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<div class="moz-cite-prefix">Dear Nicolas,<br>
<br>
<br>
it might be worth to compare the attached paper; my colleague did
some tests which are summarized therein. Normal pH-paper tests
will not work, you need to dilute the alcohol to get reliable
measurements (see attached paper).<br>
<br>
If your tap water is not chlorinated and pure but calciferous, you
might dilute your 96% EthOH with tap water to 80%; the dilution
needs to rest for at least one day, as calcium carbonate starts to
precipitate; the residual carbonate in solution gives you some
sort of buffer capacity.<br>
<br>
Hope this helps<br>
Dirk<br>
<br>
<br>
Am 18.10.2016 um 16:00 schrieb Margraf Nicolas:<br>
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<p class="MsoPlainText"><span style="font-family:"Courier
New"" lang="EN-US">Dear all,<o:p></o:p></span></p>
<p class="MsoPlainText"><span style="font-family:"Courier
New"" lang="EN-US">The MHNC is a small natural history
museum located in the hearth of the Jura Mountains in La
Chaux-de-Fonds, Switzerland. The MHNC team is currently
re-conditioning the alcohol collection, changing containers
and alcohol using an 80% Ethanol solution. The team was told
to check the acidity of the alcoholic solution as acidity is
not good for the specimens but had trouble doing so.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"" lang="EN-US">Is a 9.6 mg/l acidity expressed as
acetic acid too acidic? (12 mg/l in 96% Et-OH diluted to
4/5)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"" lang="EN-US">Additional questions:<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"" lang="EN-US">The alcohol dealer was asked about
the acidity of the 96% Ethanol he provides us with. The
value he gave us is 12 mg/l of acidity expressed as acetic
acid. We tried to calculate the pH based on that value and
obtain a value of 4.23. Does that sound right?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"" lang="EN-US">We tried measuring the pH of the
solution with both pH-paper and a pH-meter and found
conflicting values. Is the pH-paper reading valid for an
alcoholic solution? Or is the pH-meter value valid for an
alcoholic solution?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"" lang="EN-US">A pH of 4.23 seems too acidic to
preserve specimens so we tried to calculate the amount of
Na2CO3 necessary to neutralize the 12 mg/l of CH3COOH and
obtain 10.6 mg/l. Is it correct (and sufficient) to add this
amount of Na2CO3 to every liter of our 96% Et-OH solution?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"" lang="EN-US">Any advice or help would be greatly
appreciated.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">Thanks,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">Sincerely,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">N<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">*****************************************<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">Nicolas Margraf <o:p>
</o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">Collaborateur scientifique<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">Musée d'histoire naturelle de La Chaux-de-Fonds<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">Av. Léopold-Robert 63 - CH 2300 LA CHAUX-DE-FONDS<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New"">T +41 (0)32 967 60 71 -
<a moz-do-not-send="true" href="mailto:mhnc@ne.ch"><span
style="color:blue">mhnc@ne.ch</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New""><a class="moz-txt-link-abbreviated" href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mhnc.ch&d=CwMD-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=awiDiMv0NtCLLz5-uNP7bpumXx_y47hSNPzjP1ztGHQ&s=j_kCUhBRpi6St_0mk54mrv-AsRO2bu6fk98EjYXHooQ&e=">www.mhnc.ch</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Courier
New""><o:p> </o:p></span></p>
</div>
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<pre class="moz-signature" cols="72">--
Dirk Neumann
Tel: 089 / 8107-111
Fax: 089 / 8107-300
email: Dirk.Neumann(a)zsm.mwn.de
Postanschrift:
Staatliche Naturwissenschaftliche Sammlungen Bayerns
Zoologische Staatssammlung München
Dirk Neumann, Sektion Ichthyologie / DNA-Labor
Münchhausenstr. 21
81247 München
Besuchen Sie unsere Sammlung:
<a class="moz-txt-link-freetext" href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwMD-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=awiDiMv0NtCLLz5-uNP7bpumXx_y47hSNPzjP1ztGHQ&s=ZAb7gDSyAGwhQGWD5e_OD-UJZa03v9DimjLmwivi2aM&e=">http://www.zsm.mwn.de/ich/</a>
---------
Dirk Neumann
Tel: +49-89-8107-111
Fax: +49-89-8107-300
email: Dirk.Neumann(a)zsm.mwn.de
postal address:
Bavarian Natural History Collections
The Bavarian State Collection of Zoology
Dirk Neumann, Section Ichthyology / DNA-Lab
Muenchhausenstr. 21
81247 Munich (Germany)
Visit our section at:
<a class="moz-txt-link-freetext" href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwMD-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=awiDiMv0NtCLLz5-uNP7bpumXx_y47hSNPzjP1ztGHQ&s=ZAb7gDSyAGwhQGWD5e_OD-UJZa03v9DimjLmwivi2aM&e=">http://www.zsm.mwn.de/ich/</a>
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