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Re: New website to encourage young entomologists (Katherine Nesheim)
<div class=""><br class="">
</div>
<div class="">Hi Katherine,</div>
<div class=""><br class="">
</div>
<div class="">Have you and Isaac heard about iNaturalist? Sounds like it started up similar to Class Insecta. Now they’re at the California Academy of Sciences and a part of our active citizen science program. There should be lots of new development happening
soon! </div>
<div class=""><br class="">
</div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.inaturalist.org_&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VENhKxCeCEPhyQA3xRpQQgK3vU95OuAGcH7DblDmNsU&s=wk5R74X_63-3MRVCCg1rIk_-UgWSmpPLWjjExvGU0aU&e=" class="">http://www.inaturalist.org/</a><br class="">
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<b class=""><span style="font-size: 9pt; color: rgb(71, 126, 39);" class="">Anne Barber, M.S.<br class="">
</span></b><span style="font-size: 9pt; color: rgb(152, 152, 152);" class="">Digitization Project Manager<br class="">
California Academy of Sciences<br class="">
T 415.379.5177</span></p>
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<blockquote type="cite" class="">
<div class="">On Feb 2, 2015, at 7:15 AM, <a href="mailto:nhcoll-l-request@mailman.yale.edu" class="">
nhcoll-l-request@mailman.yale.edu</a> wrote:</div>
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<div class="">Send Nhcoll-l mailing list submissions to<br class="">
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Today's Topics:<br class="">
<br class="">
1. New website to encourage young entomologists (Katherine Nesheim)<br class="">
2. Re: Freezer storage of EtoH tissue samples (Beth.Mantle@csiro.au)<br class="">
3. Re: Freezer storage of EtoH tissue samples (Dirk Neumann)<br class="">
4. Please post this question (Christopher Kemp)<br class="">
5. Re: Please post this question (Bob Chandler)<br class="">
<br class="">
<br class="">
----------------------------------------------------------------------<br class="">
<br class="">
Message: 1<br class="">
Date: Sun, 1 Feb 2015 13:20:30 -0500<br class="">
From: Katherine Nesheim <kcn23@cornell.edu><br class="">
Subject: [Nhcoll-l] New website to encourage young entomologists<br class="">
To: nhcoll-l@mailman.yale.edu<br class="">
Message-ID:<br class="">
<span class="Apple-tab-span" style="white-space:pre"></span><CAELoYTF8F5BKjbE=wcxE+fEfVf9pqUPcdBDW4ViL9B3v9yyK3g@mail.gmail.com><br class="">
Content-Type: text/plain; charset="utf-8"<br class="">
<br class="">
Hello, Natural History Collections listserv!<br class="">
<br class="">
My name is Katherine Nesheim, and I am a graduate student at Ohio State<br class="">
University. Along with a friend from my undergrad, I have co-founded a<br class="">
website called Class Insecta. This is a free website that lets young<br class="">
scientists upload photos of insects to a digital collection, where the<br class="">
insects are identified by current and former members (including myself and<br class="">
my co-founder) of Cornell University's undergraduate entomology club,<br class="">
Snodgrass and Wigglesworth. Our identifiers do their best to provide an ID<br class="">
and either a short life history or an interesting fact about the insect.<br class="">
Our aim is to provide taxonomic information, while keeping kids interested<br class="">
and informed about their local insect fauna.<br class="">
<br class="">
We are currently seeking funding, mainly through a Kickstarter page (<br class="">
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.kickstarter.com_projects_1145981097_class-2Dinsecta&d=AwIBaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=QIE0vg8wOhhHztMzt-ZHfyJsFzHkEM4TzTiBIby1TNw&s=0dLhxhO3ErOsq1t9NtcXIvv9F9BVZ9ToluevRslyYa8&e=
), to expand<br class="">
classinsecta.org. We'd like to improve the website, which we designed and<br class="">
maintain ourselves, as well as recruit more identifiers and find more kids<br class="">
interested in using Class Insecta.<br class="">
<br class="">
Please check out classinsecta.org as well as our Kickstarter<br class="">
<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.kickstarter.com_projects_1145981097_class-2Dinsecta-3Fref-3Dnav-5Fsearch&d=AwIBaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=QIE0vg8wOhhHztMzt-ZHfyJsFzHkEM4TzTiBIby1TNw&s=c_IuxOxxhdvMiEQp8bzfvWJ2iZ4HaJfjpBsXZQ5-akg&e=
><br class="">
page, and feel free to contact me off-list with any questions or advice.<br class="">
<br class="">
Thank you!<br class="">
<br class="">
Katherine Nesheim<br class="">
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Message: 2<br class="">
Date: Sun, 1 Feb 2015 21:38:22 +0000<br class="">
From: <Beth.Mantle@csiro.au><br class="">
Subject: Re: [Nhcoll-l] Freezer storage of EtoH tissue samples<br class="">
To: <nhcoll-l@mailman.yale.edu><br class="">
Message-ID: <D0F4E6D3.12A0D%Beth.Mantle@csiro.au><br class="">
Content-Type: text/plain; charset="windows-1252"<br class="">
<br class="">
Hi John,<br class="">
<br class="">
This may not apply to you, but in Australia both total volume and the volume contained in individual storage containers is used to determine whether an EtOH collection (whether frozen or room temperature) requires specialised housing.<br class="">
<br class="">
For example, if you have a total of 52 litres per freezer in a SINGLE container then it would merit requiring a flammable material freezer. However, if your total of 52 L is comprised of thousands of tiny vials then the risk is much lower. This is based on
the premise that IF a fire started in that freezer, then each container would need to be breached for the EtOH to become hazardous.<br class="">
<br class="">
We have an EtOH collection at room temperature that equates to many hundreds of litres of EtOH but have been advised that, because each vial is only a maximum of 10mL at most, that this does not require any special provision. Whether we should anyway is another
question? :)<br class="">
<br class="">
It might be worth checking if this rule also applies in your State/the US.<br class="">
<br class="">
Cheers,<br class="">
Beth<br class="">
<br class="">
_____________________________________<br class="">
Dr Beth Mantle<br class="">
Research Operations Manager, National Research Collections Australia<br class="">
CSIRO National Facilities and Collections<br class="">
Please note that I work part-time hours: Monday ? Wednesday.<br class="">
<br class="">
P: +61 2 6246 4281 | M: +61 (0) 407 174 427 | F: +61 2 6246 5166<br class="">
beth.mantle@csiro.au<applewebdata://C93947AE-4595-4B24-A288-DC5AAC97FFA2/beth.mantle@csiro.au> | www.csiro.au<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.csiro.au_&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=ZqR-SjvXJ4Our-p3KyleUlQl3e54ksUPoF-lZa1N0s8&e=
> | www.csiro.au<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.csiro.au_places_anic&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=5l-u08iA2cQK4bVdUD1CjQqdnZtZzv0hD4mGy9jbaA4&e=
><br class="">
Address: GPO Box 1600 , Canberra ACT 2601<br class="">
<br class="">
-----nhcoll-l-bounces@mailman.yale.edu<mailto:-----nhcoll-l-bounces@mailman.yale.edu> wrote: -----<br class="">
To: "nhcoll-l@mailman.yale.edu<mailto:nhcoll-l@mailman.yale.edu>" <nhcoll-l@mailman.yale.edu<mailto:nhcoll-l@mailman.yale.edu>><br class="">
From: "John P. Sullivan"<br class="">
Sent by: nhcoll-l-bounces@mailman.yale.edu<mailto:nhcoll-l-bounces@mailman.yale.edu><br class="">
Date: 01/30/2015 12:26PM<br class="">
Subject: [Nhcoll-l] Freezer storage of EtoH tissue samples<br class="">
<br class="">
I am curious to know how people on this list deal with cold storage for DNA samples in ethanol.<br class="">
<br class="">
We have thousands of tissue samples and fish fin clips preserved in 95% EtOH in 2ml Sarstedt screw cap vials. We typically store 16 boxes of 81 tubes per freezer rack and can fit 20 racks in a refrigerator/freezer. This makes for a maximum of 52 liters or
13.7 gallons of EtOH stored in such a refrigerator/freezer.<br class="">
<br class="">
We have been informed by Environmental Health and Safety at our institution that it is improper to store these in regular freezers (as opposed to prohibitively expensive, specialized ?flammable material? freezers).<br class="">
<br class="">
This person cited NFPA 45 10.5.2 and 12.2.2 and Article 501 of code 70 the National Electrical code.<br class="">
<br class="">
Here are links to EH&S pages at several institutions that seem to indicate ethanol storage requires flammable material freezers:<br class="">
<br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=xsAe-v5Z2ZE0fDchR_s4kuq9QC8BOgRyljnptUCARW4&e=
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=KF4QbWrSBgmNxBdy9sGpN5ZoayJnK3fFYHaiR9pdSK8&e=><br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=5etcgrUTAx-sFGPsHfiGxnvpQbT7G2HHgu8aWeEOzMM&e=
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=qdXV0xQBR7I0tPSImqpMt4pk8mwMg_bQcEQlWWOdHC4&e=><br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=eAeWqJhqyQ0DYcMDFwv9X9ZByrB15yqbTLRlrwbverI&e=
<https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=39ybek4t0u3PswjDfD_nsH9j4KPo9k7Ourm56R-91s8&e=><br class="">
<br class="">
However, my informal polling of colleagues on Facebook suggests that many of them do in fact store screw-top tubes with tissue samples in ethanol in regular freezers (though perhaps without the approval/oversight of their EH&S departments). My view is that
fire/explosion risk from storage of these vials must be negligible to non-existent. The flashpoint of pure EtoH is 16.6?C. This temp would only be reached if a freezer failed and no action was taken for a long period of time. The risk of EtOH vapors concentrating
in any quantity near internal mechanisms that could spark are minimal since tubes are sealed.<br class="">
<br class="">
My questions are: Has anyone here received explicit clearance from their EH&S to store EtOH tissue samples in regular freezers? If you got this, what was required to obtain it? If you have ethanol-preserved tissue samples, how do you store them?<br class="">
<br class="">
This could make for an interesting and useful conversation, but feel free to contact me off-list if you?re concerned about being ?on the record.?<br class="">
<br class="">
- John Sullivan<br class="">
<br class="">
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br class="">
John P. Sullivan, PhD<br class="">
<br class="">
Collections Archivist & Curatorial Affiliate - Cornell University Museum of Vertebrates<br class="">
Research Associate - The Academy of Natural Sciences of Drexel University<br class="">
<br class="">
Cornell University Museum of Vertebrates<br class="">
159 Sapsucker Woods Road<br class="">
Ithaca, New York 14850-1923 USA<br class="">
<br class="">
jpsullivan@cornell.edu<mailto:jpsullivan@cornell.edu> / office 607.254.6237, cell 607.342.2234, fax 607.254.2415<br class="">
<br class="">
A f r i c h t h y -- The African Ichthyology Research Portal -- https://urldefense.proofpoint.com/v2/url?u=http-3A__africhthy.org&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=RpvoRjKnxF9FzBiROwgtiJISVuOjsmNfb24ctARfVlY&e=
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Mormyridae LifeDesk ? https://urldefense.proofpoint.com/v2/url?u=http-3A__mormyrids.lifedesks.org&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=vxMoCKLdJfxvnVZz-3EZgt5Bm2UNiiMrSLGxajprThQ&e=
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mormyrids.lifedesks.org&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=vaCTaJovMJIEav-x6fdGnONaHrdClbK3MW8t1xWshlo&e=><br class="">
Campylomormyrus Cam ? https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ustream.tv_channel_campylomormyrus-2Dcam&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=RTN6COUdHPSpCBODzg8U_GHsT-jJvn4j7ShlxDuJBKo&e=
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ustream.tv_channel_campylomormyrus-2Dcam&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=hvIH48eTWjNrD653JwXrLOEOfRCJnxfgOue79pRfijo&e=><br class="">
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br class="">
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society. See https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=ARtIoMkHtByvjhN12l34ZbIt4Mo2gWmYF1ozsPFYmI8&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org&d=AwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5_fiv1_jtiW3r97gMQYIWDUPsSBp67D30HQxQYRH2JU&s=E9fi1y8C-kIVDKRc0pAUFF-5Zv_WGIy2cgRl5HiCpBs&e=>
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Message: 3<br class="">
Date: Mon, 02 Feb 2015 10:21:23 +0100<br class="">
From: Dirk Neumann <dirk.neumann@zsm.mwn.de><br class="">
Subject: Re: [Nhcoll-l] Freezer storage of EtoH tissue samples<br class="">
To: "John P. Sullivan" <js151@cornell.edu>,<br class="">
<span class="Apple-tab-span" style="white-space:pre"></span>"nhcoll-l@mailman.yale.edu" <nhcoll-l@mailman.yale.edu><br class="">
Message-ID: <54CF4193.4060208@zsm.mwn.de><br class="">
Content-Type: text/plain; charset="windows-1252"<br class="">
<br class="">
We store majority of samples in a -25?C deep freezing chamber, 200 x 2 <br class="">
ml tubes per rack, several ten thousand tubes are currently stored per <br class="">
chamber. Apparently we do not have this strict safety regulations for <br class="">
our -25?C storage; these do apply only for room temperature storage at <br class="">
the moment (where were are allowed to have no more than 20 L of 96 % <br class="">
Ethanol in a fireproof canister inside our alcohol collection) and <br class="">
inside the lab facilities (histology & DNA lab), where we were advised <br class="">
recently that we need to adjust & re-equip fridges and cupboards used <br class="">
for storage of chemicals. The only distinction here is that fluids and <br class="">
especially acids and bases must be stored separately from solids, and <br class="">
that storage of highly reactive peroxides would need special <br class="">
consideration (but we don't use such highly self-reactive peroxides).<br class="">
<br class="">
It might be worth to check the flash point pure ethanol at -25?C; <br class="">
freezing point for 96% is between -73/-110 (?C/F) and -115/-175 (?C/F), <br class="">
so keeping ethanol tubes at -80?C means (depending on dilution of <br class="">
ethanol resulting from dehydrated cells / tissues) keeping tissues close <br class="">
to freezing point of the fluid (but exposing samples to physical damage <br class="">
resulting from formation of ice crystals).<br class="">
<br class="">
Flash point at -25?C should be well below 16?C, and also evaporation <br class="">
pressure inside tubes (and thus building of highly combustible <br class="">
ethanol-air mixtures) is considerably lower. It might perhaps be worth <br class="">
checking this with colleagues from Chemistry departments at University. <br class="">
I doubt that a spark released at the inside of a freezer (which could <br class="">
either be released from the lamp or the control) could ignite the <br class="">
content of a tube. More likely in case of failure (of the compressor) <br class="">
would be that the insulation catches fire. Perhaps a physical test <br class="">
witnessed by the fire marshal helps: try to ignite a 2 ml ethanol tube <br class="">
at room temperature under controlled conditions, place a box with more <br class="">
ethanol tubes in close vicinity and see what happens. I seriously doubt <br class="">
that these would catch fire, too. But the devil is a squirrel and this <br class="">
is not a question about good judgement but rather an administrative one <br class="">
which helps to demonstrate power ...<br class="">
<br class="">
<br class="">
Hope this helps,<br class="">
all the best<br class="">
Dirk<br class="">
<br class="">
<br class="">
Am 30.01.2015 um 19:24 schrieb John P. Sullivan:<br class="">
<blockquote type="cite" class="">I am curious to know how people on this list deal with cold storage
<br class="">
for DNA samples in ethanol.<br class="">
<br class="">
We have thousands of tissue samples and fish fin clips preserved in <br class="">
95% EtOH in 2ml Sarstedt screw cap vials. We typically store 16 boxes <br class="">
of 81 tubes per freezer rack and can fit 20 racks in a <br class="">
refrigerator/freezer. This makes for a maximum of 52 liters or 13.7 <br class="">
gallons of EtOH stored in such a refrigerator/freezer.<br class="">
<br class="">
We have been informed by Environmental Health and Safety at our <br class="">
institution that it is improper to store these in regular freezers (as <br class="">
opposed to prohibitively expensive, specialized ?flammable material? <br class="">
freezers).<br class="">
<br class="">
This person cited NFPA 45 10.5.2 and 12.2.2 and Article 501 of code <br class="">
70 the National Electrical code.<br class="">
<br class="">
Here are links to EH&S pages at several institutions that seem to <br class="">
indicate ethanol storage requires flammable material freezers:<br class="">
<br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=hcca_EI9_YzWlS358en6nvshcyH3f5JbaP_c1wUZc_o&e=
<br class="">
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=KF4QbWrSBgmNxBdy9sGpN5ZoayJnK3fFYHaiR9pdSK8&e=><br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=fA8WgYbrXo2a0xhrAbuH9TgNFIEuZE2K3wkcOt-fvQs&e=
<br class="">
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=qdXV0xQBR7I0tPSImqpMt4pk8mwMg_bQcEQlWWOdHC4&e=><br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=daWvHB4CEj8Wv0AXh0Dy0s-GlJVrXTSUqyFAxAcPPsA&e=
<br class="">
<https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=39ybek4t0u3PswjDfD_nsH9j4KPo9k7Ourm56R-91s8&e=><br class="">
<br class="">
However, my informal polling of colleagues on Facebook suggests that <br class="">
many of them do in fact store screw-top tubes with tissue samples in <br class="">
ethanol in regular freezers (though perhaps without the <br class="">
approval/oversight of their EH&S departments). My view is that <br class="">
fire/explosion risk from storage of these vials must be negligible to <br class="">
non-existent. The flashpoint of pure EtoH is 16.6?C. This temp would <br class="">
only be reached if a freezer failed and no action was taken for a long <br class="">
period of time. The risk of EtOH vapors concentrating in any quantity <br class="">
near internal mechanisms that could spark are minimal since tubes are <br class="">
sealed.<br class="">
<br class="">
My questions are: Has anyone here received explicit clearance from <br class="">
their EH&S to store EtOH tissue samples in regular freezers? If you <br class="">
got this, what was required to obtain it? If you have <br class="">
ethanol-preserved tissue samples, how do you store them?<br class="">
<br class="">
This could make for an interesting and useful conversation, but feel <br class="">
free to contact me off-list if you?re concerned about being ?on the <br class="">
record.?<br class="">
<br class="">
- John Sullivan<br class="">
<br class="">
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br class="">
John P. Sullivan, PhD<br class="">
<br class="">
Collections Archivist & Curatorial Affiliate - Cornell University <br class="">
Museum of Vertebrates<br class="">
Research Associate - The Academy of Natural Sciences of Drexel University<br class="">
<br class="">
Cornell University Museum of Vertebrates<br class="">
159 Sapsucker Woods Road<br class="">
Ithaca, New York 14850-1923 USA<br class="">
<br class="">
jpsullivan@cornell.edu <mailto:jpsullivan@cornell.edu> / office <br class="">
607.254.6237, cell 607.342.2234, fax 607.254.2415<br class="">
<br class="">
A f r i c h t h y -- The African Ichthyology Research Portal -- <br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__africhthy.org&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=uMZgSAMzcEy96E59RMY1C4ntzJM7O7-sC2F1tDNBQGo&e= <br class="">
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.africhthy.org&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=vOYiTqQ1fDmmqMF4zkcWu3J1D1KeWFZXZRRhtLF6XNg&e=><br class="">
Mormyridae LifeDesk ? https://urldefense.proofpoint.com/v2/url?u=http-3A__mormyrids.lifedesks.org&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=4uoK-Knd6n2S0Pjdvp5L-Ov0MEZVk64ZUrqruXN-RDs&e=
<br class="">
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mormyrids.lifedesks.org&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=vaCTaJovMJIEav-x6fdGnONaHrdClbK3MW8t1xWshlo&e=><br class="">
Campylomormyrus Cam ? <br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ustream.tv_channel_campylomormyrus-2Dcam&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=g0jKtby3TucSI_fcVkRQ1DVhoGQsoeMk0jVrl_uolPc&e=
<br class="">
<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ustream.tv_channel_campylomormyrus-2Dcam&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=hvIH48eTWjNrD653JwXrLOEOfRCJnxfgOue79pRfijo&e=><br class="">
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br class="">
<br class="">
<br class="">
<br class="">
_______________________________________________<br class="">
Nhcoll-l mailing list<br class="">
Nhcoll-l@mailman.yale.edu<br class="">
http://mailman.yale.edu/mailman/listinfo/nhcoll-l<br class="">
<br class="">
_______________________________________________<br class="">
NHCOLL-L is brought to you by the Society for the Preservation of<br class="">
Natural History Collections (SPNHC), an international society whose<br class="">
mission is to improve the preservation, conservation and management of<br class="">
natural history collections to ensure their continuing value to<br class="">
society. See https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=Mf3h4RxBtaKfuFJ9GfYZVCl_TStHbqNvg6OStSpaehM&e= for
membership information.<br class="">
</blockquote>
<br class="">
<br class="">
-- <br class="">
Dirk Neumann<br class="">
<br class="">
Tel: 089 / 8107-111<br class="">
Fax: 089 / 8107-300<br class="">
email: Dirk.Neumann(a)zsm.mwn.de<br class="">
<br class="">
Postanschrift:<br class="">
<br class="">
Staatliche Naturwissenschaftliche Sammlungen Bayerns<br class="">
Zoologische Staatssammlung M?nchen<br class="">
Dirk Neumann, Sektion Ichthyologie / DNA-Labor<br class="">
M?nchhausenstr. 21<br class="">
81247 M?nchen<br class="">
<br class="">
Besuchen Sie unsere Sammlung:<br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=IZN0n5RM-Km5GXICtvvAhdUH3exGNWvOIRMypAAUnQw&e=
<br class="">
<br class="">
---------<br class="">
<br class="">
Dirk Neumann<br class="">
<br class="">
Tel: +49-89-8107-111<br class="">
Fax: +49-89-8107-300<br class="">
email: Dirk.Neumann(a)zsm.mwn.de<br class="">
<br class="">
postal address:<br class="">
<br class="">
Bavarian Natural History Collections<br class="">
The Bavarian State Collection of Zoology<br class="">
Dirk Neumann, Section Ichthyology / DNA-Lab<br class="">
Muenchhausenstr. 21<br class="">
81247 Munich (Germany)<br class="">
<br class="">
Visit our section at:<br class="">
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=AwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=EQOvb7tfOYP7DFTGQe7aTcg8IbeMrUIFY3w22ylp7Jo&s=IZN0n5RM-Km5GXICtvvAhdUH3exGNWvOIRMypAAUnQw&e=
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<br class="">
Message: 4<br class="">
Date: Mon, 2 Feb 2015 10:08:52 -0500<br class="">
From: Christopher Kemp <cjkemp@gmail.com><br class="">
Subject: [Nhcoll-l] Please post this question<br class="">
To: nhcoll-l@mailman.yale.edu<br class="">
Message-ID:<br class="">
<span class="Apple-tab-span" style="white-space:pre"></span><CAFhqPyHvRXnyO_6VNOfaSyzbcC3-p1q1j33vm2YOCAd+wO9ZLA@mail.gmail.com><br class="">
Content-Type: text/plain; charset="utf-8"<br class="">
<br class="">
Hi All -- I've read a few species description papers lately in which the<br class="">
authors have described specimens' coloration with a color code, e.g.:<br class="">
Glaucous #80, Olive #29, etc. What's the source? Where can I get this code?<br class="">
Thanks -- ck<br class="">
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<br class="">
Message: 5<br class="">
Date: Mon, 2 Feb 2015 15:14:42 +0000<br class="">
From: Bob Chandler <bob.chandler@gcsu.edu><br class="">
Subject: Re: [Nhcoll-l] Please post this question<br class="">
To: Christopher Kemp <cjkemp@gmail.com>, "nhcoll-l@mailman.yale.edu"<br class="">
<span class="Apple-tab-span" style="white-space:pre"></span><nhcoll-l@mailman.yale.edu><br class="">
Message-ID:<br class="">
<span class="Apple-tab-span" style="white-space:pre"></span><BY2PR04MB87181144F3EFCB25B584001F83C0@BY2PR04MB871.namprd04.prod.outlook.com><br class="">
<span class="Apple-tab-span" style="white-space:pre"></span><br class="">
Content-Type: text/plain; charset="utf-8"<br class="">
<br class="">
Smithe, Frank B. ?Naturalist?s Color Guide.? AMNH 1975.<br class="">
<br class="">
Robert M. Chandler, Ph.D.<br class="">
Professor of Biology<br class="">
Coordinator of the GC Natural History Museum<br class="">
Department of Biological and Environmental Sciences<br class="">
Georgia College and State University<br class="">
CBX-081<br class="">
Milledgeville, GA 31061-0490<br class="">
<br class="">
<br class="">
<br class="">
<br class="">
From: nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu] On Behalf Of Christopher Kemp<br class="">
Sent: Monday, February 02, 2015 10:09 AM<br class="">
To: nhcoll-l@mailman.yale.edu<br class="">
Subject: [Nhcoll-l] Please post this question<br class="">
<br class="">
Hi All -- I've read a few species description papers lately in which the authors have described specimens' coloration with a color code, e.g.: Glaucous #80, Olive #29, etc. What's the source? Where can I get this code? Thanks -- ck<br class="">
<br class="">
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_______________________________________________<br class="">
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Nhcoll-l@mailman.yale.edu<br class="">
http://mailman.yale.edu/mailman/listinfo/nhcoll-l<br class="">
<br class="">
_______________________________________________ <br class="">
NHCOLL-L is brought to you by the Society for the Preservation of<br class="">
Natural History Collections (SPNHC), an international society whose<br class="">
mission is to improve the preservation, conservation and management of<br class="">
natural history collections to ensure their continuing value to<br class="">
society. See http://www.spnhc.org for membership information.<br class="">
<br class="">
<br class="">
End of Nhcoll-l Digest, Vol 34, Issue 1<br class="">
***************************************<br class="">
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</blockquote>
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