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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">John<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">We too used to store ethanol tissue samples in regular -80 freezers (although all ethanol was drained off to prevent further degrading of DNA by the ethanol)
until we switched to our liquid nitrogen dewar system recently. However at higher temps (-20 freezers) we were instructed that we need to have explosion proof freezers.
</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F4E79;mso-style-textfill-fill-color:#1F4E79;mso-style-textfill-fill-alpha:100.0%">We too were cited
</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F4E79;mso-style-textfill-fill-color:#1F4E79;mso-style-textfill-fill-alpha:100.0%">NFPA 45 10.5.2 and 12.2.2 and Article 501 of code 70 the National Electrical code by our EGS and
fire marshal at the time and as far as I know there is no way around this. It all depends on your local EHS and fire marshal as to whether they pick up on this and enforce it.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F4E79;mso-style-textfill-fill-color:#1F4E79;mso-style-textfill-fill-alpha:100.0%"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F4E79;mso-style-textfill-fill-color:#1F4E79;mso-style-textfill-fill-alpha:100.0%">Andy</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F4E79;mso-style-textfill-fill-color:#1F4E79;mso-style-textfill-fill-alpha:100.0%"><o:p></o:p></span></p>
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Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">Dyche Hall<br>
1345 Jayhawk Boulevard<br>
Lawrence, KS, 66045-7561<br>
USA<br>
<br>
Tel: (785) 864-3863<br>
Fax: (785) 864-5335 <br>
Email: <a href="mailto:abentley@ku.edu">abentley@ku.edu</a> <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=-zLzbwvkpBlNUW26hT9V0sPo63ARzXk_fcfds1MgwqE&s=RzZHZkGvw7OGZ2Wlhqr6CJchdcSg-Af47Do12X6FvNU&e=">http://ichthyology.biodiversity.ku.edu</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">SPNHC President<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org_&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=-zLzbwvkpBlNUW26hT9V0sPo63ARzXk_fcfds1MgwqE&s=2-h3_R4P2oH1jDjhTfM4Vpd3lKd09dTo3-vaXAHb78U&e=">http://www.spnhc.org</a><o:p></o:p></span></p>
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V V V</span><span lang="EN-AU" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>John P. Sullivan<br>
<b>Sent:</b> Friday, January 30, 2015 12:24 PM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] Freezer storage of EtoH tissue samples<o:p></o:p></span></p>
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<p class="MsoNormal">I am curious to know how people on this list deal with cold storage for DNA samples in ethanol.<o:p></o:p></p>
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<p class="MsoNormal">We have thousands of tissue samples and fish fin clips preserved in 95% EtOH in 2ml Sarstedt screw cap vials. We typically store 16 boxes of 81 tubes per freezer rack and can fit 20 racks in a refrigerator/freezer. This makes for a maximum
of 52 liters or 13.7 gallons of EtOH stored in such a refrigerator/freezer.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We have been informed by Environmental Health and Safety at our institution that it is improper to store these in regular freezers (as opposed to prohibitively expensive, specialized “flammable material” freezers).<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">This person cited <span style="font-size:11.5pt;font-family:"Calibri",sans-serif">NFPA 45 10.5.2 and 12.2.2 and Article 501 of code 70 the National Electrical code.</span><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Here are links to EH&S pages at several institutions that seem to indicate ethanol storage requires flammable material freezers:<o:p></o:p></p>
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<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=KF4QbWrSBgmNxBdy9sGpN5ZoayJnK3fFYHaiR9pdSK8&e=">http://www.usd.edu/research/research-and-sponsored-programs/upload/Flammable-Liquids-Storage.pdf</a><o:p></o:p></p>
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<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=qdXV0xQBR7I0tPSImqpMt4pk8mwMg_bQcEQlWWOdHC4&e=">http://www.ehs.washington.edu/fsofire/flamfrig.shtm</a><o:p></o:p></p>
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<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=39ybek4t0u3PswjDfD_nsH9j4KPo9k7Ourm56R-91s8&e=">http://blink.ucsd.edu/safety/research-lab/chemical/liquids/storage/index.html#Determine-allowable-container,-</a><o:p></o:p></p>
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<p class="MsoNormal">However, my informal polling of colleagues on Facebook suggests that many of them do in fact store screw-top tubes with tissue samples in ethanol in regular freezers (though perhaps without the approval/oversight of their EH&S departments).
My view is that fire/explosion risk from storage of these vials must be negligible to non-existent. The flashpoint of pure EtoH is 16.6°C. This temp would only be reached if a freezer failed and no action was taken for a long period of time. The risk of
EtOH vapors concentrating in any quantity near internal mechanisms that could spark are minimal since tubes are sealed.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">My questions are: Has anyone here received explicit clearance from their EH&S to store EtOH tissue samples in regular freezers? If you got this, what was required to obtain it? If you have ethanol-preserved tissue samples, how do you
store them?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">This could make for an interesting and useful conversation, but feel free to contact me off-list if you’re concerned about being “on the record.”<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">- John Sullivan<o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-size:9.0pt;font-family:"Optima",serif;color:black">~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br>
John P. Sullivan, PhD <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Optima",serif;color:black">Collections Archivist & Curatorial Affiliate - Cornell University Museum of Vertebrates<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Optima",serif;color:black">Research Associate - The Academy of Natural Sciences of Drexel University<br>
<br>
Cornell University Museum of Vertebrates <br>
159 Sapsucker Woods Road<br>
Ithaca, New York 14850-1923 USA<br>
<br>
<a href="mailto:jpsullivan@cornell.edu">jpsullivan@cornell.edu</a> / office 607.254.6237, cell 607.342.2234, fax 607.254.2415<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Optima",serif;color:black"><br>
A f r i c h t h y -- The African Ichthyology Research Portal -- <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.africhthy.org&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=vOYiTqQ1fDmmqMF4zkcWu3J1D1KeWFZXZRRhtLF6XNg&e=">http://africhthy.org</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Optima",serif;color:black">Mormyridae LifeDesk — <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mormyrids.lifedesks.org&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=vaCTaJovMJIEav-x6fdGnONaHrdClbK3MW8t1xWshlo&e=">http://mormyrids.lifedesks.org</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Optima",serif;color:black">Campylomormyrus Cam — <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ustream.tv_channel_campylomormyrus-2Dcam&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=hvIH48eTWjNrD653JwXrLOEOfRCJnxfgOue79pRfijo&e=">http://www.ustream.tv/channel/campylomormyrus-cam</a><br>
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<o:p></o:p></span></p>
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