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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dear Esther,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Just to add to Cathy remarks, formaldehyde polymerizes to paraformaldehyde when formalin is stored below 18 degrees Celsius.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Therefore, I would propose to pack and seal them in vacuum storage bags and place them in crates filled with batting.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">If you contact me off the list, I can provide you with more info about suitable bags and sealing instruments.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dries<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black">Andries J. van Dam,
</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">conservator</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black"><br>
<br>
Museum of Anatomy<br>
Leiden University Medical Center <br>
Building 3 (V3-32)<br>
P.O.Box 9600 <br>
2300 RC Leiden <br>
The Netherlands <br>
tel: +31 (0)71 52 68356<br>
E-mail: </span><u><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#0000FE"><a href="mailto:A.J.van_Dam@lumc.nl">A.J.van_Dam@lumc.nl</a><br>
</span></u><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black">Visiting address: Hippocratespad 21 </span><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">
</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">Scientific associate</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black">
Natural History Museum London<br>
<a href="http://www.nhm.ac.uk/" target="_blank">http://www.nhm.ac.uk</a></span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">Directory Board member</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black"> ICOM-CC<br>
<u><a href="http://www.icom-cc.org/" target="_blank" title="http://www.icom-cc.org/">http://www.icom-cc.org</a>
<br>
<br>
</u></span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:#FF8000">Director</span><span lang="EN-US" style="font-size:8.0pt;font-family:"Verdana","sans-serif";color:black"> Alcomon Company<br>
<u><a href="http://www.alcomon.com/" target="_blank" title="http://www.alcomon.com/">http://www.alcomon.com</a></u></span><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Hawks, Catharine<br>
<b>Sent:</b> dinsdag 2 september 2014 12:25<br>
<b>To:</b> 'Esther Dondorp'; nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [MOGELIJK SPAM ! ******] Re: [Nhcoll-l] Freezing large specimens on formalin<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi Esther<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Unfortunately, formaldehyde polymerizes at low temperatures. This means that the equilibrium necessary for fixation is disrupted by low temperatures.
The free formaldehyde in solution will polymerize, becoming large molecules that will not participate in fixation and this will drive the equilibrium equation in the wrong direction, slowly unfixing your specimens. It is possible that once fully frozen, the
reaction might be slowed, but it would unquestionably still do damage. <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">If specimens are prepared only by denaturation in alcohol (no formaldehyde fixation), low temperatures are a great way to foster preservation.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Cathy<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Catharine Hawks, PA-AIC, FIIC<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Museum Conservator<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">National Museum of Natural History<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">NHB 394 MRC 106<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Smithsonian Institution
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">PO Box 37012<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Washington, DC 20013-7012<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Office 202.633.0835<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Conservation 202.633.4041<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Cell (work) 202.701.8458<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Cell (pers) 703.200.4370<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">
<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a> [<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">mailto:nhcoll-l-bounces@mailman.yale.edu</a>]
<b>On Behalf Of </b>Esther Dondorp<br>
<b>Sent:</b> Tuesday, September 02, 2014 5:29 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] Freezing large specimens on formalin<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">Dear Colleagues,<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">We have a large specimen tank (7*2*2 m) with specimens on formalin. For the construction of our new museum building, we have to get rid of these specimens temporarily until the new building with new specimen tanks is
ready. Because storage in alcohol or formalin somewhere outside the museum will be a challenge, we were thinking of maybe storing these large specimens frozen in a cold storage. And when the new building is ready (somewhere in 2017), to thaw them and to change
them gradually to alcohol.<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">I know freezing is not good for the specimens, but maybe it is different when they have been sitting in formalin for soo long? <o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">I would really appreciate hearing your thoughts on this. <o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">Best wishes,<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">Esther Dondorp<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">Collection manager Reptiles and Amphibians<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US">Naturalis Biodiversity Center<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US"><img border="0" id="_x0000_i1025" src="http://logo.naturalis.nl/logo.png"><o:p></o:p></span></p>
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<p><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial","sans-serif";color:black"><br>
T +31 (0)71 751 73 38, M +31 (0)6 4870 4107<br>
Darwinweg 2, P.O. Box 9517, 2300 RA Leiden, The Netherlands<br>
<a href="mailto:estherdondorp@naturalis.nl" target="_blank"><span style="color:black;text-decoration:none">E estherdondorp@naturalis.nl</span></a>,
<a href="http://www.naturalis.nl" target="_blank"><span style="color:black;text-decoration:none">I www.naturalis.nl</span></a><br>
<br>
Darwinweg 2 - 2333 CR Leiden<br>
E <a href="mailto:Esther.Dondorp@naturalis.nl" target="_blank">Esther.Dondorp@naturalis.nl</a> I , <a href="http://www.naturalis.nl/" target="_blank">www.naturalis.nl</a> </span><span lang="EN-US"><o:p></o:p></span></p>
<p><span lang="EN-US"><o:p> </o:p></span></p>
<p><span lang="EN-US"><o:p> </o:p></span></p>
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