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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Sarah<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Although I don’t have any data or publications to back it up I think you are correct. There is very little difference between 95% and 100% ethanol and the minimal
water content in 95% would have little to no effect on DNA preservation. That being said, both of these will degrade DNA over time and as such we only use this as a transportation fluid when shipping tissues or for field collection where liquid nitrogen is
out of the question. All tissues that come into our collection have all fluids drained off before they are placed in our liquid nitrogen dewars to ensure that no further degradation of DNA occurs due to them still being in the fluid. It also ensures that
the piece of tissue is accessible and you do not have to hunt through frozen to half frozen fluid to find it in the tube.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">I have attached publications that talk about extraction of DNA from 95% ethanol preserved specimens.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Hope that helps<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Andy<o:p></o:p></span></p>
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Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">Dyche Hall<br>
1345 Jayhawk Boulevard<br>
Lawrence, KS, 66045-7561<br>
USA<br>
<br>
Tel: (785) 864-3863<br>
Fax: (785) 864-5335 <br>
Email: <a href="mailto:abentley@ku.edu"><span style="color:blue">abentley@ku.edu</span></a> <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=tkstNReNxyApvBBLzDva75h-cKu9Ktt4AsedoMpGhlo&s=pTILctX8oWmMqsR6r_D98bEjUtZdcpU8JK3x5RznX-E&e="><span style="color:blue">http://ichthyology.biodiversity.ku.edu</span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">SPNHC President<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=tkstNReNxyApvBBLzDva75h-cKu9Ktt4AsedoMpGhlo&s=KsjryG6dflOTPZ2dRcFmaAgJS5IWD3ahC8X5RzOahEc&e="><span style="color:blue">http://www.spnhc.org</span></a><o:p></o:p></span></p>
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A : A : A :<br>
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V V V</span><span lang="EN-AU" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Sarah K. Huber<br>
<b>Sent:</b> Wednesday, January 27, 2016 11:34 AM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Cc:</b> Eric Hilton <ehilton@vims.edu><br>
<b>Subject:</b> [Nhcoll-l] Ethanol concentration for tissue samples<o:p></o:p></span></p>
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<p><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">I recently had a graduate student request 100% absolute ethanol to use for preserving tissue samples (fish muscle and/or fins). We routinely use 95% ethanol (and all tissue samples
are then stored in a -80 freezer); however, the student is adamant that 100% is the only preservative that is acceptable for tissue preservation. Does anyone have an opinion on 95% versus 100% ethanol for tissue preservation? There is a very steep price difference
between the two fluids, and my understanding is that 95% ethanol preserves tissues just as well as 100%.<o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"> <o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">Thanks,<o:p></o:p></span></p>
<p><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">Sarah<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><br>
</span><span style="font-family:"Tahoma",sans-serif;color:black">Sarah K. Huber, Ph.D.</span><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><br>
Curatorial Associate, VIMS Nunnally Ichthyology Collection<br>
Office 804.684.7104 | Collection 804.684.7285<br>
<a href="mailto:skhuber@vims.edu">skhuber@vims.edu</a> | <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.vims.edu&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=tkstNReNxyApvBBLzDva75h-cKu9Ktt4AsedoMpGhlo&s=dlz5SX_rzd15h39vpXxoMCb-SL62LiWyc4o8UAwpwxE&e=">
www.vims.edu</a><br>
PO Box 1346 | 1375 Greate Rd., Gloucester Pt., VA 23062<o:p></o:p></span></p>
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