<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi Mare, <div><br></div><div>A although I haven’t yet tried the Singer technique for rehydration but (in theory) it should work well. You might have to try it out first as bracteal cells can be prone to uneven rehydration and might lead to detachments just after the process</div><div><br></div><div>The usual preservative for plant materiaL is Copenhagen mixture:
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<!--StartFragment--><p class="MsoNormal"><font face="Bookman Old Style"><span style="font-size: 9pt; line-height: 11.4pt;">Copenhagen mixture:
IMS/ethanol (76), distilled water (18), glycerol (5). Make sure that this is </span><span style="line-height: 15px;">compounded</span><span style="font-size: 9pt; line-height: 11.4pt;"> at least 24 hours before use as the </span><span style="line-height: 15px;">mixture</span><span style="font-size: 9pt; line-height: 11.4pt;"> evolves many tiny air bubbles during the mixing!</span></font></p><p class="MsoNormal"><font face="Bookman Old Style"><span style="font-size: 9pt; line-height: 11.4pt;">FAA is used more for plant histology these days and to be used more frequently, as a fixative, back in the 60</span><span style="line-height: 15px;">’</span><span style="font-size: 9pt; line-height: 11.4pt;">s and 70</span><span style="line-height: 15px;">’</span><span style="font-size: 9pt; line-height: 11.4pt;">s but became superseded by Kew mixture later on as the latter is more user friendly.</span></font></p>
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<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">With all good wishes, Simon.</div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br></div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Simon Moore MIScT, RSci, FLS, ACR<br>Conservator of Natural Sciences and Cutlery Historian,<br><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=AwMF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=ecTUqHGUyHLmhdMsmsKO1DXGdpWYI4xkabOao16Lw4Y&s=_K5kNKLW92gpkRrnA4Tp0a_GB0_kNbm2l3C57Ab-47A&e=">www.natural-history-conservation.com</a> </div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br><br><br></div></div></div>
</div>
<br><div><div>On 31 Jul 2015, at 19:00, Mare Nazaire <<a href="mailto:mnazaire@rsabg.org">mnazaire@rsabg.org</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div dir="ltr">I too have a very similar issue, but with plant specimens. We have a number of fluid preserved specimens of plants that are quite old and rather brittle, and have been desiccated for quite some time. Can Singer's technique also work for desiccated plant specimens?<div><br></div><div>Similarly, for old collections of fluid preserved plant specimens that need to be "topped off" - what concentration of ethyl alcohol is recommended? I am guessing that these have been preserved in FAA, but some specimens are decades old, and I am not certain as to what was used back then to preserve specimens.</div><div><br></div><div>Thanks in advance,</div><div>~Mare</div></div><div class="gmail_extra"><br><div class="gmail_quote">On Fri, Jul 31, 2015 at 8:50 AM, David Dyer <span dir="ltr"><<a href="mailto:DDyer@ohiohistory.org" target="_blank">DDyer@ohiohistory.org</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div lang="EN-US" link="blue" vlink="purple">
<div><p class="MsoNormal">We’re working with a county historical society that has an old vertebrate specimen, and the fluid that it was preserved in has long-since evaporated. The specimen is from 1881 and has been on exhibit for about 30 years. They think it was
in formaldehyde (formalin). The curator agrees that it should now be taken off exhibit, and have asked us to advise on how to preserve. Is it OK to rehydrate in ethyl alcohol (70%) or are there other steps that are recommended?
<u></u><u></u></p><p class="MsoNormal">Thanks in advance for any advice!<u></u><u></u></p><p class="MsoNormal"><u></u> <u></u></p><p class="MsoNormal">Dave<u></u><u></u></p><p class="MsoNormal"><u></u> <u></u></p><p class="MsoNormal">==========================================================<u></u><u></u></p><p class="MsoNormal"><b>David L. Dyer / Curator of Natural History</b>
<u></u><u></u></p><p class="MsoNormal">Ohio History Connection / 800 East 17<sup>th</sup> Ave. Columbus, Ohio 43211
<i><u></u><u></u></i></p><p class="MsoNormal">p. <a href="tel:614-298-2055" value="+16142982055" target="_blank">614-298-2055</a> / f. <a href="tel:614-298-2089" value="+16142982089" target="_blank">614-298-2089</a> / <u><a href="mailto:ddyer@ohiohistory.org" target="_blank">ddyer@ohiohistory.org</a></u><i><u></u><u></u></i></p><p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.facebook.com_ohiohistoryconnection&d=AwMFAg&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Q-ts5icCfYCk1klrFNSSmLbbcJg3oPm4k0xcF4wAMnc&s=QJ2aqxuSjxEbqw4M3wT3ONUBJRJgbOQgvpDS4tTL4GU&e=" target="_blank"><span style="color:blue;text-decoration:none"><image001.gif></span></a><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__twitter.com_OhioHistory&d=AwMFAg&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Q-ts5icCfYCk1klrFNSSmLbbcJg3oPm4k0xcF4wAMnc&s=aQVfTCLqwJ8L_l7As-e7i1zpZbpTqcwlwGZjm31gp4Y&e=" target="_blank"><span style="color:blue;text-decoration:none"><image002.gif></span></a><u></u><u></u></p><p class="MsoNormal">Planning a road trip? Check out our more than 50 historic sites at the new mobile-friendly
<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ohiohistory.org_&d=AwMFAg&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Q-ts5icCfYCk1klrFNSSmLbbcJg3oPm4k0xcF4wAMnc&s=YXO7NDcC-JJyXVykC0pTB3A3ElZQw4cgIk7gv_I8doM&e=" target="_blank"><span style="color:blue">ohiohistory.org</span></a><b>.<u></u><u></u></b></p><p class="MsoNormal"><u></u> <u></u></p><p class="MsoNormal"><i>Visit the Natural History Blog at</i>: <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__apps.ohiohistory.org_naturalhistory_&d=AwMFAg&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Q-ts5icCfYCk1klrFNSSmLbbcJg3oPm4k0xcF4wAMnc&s=vqjS4EW6PtoHkAehlrK2x2aEyChYAChe7DwYpcAeLEU&e=" target="_blank">
<span style="color:blue">http://apps.ohiohistory.org/naturalhistory/</span></a><u></u><u></u></p><p class="MsoNormal"><u></u> <u></u></p><p class="MsoNormal"><u></u> <u></u></p><p class="MsoNormal"><u></u> <u></u></p>
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<br></blockquote></div><br><br clear="all"><div><br></div>-- <br><div class="gmail_signature"><div dir="ltr"><span style="font-family: Tahoma; border-collapse: separate;"><font face="Helvetica" size="2">Mare Nazaire, Ph.D.</font><font face="Helvetica" size="2"><br>Collections Manager, Herbarium [RSA-POM]</font><font face="Helvetica" size="2"><br></font></span><span style="font-family: Tahoma; border-collapse: separate;"><font face="Helvetica" size="2">Rancho Santa Ana Botanic Garden</font><font face="Helvetica" size="2"><br></font></span><div><span style="border-collapse: separate;">1500 North College Avenue<br>Claremont, California 91711</span></div><div><span style="border-collapse: separate;">909.625.8767 ext. 268</span></div><div></div></div></div>
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_______________________________________________<br>Nhcoll-l mailing list<br><a href="mailto:Nhcoll-l@mailman.yale.edu">Nhcoll-l@mailman.yale.edu</a><br>http://mailman.yale.edu/mailman/listinfo/nhcoll-l<br><br>_______________________________________________ <br>NHCOLL-L is brought to you by the Society for the Preservation of<br>Natural History Collections (SPNHC), an international society whose<br>mission is to improve the preservation, conservation and management of<br>natural history collections to ensure their continuing value to<br>society. See http://www.spnhc.org for membership information.<br>Advertising on NH-COLL-L is inappropriate.<br></blockquote></div><br></div></body></html>