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<div><span style="background-color: rgba(255, 255, 255, 0);">When you add tissue to ethanol it is immediately diluted by the water in the tissue so achieving 100% is not possible. In my opinion 95 % should be fine. One method I have used us to pour off the
diluted ethanol from the sample then refill with fresh to bring up the concentration.</span></div>
<div><span style="background-color: rgba(255, 255, 255, 0);"><br>
Paul Sweet | Department of Ornithology | American Museum of Natural History | Central Park West @ <a dir="ltr" href="x-apple-data-detectors://1" x-apple-data-detectors="true" x-apple-data-detectors-type="address" x-apple-data-detectors-result="1">79th St |
NY 10023</a> | Tel <a dir="ltr" href="tel:212%20769%205780" x-apple-data-detectors="true" x-apple-data-detectors-type="telephone" x-apple-data-detectors-result="2">212 769 5780</a> | Mob <a dir="ltr" href="tel:718%20757%205941" x-apple-data-detectors="true" x-apple-data-detectors-type="telephone" x-apple-data-detectors-result="3">718
757 5941</a></span></div>
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On Jan 27, 2016, at 12:34 PM, Sarah K. Huber <<a href="mailto:skhuber@vims.edu">skhuber@vims.edu</a>> wrote:<br>
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<p><font color="#000000"><span style="background-color: rgba(255, 255, 255, 0);">I recently had a graduate student request 100% absolute ethanol to use for preserving tissue samples (fish muscle and/or fins). We routinely use 95% ethanol (and all tissue samples
are then stored in a -80 freezer); however, the student is adamant that 100% is the only preservative that is acceptable for tissue preservation. Does anyone have an opinion on 95% versus 100% ethanol for tissue preservation? There is a very steep price difference
between the two fluids, and my understanding is that 95% ethanol preserves tissues just as well as 100%.</span></font></p>
<p><font color="#000000"><span style="background-color: rgba(255, 255, 255, 0);"> </span></font></p>
<p><font color="#000000"><span style="background-color: rgba(255, 255, 255, 0);">Thanks,</span></font></p>
<p><font color="#000000"><span style="background-color: rgba(255, 255, 255, 0);">Sarah</span></font></p>
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<div><font color="#000000"><span style="background-color: rgba(255, 255, 255, 0);"><br>
Sarah K. Huber, Ph.D.<br>
Curatorial Associate, VIMS Nunnally Ichthyology Collection<br>
Office <a dir="ltr" href="tel:804.684.7104" x-apple-data-detectors="true" x-apple-data-detectors-type="telephone" x-apple-data-detectors-result="7">804.684.7104</a> | Collection <a dir="ltr" href="tel:804.684.7285" x-apple-data-detectors="true" x-apple-data-detectors-type="telephone" x-apple-data-detectors-result="8/0">804.684.</a><a dir="ltr" href="tel:804.684.7285" x-apple-data-detectors="true" x-apple-data-detectors-type="telephone" x-apple-data-detectors-result="8/0">7285</a><br>
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