<html><head><meta http-equiv="Content-Type" content="text/html charset=iso-8859-1"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">A pleasure to help.<div><br><div><div>I would like to get some of the OT aerosol to try out for myself but being in the UK means that you have to purchase a metric tonne or just get a data sheet, not very helpful! Any ideas where I might be able to get some - a normal amount?</div><div><br><div apple-content-edited="true">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">With all good wishes, Simon.</div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br></div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Simon Moore MIScT, RSci, FLS, ACR<br>Conservator of Natural Sciences and Cutlery Historian,<br><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FwcYvotkr23Aq08PA-QTUxUnAP_tpbIRagA_3t40dZI&s=ZFwmEiGl2kq_DxbpNmeX6t6M17sg1oEMJPOjWoMZ4ds&e=">www.natural-history-conservation.com</a> </div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br><br><br></div></div></div>
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<br><div><div>On 25 Jun 2015, at 18:06, Yemisi Dare <<a href="mailto:YDare@mus-nature.ca">YDare@mus-nature.ca</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div lang="EN-CA" link="blue" vlink="purple" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div class="WordSection1" style="page: WordSection1;"><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);">Thank you very much Eric and Simon, for these pointers!<o:p></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);"> </span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);">Yemisi.<o:p></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);"> </span></div><div><div style="border-style: solid none none; border-top-color: rgb(181, 196, 223); border-top-width: 1pt; padding: 3pt 0cm 0cm;"><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><b><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif;">From:</span></b><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif;"><span class="Apple-converted-space"> </span>Simon Moore [<a href="mailto:couteaufin@btinternet.com">mailto:couteaufin@btinternet.com</a>]<span class="Apple-converted-space"> </span><br><b>Sent:</b><span class="Apple-converted-space"> </span>June-25-15 12:43 PM<br><b>To:</b><span class="Apple-converted-space"> </span>Lazo-Wasem, Eric<br><b>Cc:</b><span class="Apple-converted-space"> </span>Yemisi Dare; Smith, Jamie; <a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br><b>Subject:</b><span class="Apple-converted-space"> </span>Re: [Nhcoll-l] Rehydrating crustaceans<o:p></o:p></span></div></div></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><o:p> </o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;">The OT spray is Na di-octyl sulpho-succinate - now in a convenient spray, should be good and many thanks for the update.<o:p></o:p></div><div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><o:p> </o:p></div><div><div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="">With all good wishes, Simon.<o:p></o:p></span></div></div><div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style=""> </span></div></div><div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="">Simon Moore MIScT, RSci, FLS, ACR<br>Conservator of Natural Sciences and Cutlery Historian,<br><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FwcYvotkr23Aq08PA-QTUxUnAP_tpbIRagA_3t40dZI&s=TIxLXmwpsUi5biknga4R0jCwxfHaky6pu7kxbeU4zZ4&e=" style="color: purple; text-decoration: underline;">www.natural-history-conservation.com</a> <o:p></o:p></span></div></div><div><p class="MsoNormal" style="margin: 0cm 0cm 12pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style=""><br><br><o:p></o:p></span></p></div></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><o:p> </o:p></div><div><div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;">On 25 Jun 2015, at 16:53, Lazo-Wasem, Eric <<a href="mailto:eric.lazo-wasem@yale.edu" style="color: purple; text-decoration: underline;">eric.lazo-wasem@yale.edu</a>> wrote:<o:p></o:p></div></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><br><br><o:p></o:p></div><div><div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="font-size: 9pt;">Dear Yemesi,<br><br>I have generally placed the dried out material in a small container (capped vial, watch glass, etc.) along with a small volume of the wetting agent and let it sit overnight. I continue monitoring daily until the crustacean feels flexible and there are no more air bubbles visible inside the exoskeleton. I have actually forgotten samples and left them sit for more than a week, but in practice I think it usually takes 3-4 days for small things (amphipods, copepods) and a good week or so for something larger (such as the leeches I mentioned). The trick is to monitor regularly and when it appears "done" you can go back straight into ethanol (70-75% is what we use).<br><br>Eric LW<br><br><br><br><br>-----Original Message-----<br>From: Yemisi Dare [<a href="mailto:YDare@mus-nature.ca" style="color: purple; text-decoration: underline;">mailto:YDare@mus-nature.ca</a>]<span class="apple-converted-space"> </span><br>Sent: Thursday, June 25, 2015 11:43 AM<br>To: Lazo-Wasem, Eric; Smith, Jamie<br>Subject: RE: Rehydrating crustaceans<br><br>Dear Eric, Jamie,<br><br>We have precisely the same issue here and I have been looking for a resource somewhere on how to rehydrate some of our crustacean donations that are severely dessicated.<br>Eric do you have a written protocol that I can use?<br><br>Thank you very much,<br><br>Yemisi Dare.<br><br>Yemisi Dare, Ph.D.<br>Assistant Collections Manager, Invertebrates / Gestionnaire adjointe des collections invertébrés Canadian Museum of Nature / Musée canadien de la Nature PO Box 3443 Station D / CP 3443 Succ <<D>> Ottawa, ON Canada K1P 6P4<br><br>Tel: 613-566 4736/ F: 613-364-4027<br>E-Mail:<span class="apple-converted-space"> </span><a href="mailto:ydare@mus-nature.ca" style="color: purple; text-decoration: underline;">ydare@mus-nature.ca</a><br><br><br>-----Original Message-----<br>From:<span class="apple-converted-space"> </span><a href="mailto:nhcoll-l-bounces@mailman.yale.edu" style="color: purple; text-decoration: underline;">nhcoll-l-bounces@mailman.yale.edu</a><span class="apple-converted-space"> </span>[<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" style="color: purple; text-decoration: underline;">mailto:nhcoll-l-bounces@mailman.yale.edu</a>] On Behalf Of Lazo-Wasem, Eric<br>Sent: June-25-15 11:30 AM<br>To: Smith, Jamie<br>Cc:<span class="apple-converted-space"> </span><a href="mailto:nhcoll-l@mailman.yale.edu" style="color: purple; text-decoration: underline;">nhcoll-l@mailman.yale.edu</a><br>Subject: Re: [Nhcoll-l] Rehydrating crustaceans<br><br>Dear Jamie,<br><br>I have been using "Aerosol OT," a wetting agent suggested to me by Bill Moser at the NMNH. We have successfully been rehydrating tough as leather dried out leeches (140 years old) and amphipod crustaceans (types dried out from 1871). The latter rehydrate so well that I can dissect the specimens and make permanent stained mounts in balsam. Even plankton sample more than 50 years old have been rehydrated, and we can successfully mount copepods and daphniids after a weeks soak in A-OT.<br><br>An alternative is Tri-basic calcium phosphate, or the old house painters washing solution, TSP (tri-sodium phosphate). Neither of these work nearly as well as Aerosol OT. <br><br>Eric Lazo-Wasem<br>Yale Peabody Museum<br><br><br><br>-----Original Message-----<br>From:<span class="Apple-converted-space"> </span><a href="mailto:nhcoll-l-bounces@mailman.yale.edu" style="color: purple; text-decoration: underline;">nhcoll-l-bounces@mailman.yale.edu</a><span class="Apple-converted-space"> </span>[<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" style="color: purple; text-decoration: underline;">mailto:nhcoll-l-bounces@mailman.yale.edu</a>] On Behalf Of Smith, Jamie<br>Sent: Thursday, June 25, 2015 11:04 AM<br>To:<span class="Apple-converted-space"> </span><a href="mailto:nhcoll-l@mailman.yale.edu" style="color: purple; text-decoration: underline;">nhcoll-l@mailman.yale.edu</a><br>Subject: [Nhcoll-l] Rehydrating crustaceans<br><br>Has anyone had any luck rehydrating crustaceans that have completely dried out? If so, which method did you use?<br><br><br><br><br><br>Jamie M. 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