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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">As Simon points out, it depends what you want the specimen for. On the one hand, in our experience clean DNA (single genes or the whole thing) can be got from
specimens in concentrations as low as 70% (though there is some variation between organisms); on the other, 95% will shrink and harden a specimen, making it difficult to do anatomical work without rehydration that itself damages cells. Absolute alcohol (99.9%)
has been denatured using chemicals, as distillation only gets purity up to 95%. Any trace denaturant might have unpredictable long-term effects on the specimen.
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">PC<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:10.0pt;font-family:"Calibri","sans-serif";color:black">Paul Callomon</span></b><span style="font-size:10.0pt;font-family:"Calibri","sans-serif";color:black"><br>
<i>Collection Manager, Malacology, Invertebrate Paleontology and General Invertebrates</i></span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:10.0pt;font-family:"Calibri","sans-serif";color:black">Academy of Natural Sciences of Drexel University, Philadelphia</span></b><b><span style="font-size:10.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></b></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Calibri","sans-serif";color:black">1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA</span><span style="font-size:10.0pt;font-family:"Calibri","sans-serif";color:black"><br>
<i><a href="mailto:callomon@ansp.org" target="_blank">callomon@ansp.org</a> Tel 215-405-5096 - Fax 215-299-1170</i></span><span style="font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Simon Moore<br>
<b>Sent:</b> Wednesday, January 27, 2016 12:47 PM<br>
<b>To:</b> Sarah K. Huber<br>
<b>Cc:</b> nhcoll-l@mailman.yale.edu; Eric Hilton<br>
<b>Subject:</b> Re: [Nhcoll-l] Ethanol concentration for tissue samples<o:p></o:p></span></p>
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<p class="MsoNormal">Hi Sarah,<o:p></o:p></p>
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<p class="MsoNormal">Presumably this relates to preservation of DNA? If so, then it was agreed, a few years ago, that using 96% ethanol and stored at 5 deg. C was the best. 99% and absolute were found to be less effective.<o:p></o:p></p>
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<p class="MsoNormal">I am sure there will be other comments relating to this.<o:p></o:p></p>
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<p class="MsoNormal"><span style="color:black">With all good wishes, Simon.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black">Simon Moore MIScT, RSci, FLS, ACR<br>
Conservator of Natural Sciences and Cutlery Historian,<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=MdgXxloTDHBxPRNNRBDOtAs10obcg32_edRdQjHAD7U&s=rl1pZGY6sHOn5Tz9XTlu0JlqWEQ5y0JA4HMvMWtpVE0&e=">www.natural-history-conservation.com</a> <o:p></o:p></span></p>
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<p class="MsoNormal">On 27 Jan 2016, at 17:34, Sarah K. Huber <<a href="mailto:skhuber@vims.edu">skhuber@vims.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">I recently had a graduate student request 100% absolute ethanol to use for preserving tissue samples (fish muscle and/or fins). We routinely use 95% ethanol (and all tissue
samples are then stored in a -80 freezer); however, the student is adamant that 100% is the only preservative that is acceptable for tissue preservation. Does anyone have an opinion on 95% versus 100% ethanol for tissue preservation? There is a very steep
price difference between the two fluids, and my understanding is that 95% ethanol preserves tissues just as well as 100%.<o:p></o:p></span></p>
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<p style="margin:0in;margin-bottom:.0001pt"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">Thanks,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">Sarah<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Tahoma","sans-serif""><br>
Sarah K. Huber, Ph.D.</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""><br>
Curatorial Associate, VIMS Nunnally Ichthyology Collection<br>
Office 804.684.7104<span class="apple-converted-space"> </span>| Collection 804.684.7285<br>
<a href="mailto:skhuber@vims.edu">skhuber@vims.edu</a><span class="apple-converted-space"> </span>|<span class="apple-converted-space"> </span><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.vims.edu_&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=MdgXxloTDHBxPRNNRBDOtAs10obcg32_edRdQjHAD7U&s=nQ4VlG-6aQdQrNtdGud1rIHqnzPfxZ3SuLCT8C8qxYE&e=">www.vims.edu</a><br>
PO Box 1346 | 1375 Greate Rd., Gloucester Pt., VA 23062<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif"">_______________________________________________<br>
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