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<div class="moz-cite-prefix">On 1/30/15 10:24 AM, John P. Sullivan
wrote:<br>
</div>
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cite="mid:11CFE39E-338C-4BB7-B4DE-92A3540846A8@cornell.edu"
type="cite">
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<div class="">I am curious to know how people on this list deal
with cold storage for DNA samples in ethanol.</div>
<div class=""><br class="">
</div>
<div class="">We have thousands of tissue samples and fish fin
clips preserved in 95% EtOH in 2ml Sarstedt screw cap vials. We
typically store 16 boxes of 81 tubes per freezer rack and can
fit 20 racks in a refrigerator/freezer. This makes for a
maximum of 52 liters or 13.7 gallons of EtOH stored in such a
refrigerator/freezer.</div>
<div class=""><br class="">
</div>
<div class="">We have been informed by Environmental Health and
Safety at our institution that it is improper to store these in
regular freezers (as opposed to prohibitively expensive,
specialized “flammable material” freezers).</div>
<br>
</blockquote>
<tt>We were given the same instructions from our EHS people, and we
now keep all our cold ethanol in three Fisher Scientific
"Explosion-Proof" freezers, that cost around $2500 each. Money to
cover the extra cost was obtained through various means, different
for each of the three; one was through a <small><small><small><small><small><small><small><small><small><small><small><small><small><small><small><small>
<span style="font-size: 12pt;"><small>"University
of California Genetic
Resources
Conservation Program"</small></span></small></small></small></small></small></small></small></small></small></small></small></small></small></small></small></small></tt><tt>
award. You might find out if your institution has a similar fund
available to you.<br>
<br>
Good luck,<br>
</tt><tt></tt>
<pre class="moz-signature" cols="72">--
Doug Yanega Dept. of Entomology Entomology Research Museum
Univ. of California, Riverside, CA 92521-0314 skype: dyanega
phone: (951) 827-4315 (disclaimer: opinions are mine, not UCR's)
<a class="moz-txt-link-freetext" href="https://urldefense.proofpoint.com/v2/url?u=http-3A__cache.ucr.edu_-7Eheraty_yanega.html&d=AwMD-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=BCd0-M3kbOVOUpSVKK5BIsbwxh934C5k53IfcqDmg5A&s=Hqfj_sVenzJknqavr6j8_x0HyLirI_GVJZaygI6W1HQ&e=">http://cache.ucr.edu/~heraty/yanega.html</a>
"There are some enterprises in which a careful disorderliness
is the true method" - Herman Melville, Moby Dick, Chap. 82</pre>
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