<div dir="ltr">Hello everyone,<div><br></div><div>In the near the future, the UWYMV will be working to house samples of Wyoming Toads (<i>Anaxyrus baxteri</i>) from captive breeding programs.</div><div>There is a large back log of toads from previous years in a chest freezer, and several things we need to consider is how viable is the material still available in the amphibians that have been in long storage, and also the best way to preserve them.</div><div><br></div><div>Does anyone have experience with extracting and sequencing samples from whole specimens that have been stored for 10+ years in freezers that are kept at ~10C? Did the storage in the freezers effect the specimens or samples in any way?</div><div><br></div><div>Since the genetic material from these specimens are potentially very valuable, we're thinking of taking a tissue sample, and then fixing the specimens in ETOH so they can be sampled later. I have fixed bird and mammal carcasses in 95% ETOH and then transferred to 70% ETOH for storage, but all of the herps I have prepared have been fixed in formaldehyde. </div><div><br></div><div>Does anyone have a experience or a protocol for fixing amphibians in ETOH for long term storage?</div><div><br></div><div>As always, a big thank you to everyone for any advice and help.</div><div><br></div><div>cheers,</div><div>Beth Wommack<br clear="all"><div><br></div>-- <br><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr">Elizabeth Wommack, PhD<br>Curator and Collections Manager of Vertebrates<br>University of Wyoming Museum of Vertebrates<div>Berry Biodiversity Conservation Center<div>University of Wyoming, Laramie<br>Laramie, WY 82071<br><a href="mailto:ewommack@berkeley.edu" target="_blank">ewommack@</a><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__uwyo.edu&d=CwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=1nIibaBC_vkSHt2cf61FJC1_ZvLrwN8aQN9XwNdEpIg&s=vWymA66T1SHKJGnMd0hicYeSTMavCKL9C8VXl2Qjs8I&e=" target="_blank">uwyo.edu</a></div></div><div><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.uwymv.org&d=CwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=1nIibaBC_vkSHt2cf61FJC1_ZvLrwN8aQN9XwNdEpIg&s=Q8N0M-dMhIgpxZ0BVtPSszC42c3uYVz7A85p0ajoD1A&e=" target="_blank">www.uwymv.org</a></div></div></div></div></div>
</div></div>