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<div class="moz-cite-prefix">Hi Andy,<br>
<br>
as we only have -80°C storage but not cryo (liquid nitrogen
storage), I cannot comment on the scissors performance per se, but
perhaps add a thought on "contamination"<br>
<br>
For us fish folks, but also for all people working with slimy,
mucus-rich critters, the mucus (or body fluids in case of fresh
[muscular] tissue samples) is probably No. 1 contaminant, which -
however - may already sit inside your storage fluid and inside
your tube because of original contamination issues in the field.
Using the same scissors - even if you clean them you can't dry
them completely, condensation on the cold scissors might add to
this problem - and immerge the same instrument into a couple of
fluid filled tubes seems to be the higher contamination risk.
Therefore, we prefer to remove the tissue, putting it on a neutral
surface (which is tossed away after usage) and to cut it
preferably with a scalpel blade.<br>
<br>
The second and much more immanent and critical contamination risk
occurs during extraction (and thus is completely independent of
tissue subsampling), especially when using extraction kits with
silica-column systems that do have strongly foam building washing
buffers. Normally, washing steps are done with larger volumes, and
- working at plate scale - with an electronic multichannel
pipette. I have seen folks that not only did not care about the
sipping and supping foam building up inside and outside the wells,
but wondering why they do have contaminations. The similar
applies, if pipettes are shared in and among working groups with
one person causing an undetected aerosol contamination inside the
pipette.<br>
<br>
If your tissue template used for extraction is sufficiently large,
there should be no contamination issue (unless your the DNA of
your template is completely degraded and/or the DNA yield fairly
small), as the DNA of the template will always outnumber potential
strains. But fluid transfer and contamination of DNA molecules
dissolved to it may be the much higher risk.<br>
<br>
Hope this helps even though it does not directly answer your
original question.<br>
<br>
Dirk<br>
<br>
<br>
Am 09.06.2015 um 18:27 schrieb Bentley, Andrew Charles:<br>
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<p class="MsoNormal">Hi all<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I am interested in knowing what others are
using for subsampling tissues for tissue gift purposes in
their cryo collections. I know some people use scalpel blades
but I prefer fine dissection scissors as they not only provide
a finer manipulation but also (in most cases) negate having to
remove the tissue from the original tube an thus avoid
additional contamination possibilities.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have been having issues however with the
regular dissection scissors we have been using as frozen
tissue (especially at cryo temps) causes a large amount of
wear and tear (both dulling and bending) of these scissors.
So, we are considering purchasing ceramic coated tungsten
carbide scissors such as these: <a moz-do-not-send="true" href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.medicalresources.com_shopping_proddisplay.asp-3Fcatalogid-3D18330&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=YH6kgdVNvngaC4BvStezXfuEIUtApvrk-vEQfUz0Y1M&s=hvDxlayAy9zEc7ZCtwus8kvPYWZbParjjK-l_WuSmdw&e=">http://www.medicalresources.com/shopping/proddisplay.asp?catalogid=18330</a>
– which are purported to be six times more durable than
regular stainless steel varieties. They are however VERY
expensive and so, before diving in I thought I would ask
around to see what others are using.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Any assistance would be greatly
appreciated.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Andy<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"
lang="EN-AU"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"
lang="EN-AU">Dyche Hall<br>
1345 Jayhawk Boulevard<br>
Lawrence, KS, 66045-7561<br>
USA<br>
<br>
Tel: (785) 864-3863<br>
Fax: (785) 864-5335 <br>
Email: <a moz-do-not-send="true" href="mailto:abentley@ku.edu"><span style="color:blue">abentley@ku.edu</span></a> <o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"
lang="EN-AU"><a moz-do-not-send="true" href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=YH6kgdVNvngaC4BvStezXfuEIUtApvrk-vEQfUz0Y1M&s=Bqu7wN4vr5Db4okSolzNRd9YpasDvLtpDPVi9VucDrw&e="><span
style="color:blue">http://ichthyology.biodiversity.ku.edu</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"
lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"
lang="EN-AU">SPNHC President<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"
lang="EN-AU"><a moz-do-not-send="true" href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=YH6kgdVNvngaC4BvStezXfuEIUtApvrk-vEQfUz0Y1M&s=MzrN-vtPKotJ2jD6e4eSRzNLVIlUqZmDJrwSj2kp1pw&e="><span
style="color:blue">http://www.spnhc.org</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span
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<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"
lang="EN-AU"> :
: <br>
A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V</span><span
style="color:#1F497D" lang="EN-AU"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<pre class="moz-signature" cols="72">--
Dirk Neumann
Tel: 089 / 8107-111
Fax: 089 / 8107-300
email: Dirk.Neumann(a)zsm.mwn.de
Postanschrift:
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---------
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Fax: +49-89-8107-300
email: Dirk.Neumann(a)zsm.mwn.de
postal address:
Bavarian Natural History Collections
The Bavarian State Collection of Zoology
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