso-style-priority:99;
        color:#954F72;
        text-decoration:underline;}
p
        {mso-style-priority:99;
        margin:0in;
        margin-bottom:.0001pt;
        font-size:12.0pt;
        font-family:"Times New Roman","serif";}
span.EmailStyle18
        {mso-style-type:personal-reply;
        font-family:"Calibri","sans-serif";
        color:#1F497D;}
.MsoChpDefault
        {mso-style-type:export-only;
        font-size:10.0pt;}
@page WordSection1
        {size:8.5in 11.0in;
        margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
        {page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="#0563C1" vlink="#954F72">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Sarah<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">It is my understanding that the major reasons for keeping C&S materials in glycerin are support (now that all muscle has been removed from the specimen it is
more susceptible to bending, damage etc.) and clarity (glycerin has a specific refractive index that makes these specimens highly visible). As Glycerin is a vegetable based product and not a preservative or fixative, the thymol is necessary to prevent mold
growth on specimens (one of two crystals is usually enough). Given the difference in specific gravity of glycerin and ethanol I would be concerned about changes in the specimen brought about by a change to 70% ethanol. That being said, theoretically all
that is left is bone and cartilage (and some connective tissue) which should be less susceptible to shrinkage in the first place. I do know that some researchers like to “cut” their glycerin with a little ethanol (10-20%) to make it a little less viscous
and easier to work with but that would have little effect on the specimens. As for specimens that were transferred some time back, I wouldn’t think it would be too harmful to get them back into glycerin as long as this process was staged through increasing
concentrations of glycerin:alcohol.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Andy<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<div>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Dyche Hall<br>
1345 Jayhawk Boulevard<br>
Lawrence, KS, 66045-7561<br>
USA<br>
<br>
Tel: (785) 864-3863<br>
Fax: (785) 864-5335 <br>
Email: <a href="mailto:abentley@ku.edu"><span style="color:blue">abentley@ku.edu</span></a> <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://ichthyology.biodiversity.ku.edu/"><span style="color:blue">http://ichthyology.biodiversity.ku.edu</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">SPNHC President<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.spnhc.org/"><span style="color:blue">http://www.spnhc.org</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"> : : <br>
A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V</span><span lang="EN-AU" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p></o:p></span></p>
</div>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif"">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Sarah K. Huber<br>
<b>Sent:</b> Tuesday, August 19, 2014 10:53 AM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] Long-term Storage of Cleared and Stained Specimens<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div>
<p><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">I am curious to hear what people think is the best long-term storage medium for cleared and stained specimens (in our case fishes). I have seen recommendations for glycerin in concentrations
ranging from 100-70%, and dilutions with water, ethanol, or KOH. I have also seen arguments for and against the addition of thymol. However, since our collection has had mold outbreaks in the past, any long term storage medium we use must have some kind of
additive to prevent molding. <o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"> <o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">In addition, I have come across some older cleared and stained specimens that were transferred to 70% ethanol at some point in the distant past. Is it recommended to keep these
specimens in ethanol or to try and move them back into glycerin?<o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"> <o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">Thanks in advance,<o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">Sarah<o:p></o:p></span></p>
<div>
<div>
<div>
<div>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
</span><span style="font-family:"Tahoma","sans-serif";color:black">Sarah K. Huber, Ph.D.</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
Research Assistant Professor of Biology and Marine Science<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">Collection Manager, VIMS Ichthyology Collection<br>
804.684.7104 | Collection 804.684.7285<br>
skhuber@vims.edu | www.vims.edu<br>
PO Box 1346 | Rt. 1208 Greate Rd., Gloucester Pt., VA 23062<br>
<br>
<span style="border:solid windowtext 1.0pt;padding:0in"><img border="0" width="225" height="62" id="_x0000_i1025" src="cid:image001.jpg@01CFBC58.8DB6B8E0" alt="Image removed by sender. VimsLogo"></span><o:p></o:p></span></p>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</body>
</html>