<html><head><meta http-equiv="Content-Type" content="text/html charset=iso-8859-1"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi Sarah,<div><br></div><div><br></div><div>Presumably this relates to preservation of DNA? If so, then it was agreed, a few years ago, that using 96% ethanol and stored at 5 deg. C was the best. 99% and absolute were found to be less effective.</div><div>I am sure there will be other comments relating to this.</div><div><br><div apple-content-edited="true">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">With all good wishes, Simon.</div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br></div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Simon Moore MIScT, RSci, FLS, ACR<br>Conservator of Natural Sciences and Cutlery Historian,<br><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=MdgXxloTDHBxPRNNRBDOtAs10obcg32_edRdQjHAD7U&s=rl1pZGY6sHOn5Tz9XTlu0JlqWEQ5y0JA4HMvMWtpVE0&e=">www.natural-history-conservation.com</a> </div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br><br><br></div></div></div>
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<br><div><div>On 27 Jan 2016, at 17:34, Sarah K. Huber <<a href="mailto:skhuber@vims.edu">skhuber@vims.edu</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div fpstyle="1" ocsi="0" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="direction: ltr; font-family: Tahoma; font-size: 10pt;"><div style="margin-bottom: 0px; margin-top: 0px;">I recently had a graduate student request 100% absolute ethanol to use for preserving tissue samples (fish muscle and/or fins). We routinely use 95% ethanol (and all tissue samples are then stored in a -80 freezer); however, the student is adamant that 100% is the only preservative that is acceptable for tissue preservation. Does anyone have an opinion on 95% versus 100% ethanol for tissue preservation? There is a very steep price difference between the two fluids, and my understanding is that 95% ethanol preserves tissues just as well as 100%.</div><p style="margin-bottom: 0px; margin-top: 0px;"> </p><div style="margin-bottom: 0px; margin-top: 0px;">Thanks,</div><div style="margin-bottom: 0px; margin-top: 0px;">Sarah</div><div style="font-size: 13px; font-family: Tahoma;"><div style="font-size: 13px; font-family: Tahoma;"><div style="font-size: 13px; font-family: Tahoma;"><font size="3"></font><div style="font-size: 13px; font-family: Tahoma;"><font size="3"><br></font><font size="2"><span style="font-size: 10pt;"><font size="3">Sarah K. Huber, Ph.D.</font><br></span></font><font size="2"><span style="font-size: 10pt;">Curatorial Associate, VIMS Nunnally Ichthyology Collection<br></span></font><font size="2"><span style="font-size: 10pt;">Office 804.684.7104<span class="Apple-converted-space"> </span><span style="font-size: 10pt;">| Collection 804.684.</span>7285<br><a href="mailto:skhuber@vims.edu">skhuber@vims.edu</a><span class="Apple-converted-space"> </span><font size="2"><span style="font-size: 10pt;">|<span class="Apple-converted-space"> </span><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.vims.edu_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=MdgXxloTDHBxPRNNRBDOtAs10obcg32_edRdQjHAD7U&s=nQ4VlG-6aQdQrNtdGud1rIHqnzPfxZ3SuLCT8C8qxYE&e=">www.vims.edu</a></span></font></span></font><br><font size="2"><span style="font-size: 10pt;">PO Box 1346 | 1375 Greate Rd., Gloucester Pt., VA 23062</span></font></div></div></div></div></div>_______________________________________________<br>Nhcoll-l mailing list<br><a href="mailto:Nhcoll-l@mailman.yale.edu">Nhcoll-l@mailman.yale.edu</a><br><a href="http://mailman.yale.edu/mailman/listinfo/nhcoll-l">http://mailman.yale.edu/mailman/listinfo/nhcoll-l</a><br><br>_______________________________________________<span class="Apple-converted-space"> </span><br>NHCOLL-L is brought to you by the Society for the Preservation of<br>Natural History Collections (SPNHC), an international society whose<br>mission is to improve the preservation, conservation and management of<br>natural history collections to ensure their continuing value to<br>society. See<span class="Apple-converted-space"> </span><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=MdgXxloTDHBxPRNNRBDOtAs10obcg32_edRdQjHAD7U&s=WZC69hsK5Mltqh9mAvFdMksFPPqGdXNlyttmcDPyI1k&e=">http://www.spnhc.org</a><span class="Apple-converted-space"> </span>for membership information.<br>Advertising on NH-COLL-L is inappropriate.</div></blockquote></div><br></div></body></html>