<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 15px; font-family: Calibri, sans-serif;">
<div>
<div>Hi John,</div>
<div><br>
</div>
<div>This may not apply to you, but in Australia both total volume and the volume contained in individual storage containers is used to determine whether an EtOH collection (whether frozen or room temperature) requires specialised housing.</div>
<div><br>
</div>
<div>For example, if you have a total of 52 litres per freezer in a SINGLE container then it would merit requiring a flammable material freezer. However, if your total of 52 L is comprised of thousands of tiny vials then the risk is much lower. This is based
on the premise that IF a fire started in that freezer, then each container would need to be breached for the EtOH to become hazardous. </div>
<div><br>
</div>
<div>We have an EtOH collection at room temperature that equates to many hundreds of litres of EtOH but have been advised that, because each vial is only a maximum of 10mL at most, that this does not require any special provision. Whether we should anyway is
another question… :)</div>
<div><br>
</div>
<div>It might be worth checking if this rule also applies in your State/the US.</div>
<div><br>
</div>
<div>Cheers,</div>
<div>Beth</div>
<div><br>
</div>
<div><!--[if gte mso 9]><xml>
<o:OfficeDocumentSettings>
<o:AllowPNG/>
</o:OfficeDocumentSettings>
</xml><![endif]--><!--[if gte mso 9]><xml>
<w:WordDocument>
<w:View>Normal</w:View>
<w:Zoom>0</w:Zoom>
<w:TrackMoves/>
<w:TrackFormatting/>
<w:PunctuationKerning/>
<w:ValidateAgainstSchemas/>
<w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid>
<w:IgnoreMixedContent>false</w:IgnoreMixedContent>
<w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText>
<w:DoNotPromoteQF/>
<w:LidThemeOther>EN-US</w:LidThemeOther>
<w:LidThemeAsian>JA</w:LidThemeAsian>
<w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript>
<w:Compatibility>
<w:BreakWrappedTables/>
<w:SnapToGridInCell/>
<w:WrapTextWithPunct/>
<w:UseAsianBreakRules/>
<w:DontGrowAutofit/>
<w:SplitPgBreakAndParaMark/>
<w:EnableOpenTypeKerning/>
<w:DontFlipMirrorIndents/>
<w:OverrideTableStyleHps/>
<w:UseFELayout/>
</w:Compatibility>
<m:mathPr>
<m:mathFont m:val="Cambria Math"/>
<m:brkBin m:val="before"/>
<m:brkBinSub m:val="--"/>
<m:smallFrac m:val="off"/>
<m:dispDef/>
<m:lMargin m:val="0"/>
<m:rMargin m:val="0"/>
<m:defJc m:val="centerGroup"/>
<m:wrapIndent m:val="1440"/>
<m:intLim m:val="subSup"/>
<m:naryLim m:val="undOvr"/>
</m:mathPr></w:WordDocument>
</xml><![endif]--><!--[if gte mso 9]><xml>
<w:LatentStyles DefLockedState="false" DefUnhideWhenUsed="true"
DefSemiHidden="true" DefQFormat="false" DefPriority="99"
LatentStyleCount="276">
<w:LsdException Locked="false" Priority="0" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Normal"/>
<w:LsdException Locked="false" Priority="0" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="heading 1"/>
<w:LsdException Locked="false" Priority="0" QFormat="true" Name="heading 2"/>
<w:LsdException Locked="false" Priority="0" QFormat="true" Name="heading 3"/>
<w:LsdException Locked="false" Priority="0" QFormat="true" Name="heading 4"/>
<w:LsdException Locked="false" Priority="0" QFormat="true" Name="heading 5"/>
<w:LsdException Locked="false" Priority="0" QFormat="true" Name="heading 6"/>
<w:LsdException Locked="false" Priority="0" QFormat="true" Name="heading 7"/>
<w:LsdException Locked="false" Priority="0" QFormat="true" Name="heading 8"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 9"/>
<w:LsdException Locked="false" Priority="39" Name="toc 1"/>
<w:LsdException Locked="false" Priority="39" Name="toc 2"/>
<w:LsdException Locked="false" Priority="39" Name="toc 3"/>
<w:LsdException Locked="false" Priority="39" Name="toc 4"/>
<w:LsdException Locked="false" Priority="39" Name="toc 5"/>
<w:LsdException Locked="false" Priority="39" Name="toc 6"/>
<w:LsdException Locked="false" Priority="39" Name="toc 7"/>
<w:LsdException Locked="false" Priority="39" Name="toc 8"/>
<w:LsdException Locked="false" Priority="39" Name="toc 9"/>
<w:LsdException Locked="false" Priority="35" QFormat="true" Name="caption"/>
<w:LsdException Locked="false" Priority="10" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Title"/>
<w:LsdException Locked="false" Priority="1" Name="Default Paragraph Font"/>
<w:LsdException Locked="false" Priority="11" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Subtitle"/>
<w:LsdException Locked="false" Priority="22" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Strong"/>
<w:LsdException Locked="false" Priority="20" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Emphasis"/>
<w:LsdException Locked="false" Priority="59" SemiHidden="false"
UnhideWhenUsed="false" Name="Table Grid"/>
<w:LsdException Locked="false" UnhideWhenUsed="false" Name="Placeholder Text"/>
<w:LsdException Locked="false" Priority="1" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="No Spacing"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 1"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 1"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 1"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 1"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 1"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 1"/>
<w:LsdException Locked="false" UnhideWhenUsed="false" Name="Revision"/>
<w:LsdException Locked="false" Priority="34" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="List Paragraph"/>
<w:LsdException Locked="false" Priority="29" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Quote"/>
<w:LsdException Locked="false" Priority="30" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Intense Quote"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 1"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 1"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 1"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 1"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 1"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 1"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 1"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 1"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 2"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 2"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 2"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 2"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 2"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 2"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 2"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 2"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 2"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 2"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 2"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 2"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 2"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 2"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 3"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 3"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 3"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 3"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 3"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 3"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 3"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 3"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 3"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 3"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 3"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 3"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 3"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 3"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 4"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 4"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 4"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 4"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 4"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 4"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 4"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 4"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 4"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 4"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 4"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 4"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 4"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 4"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 5"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 5"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 5"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 5"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 5"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 5"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 5"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 5"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 5"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 5"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 5"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 5"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 5"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 5"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 6"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 6"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 6"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 6"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 6"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 6"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 6"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 6"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 6"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 6"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 6"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 6"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 6"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 6"/>
<w:LsdException Locked="false" Priority="19" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Subtle Emphasis"/>
<w:LsdException Locked="false" Priority="21" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Intense Emphasis"/>
<w:LsdException Locked="false" Priority="31" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Subtle Reference"/>
<w:LsdException Locked="false" Priority="32" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Intense Reference"/>
<w:LsdException Locked="false" Priority="33" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Book Title"/>
<w:LsdException Locked="false" Priority="37" Name="Bibliography"/>
<w:LsdException Locked="false" Priority="39" QFormat="true" Name="TOC Heading"/>
</w:LatentStyles>
</xml><![endif]--><!--[if gte mso 10]>
<style>
/* Style Definitions */
table.MsoNormalTable
{mso-style-name:"Table Normal";
mso-tstyle-rowband-size:0;
mso-tstyle-colband-size:0;
mso-style-noshow:yes;
mso-style-priority:99;
mso-style-parent:"";
mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
mso-para-margin:0cm;
mso-para-margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:Cambria;
mso-ascii-font-family:Cambria;
mso-ascii-theme-font:minor-latin;
mso-hansi-font-family:Cambria;
mso-hansi-theme-font:minor-latin;
mso-ansi-language:EN-US;}
</style>
<![endif]-->
<div>
<div>
<div style="font-size: medium;"><span class="Apple-style-span" style="font-family: Calibri; font-size: 12px;"><font color="#0099CC"><font face="Arial"><b>_____________________________________</b></font></font></span></div>
<div style="font-size: medium;"><span style="font-size: 12px;"><span class="Apple-style-span" style="font-family: Calibri;"><font color="#0099CC"><font face="Arial"><b>Dr Beth Mantle<br>
</b></font></font></span><font face="Arial">Research Operations Manager, National Research Collections Australia</font></span></div>
</div>
<div style="font-size: medium;"><span style="font-size: 12px;"><font face="Arial">CSIRO National Facilities and Collections</font></span></div>
<div><font face="Calibri,sans-serif" size="2"><font face="Arial">Please note that I work part-time hours: Monday </font>–<font face="Arial"> </font></font><font size="2" style="font-family: Arial;">Wednesday</font><font size="2" style="font-family: Arial;">.</font></div>
<div style="font-size: medium;">
<div><span class="Apple-style-span" style="font-family: Calibri; font-size: 12px;"><font face="Arial"><br>
</font></span></div>
<div><span style="font-size: 12px;"><span class="Apple-style-span" style="font-family: Calibri;"><font face="Arial">P: +61 2 6246 4281<font color="#BED600"><b> | </b></font></font></span><span class="Apple-style-span" style="font-family: Arial;">M: +61 (0)
407 174 427 </span><span class="Apple-style-span" style="color: rgb(190, 214, 0); font-family: Arial;"><b>|</b></span><span class="Apple-style-span" style="font-family: Arial;"> </span><span class="Apple-style-span" style="font-family: Arial;">F: +61 2 6246
5166</span></span></div>
<div><span class="Apple-style-span" style="font-family: Calibri; font-size: 12px;"><font face="Arial"><font color="#0000FF"><a href="applewebdata://C93947AE-4595-4B24-A288-DC5AAC97FFA2/beth.mantle@csiro.au" style="color: purple;">beth.mantle@csiro.au</a></font> <font color="#BED600"><b>|</b></font> <font color="#0000FF"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.csiro.au_&d=AwMF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=ZqR-SjvXJ4Our-p3KyleUlQl3e54ksUPoF-lZa1N0s8&e=" style="color: purple;">www.csiro.au</a> </font> <font color="#BED600"><b>|</b></font> <font color="#0000FF"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.csiro.au_places_anic&d=AwMF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=5l-u08iA2cQK4bVdUD1CjQqdnZtZzv0hD4mGy9jbaA4&e=" style="color: purple;">www.csiro.au</a></font><font color="#0000FF"><br>
</font>Address: <font color="#999999">GPO Box 1600 , Canberra ACT 2601</font></font></span></div>
<div><br>
</div>
</div>
</div>
</div>
</div>
<span id="OLK_SRC_BODY_SECTION">
<div>
<div><font face="Default Sans Serif,Verdana,Arial,Helvetica,sans-serif" size="2"><font color="#990099"><a href="mailto:-----nhcoll-l-bounces@mailman.yale.edu">-----nhcoll-l-bounces@mailman.yale.edu</a> wrote: -----</font>
<div style="padding-left:5px;">
<div style="padding-right:0px;padding-left:5px;border-left:solid black 2px;">To: "<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a>" <<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a>><br>
From: "John P. Sullivan" <js151@cornell.edu><br>
Sent by: <a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a><br>
Date: 01/30/2015 12:26PM<br>
Subject: [Nhcoll-l] Freezer storage of EtoH tissue samples<br>
<br>
<!--Notes ACF
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">-->
<div class="">I am curious to know how people on this list deal with cold storage for DNA samples in ethanol.</div>
<div class=""><br class="">
</div>
<div class="">We have thousands of tissue samples and fish fin clips preserved in 95% EtOH in 2ml Sarstedt screw cap vials. We typically store 16 boxes of 81 tubes per freezer rack and can fit 20 racks in a refrigerator/freezer. This makes for a maximum of
52 liters or 13.7 gallons of EtOH stored in such a refrigerator/freezer.</div>
<div class=""><br class="">
</div>
<div class="">We have been informed by Environmental Health and Safety at our institution that it is improper to store these in regular freezers (as opposed to prohibitively expensive, specialized “flammable material” freezers).</div>
<div class=""><br class="">
</div>
<div class="">This person cited <span style="font-family: Calibri, sans-serif; font-size: 15px;" class="">NFPA 45 10.5.2 and 12.2.2 and Article 501 of code 70 the National Electrical code.</span></div>
<div class=""><br class="">
</div>
<div class="">Here are links to EH&S pages at several institutions that seem to indicate ethanol storage requires flammable material freezers:</div>
<div class=""><br class="">
</div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=KF4QbWrSBgmNxBdy9sGpN5ZoayJnK3fFYHaiR9pdSK8&e=" class="">http://www.usd.edu/research/research-and-sponsored-programs/upload/Flammable-Liquids-Storage.pdf</a></div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=qdXV0xQBR7I0tPSImqpMt4pk8mwMg_bQcEQlWWOdHC4&e=" class="">http://www.ehs.washington.edu/fsofire/flamfrig.shtm</a></div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=39ybek4t0u3PswjDfD_nsH9j4KPo9k7Ourm56R-91s8&e=" class="">http://blink.ucsd.edu/safety/research-lab/chemical/liquids/storage/index.html#Determine-allowable-container,-</a></div>
<div class=""><br class="">
</div>
<div class="">However, my informal polling of colleagues on Facebook suggests that many of them do in fact store screw-top tubes with tissue samples in ethanol in regular freezers (though perhaps without the approval/oversight of their EH&S departments). My
view is that fire/explosion risk from storage of these vials must be negligible to non-existent. The flashpoint of pure EtoH is 16.6°C. This temp would only be reached if a freezer failed and no action was taken for a long period of time. The risk of EtOH
vapors concentrating in any quantity near internal mechanisms that could spark are minimal since tubes are sealed.</div>
<div class=""><br class="">
</div>
<div class="">My questions are: Has anyone here received explicit clearance from their EH&S to store EtOH tissue samples in regular freezers? If you got this, what was required to obtain it? If you have ethanol-preserved tissue samples, how do you store
them?</div>
<div class=""><br class="">
</div>
<div class="">This could make for an interesting and useful conversation, but feel free to contact me off-list if you’re concerned about being “on the record.”</div>
<div class=""><br class="">
</div>
<div class="">- John Sullivan</div>
<div class=""><br class="">
</div>
<div apple-content-edited="true" class="">
<div class="">
<div class=""><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class=""><span class="Apple-style-span">
<div class=""><span class="Apple-style-span">
<div class=""><span class="Apple-style-span">
<div class=""><span class="Apple-style-span">
<div class=""><span class="Apple-style-span">
<div class=""><span class="Apple-style-span">
<div class=""><span class="Apple-style-span">
<div class=""><span class="Apple-style-span">
<div class="">
<div class="">~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br class="">
John P. Sullivan, PhD <br class="">
<br class="">
</div>
<div class="">Collections Archivist & Curatorial Affiliate - Cornell University Museum of Vertebrates</div>
<div class="">Research Associate - The Academy of Natural Sciences of Drexel University<br class="">
<br class="">
Cornell University Museum of Vertebrates <br class="">
159 Sapsucker Woods Road<br class="">
Ithaca, New York 14850-1923 USA<br class="">
<br class="">
<a href="mailto:jpsullivan@cornell.edu" class="">jpsullivan@cornell.edu</a> / office 607.254.6237, cell 607.342.2234, fax 607.254.2415</div>
<div class=""><br class="">
A f r i c h t h y -- The African Ichthyology Research Portal -- <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.africhthy.org&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=vOYiTqQ1fDmmqMF4zkcWu3J1D1KeWFZXZRRhtLF6XNg&e=" style="text-align: -webkit-auto;" class="">http://africhthy.org</a></div>
<div class="">Mormyridae LifeDesk — <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mormyrids.lifedesks.org&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=vaCTaJovMJIEav-x6fdGnONaHrdClbK3MW8t1xWshlo&e=" style="text-align: -webkit-auto;" class="">http://mormyrids.lifedesks.org</a></div>
<div class="">Campylomormyrus Cam — <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ustream.tv_channel_campylomormyrus-2Dcam&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=hvIH48eTWjNrD653JwXrLOEOfRCJnxfgOue79pRfijo&e=" class="">http://www.ustream.tv/channel/campylomormyrus-cam</a><br class="">
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</div>
</div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</div>
</div>
<br class="">
<div><font face="Courier New,Courier,monospace" size="3">_______________________________________________<br>
Nhcoll-l mailing list<br>
<a href="mailto:Nhcoll-l@mailman.yale.edu">Nhcoll-l@mailman.yale.edu</a><br>
<a href="http://mailman.yale.edu/mailman/listinfo/nhcoll-l">http://mailman.yale.edu/mailman/listinfo/nhcoll-l</a><br>
<br>
_______________________________________________ <br>
NHCOLL-L is brought to you by the Society for the Preservation of<br>
Natural History Collections (SPNHC), an international society whose<br>
mission is to improve the preservation, conservation and management of<br>
natural history collections to ensure their continuing value to<br>
society. See <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org&d=AwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5_fiv1_jtiW3r97gMQYIWDUPsSBp67D30HQxQYRH2JU&s=E9fi1y8C-kIVDKRc0pAUFF-5Zv_WGIy2cgRl5HiCpBs&e=">
http://www.spnhc.org</a> for membership information.<br>
</font></div>
</js151@cornell.edu></div>
</div>
<div></div>
</font></div>
</div>
</span>
</body>
</html>