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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dirk,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">I vaguely recall a thread from several years ago about this very topic. Someone had done some analysis and talked to the tube manufacturer and their conclusion
was that the silicone gaskets did not seal in ethanol. A search of the nh-coll archives might turn it up as I am clearly a bit vague on the details. They did an assessment of a variety of tubes and recommended the Nalgene ones. We’re using a slightly different
variety than Andy <a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_nalgene-2Dgeneral-2Dlong-2Dterm-2Dstorage-2Dcryogenic-2Dtubes_033377d&d=CwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FuIWHY9LgLat3tvJWJJoITt0mNheD2FcX_K-z7UUpr0&s=-zq1envNU-Pmu6IQnvJpnzx-y3OpkUtkAqBoIgPxvzE&e=">
https://www.fishersci.com/shop/products/nalgene-general-long-term-storage-cryogenic-tubes/033377d</a>. I’m not quite sure what the difference is but I know that what we’re using was the recommendation made at the time.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Best wishes- Jean<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Jean L. Woods, Ph.D. Phone: 302-658-9111 x314<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Curator of Birds Fax: 302-658-2610<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Delaware Museum of Natural History jwoods@delmnh.org<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">P.O. Box 3937 www.delmnh.org<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">(4840 Kennett Pike)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Wilmington, DE 19807<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:windowtext">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:windowtext"> nhcoll-l-bounces@mailman.yale.edu [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Bentley, Andrew Charles<br>
<b>Sent:</b> Thursday, December 01, 2016 2:22 PM<br>
<b>To:</b> Dirk Neumann<br>
<b>Cc:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] dna storage tubes for -80°C<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dirk<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">We have been using these for a number of years now -
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_nalgene-2Dgeneral-2Dlong-2Dterm-2Dstorage-2Dcryogenic-2Dtubes_033377w-3FsearchHijack-3Dtrue-26searchTerm-3D033377W-26searchType-3DRAPID&d=CwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=uQg2Y4uc188QnChKc072r4at7J4xXMUw5btRbaRin8s&e=">
https://www.fishersci.com/shop/products/nalgene-general-long-term-storage-cryogenic-tubes/033377w?searchHijack=true&searchTerm=033377W&searchType=RAPID</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">We specifically selected ungasketed, externally threaded tubes even for -80 freezers which we were using at the time. There were too many reports of gasket
failure at low temps and externally threaded tubes, although taking up slightly more space in storage, negate any contamination issues that may occur by having an internally threaded closure potentially in contact with tissue or preservation fluid.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hope that helps<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Andy<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif""> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif"">Dyche Hall<br>
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Tel: (785) 864-3863<br>
Fax: (785) 864-5335 <br>
Email: <a href="mailto:abentley@ku.edu">abentley@ku.edu</a> <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=CwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=5dr2Rc4jxMQRUmOyMCTo6XtwfwOmwn0Ue_jdOdYQq5U&e=">http://ichthyology.biodiversity.ku.edu</a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif"">SPNHC Past-President<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org_&d=CwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=hQR_d-S8qVfe0uGPRSqZc1MKX2wqXO1vrGKihGZYYCI&e=">http://www.spnhc.org</a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Arial","sans-serif""> : : <br>
A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V</span><span lang="EN-AU" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:windowtext">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:windowtext">
<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a> [<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">mailto:nhcoll-l-bounces@mailman.yale.edu</a>]
<b>On Behalf Of </b>Dirk Neumann<br>
<b>Sent:</b> Thursday, December 01, 2016 9:03 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] dna storage tubes for -80°C<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p>Hi all,<o:p></o:p></p>
<p>I would like to know if there are any specific experiences (good <u>and</u> bad) on DNA-storage tubes (normal freezing as we don't have liquid-N storage).<o:p></o:p></p>
<p>We are using Matrix racks (sepra seals) and I want to change to high quality skrew-lid tubes, but I am sceptical with the silicone gasket in combination with the internal thread of the tubes.<o:p></o:p></p>
<p>Thermo says that the tubes are tight sealing (from liquid-N to normal freezing and even room temperature), but if the gasket starts to crumble, then the tubes necks are open. Also, gaskets are made of silicone, which might cause issues with cold-flowing
behaviour and life span of the material.<o:p></o:p></p>
<p>Curious to hear your thoughts, especially if you have experiences where things turned out to be not that good as originally promised by the manufacturer (also off-line, if you wouldn't like to post any failures to the broad public)<o:p></o:p></p>
<p><o:p> </o:p></p>
<p>Thanks in advance for any helpful feedback<o:p></o:p></p>
<p>Dirk<o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:12.0pt"><o:p> </o:p></p>
<pre>-- <o:p></o:p></pre>
<pre>Dirk Neumann<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Tel: 089 / 8107-111<o:p></o:p></pre>
<pre>Fax: 089 / 8107-300<o:p></o:p></pre>
<pre>email: Dirk.Neumann(a)zsm.mwn.de<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Postanschrift:<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Staatliche Naturwissenschaftliche Sammlungen Bayerns<o:p></o:p></pre>
<pre>Zoologische Staatssammlung München<o:p></o:p></pre>
<pre>Dirk Neumann, Sektion Ichthyologie / DNA-Labor<o:p></o:p></pre>
<pre>Münchhausenstr. 21<o:p></o:p></pre>
<pre>81247 München<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Besuchen Sie unsere Sammlung:<o:p></o:p></pre>
<pre><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwMDaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Zon_pRWu2LgaUZWryH53ux0AYw5xz6rO-vGC5OsQA_c&s=EsDqQFqkkW0MGjC44P59OoWs3ofclT-YiRGrCPksJsU&e=">http://www.zsm.mwn.de/ich/</a><o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>---------<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Dirk Neumann<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Tel: +49-89-8107-111<o:p></o:p></pre>
<pre>Fax: +49-89-8107-300<o:p></o:p></pre>
<pre>email: Dirk.Neumann(a)zsm.mwn.de<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>postal address:<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Bavarian Natural History Collections<o:p></o:p></pre>
<pre>The Bavarian State Collection of Zoology<o:p></o:p></pre>
<pre>Dirk Neumann, Section Ichthyology / DNA-Lab<o:p></o:p></pre>
<pre>Muenchhausenstr. 21<o:p></o:p></pre>
<pre>81247 Munich (Germany)<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>Visit our section at:<o:p></o:p></pre>
<pre><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwMDaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Zon_pRWu2LgaUZWryH53ux0AYw5xz6rO-vGC5OsQA_c&s=EsDqQFqkkW0MGjC44P59OoWs3ofclT-YiRGrCPksJsU&e=">http://www.zsm.mwn.de/ich/</a><o:p></o:p></pre>
<pre><o:p> </o:p></pre>
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