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<div class="moz-cite-prefix">Hi Alexandra,<br>
<br>
I am not sure about the composition of RIR, but if uses
formaldehyde e.g. in the pulp to glue it together, the paper might
be reactive if it gets into contact with formalin, e.g. start to
swell, get sticky / stick together. I once tried a high quality
archival paper and put a small bundle of labels into formalin. As
soon as they entered, they started to swell and stuck firmly
together; it was impossible to detach individual labels from each
other again.<br>
<br>
But there are also other chemicals that could do harm (e.g.
shifting the pH). Normally, if a paper is some sort of water
resistant, either the surface and bottom and/or the pulp is
hydrophobised. Normal paper already uses up to 30 chemicals,
waterproofed paper surely more. Even if you use RIR only
temporarily in the field until samples are returned, you may
already have a chemical cocktail inside because 96% EthOH is a
fairly good organic solvent, too.<br>
<br>
For me, externally pre-numbered tubes seem the safer option; or
tubes that can be marked externally with pencils. If those
internally RIR tagged samples fail during extraction or give bad
sequencing results, you add just one more unknown to the black box
of weird lab mysteries making samples fail.<br>
<br>
I guess the only other option would be to specifically test for
effects of internal RIR tags by subdividing a tissue to two tubes,
one externally labelled, the other with internal RIR, returned
under identical conditions to the lab, and compare e.g. DNA yields
after extraction, PCR results, etc. if you want to be sure. For
sure still not the answer you are looking for, but hope it offers
some sort of help. <br>
<br>
All the best<br>
Dirk<br>
<br>
<br>
Am 04.01.2017 um 21:20 schrieb Alexandra Snyder:<br>
</div>
<blockquote
cite="mid:CAJKsxn2+dCXq82srs8E94n+YuCfVbFeXxEBsgN=pThnodSma5w@mail.gmail.com"
type="cite">
<div dir="ltr">Andy, Absolutely.� No disagreement there, i.e.,
external labels. Thanks for reminding the list.�
<div><br>
</div>
<div>I would still like to document the condition of tissue
accessions rec'd with RIR labels and the USFWS hatchery folks
are curious re: the use of Rite in Rain for temporary labels.�</div>
<div><br>
</div>
<div>If there is a way to get an idea or if anyone has knowledge
of RIR properties, I would like to hear about it.� I recognize
that manufacturers do not share recipes, �but one never knows.<br>
</div>
<div><br>
</div>
<div>Lex</div>
</div>
<div class="gmail_extra"><br>
<div class="gmail_quote">On Wed, Jan 4, 2017 at 11:41 AM,
Bentley, Andrew Charles <span dir="ltr"><<a moz-do-not-send="true" href="mailto:abentley@ku.edu" target="_blank">abentley@ku.edu</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0
.8ex;border-left:1px #ccc solid;padding-left:1ex">
<div bgcolor="white" link="#0563C1" vlink="#954F72"
lang="EN-US">
<div class="m_-2981454734265102888WordSection1">
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:Calibri">Lex</span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:Calibri">�</span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:Calibri">We have
a policy here at KU that nothing gets put inside the
tube except the tissue and preservative (if
necessary).� There are just too many possible
avenues of contamination from the �paper� to the
�ink� to the residue left on the paper by human
contact.� All labels are external and usually
adhered before going in to the field using a Brady
labeling system and cryo safe labeling material.</span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:Calibri">�</span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:Calibri">Andy</span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:Calibri">�</span></p>
<div>
<div>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">�
� A� :������������ A� :������������ A� :</span></p>
</div>
<div>
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style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">�}<(((_�>.,.,.,.}<(((_�>.,.,.,<wbr>.}<)))_�></span></p>
</div>
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style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">���
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</div>
<div>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">Andy
Bentley</span></p>
</div>
<div>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">Ichthyology
Collection Manager</span></p>
</div>
<div>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">University
of Kansas</span></p>
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style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">Biodiversity
Institute</span></p>
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style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">Lawrence,
KS, 66045-7561</span></p>
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<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">USA</span></p>
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<div>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">�</span></p>
</div>
<div>
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style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">Tel:
<a moz-do-not-send="true" href="tel:%28785%29%20864-3863" value="+17858643863" target="_blank">(785)
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<a moz-do-not-send="true" href="tel:%28785%29%20864-5335" value="+17858645335" target="_blank">(785)
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<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">Email:�<a moz-do-not-send="true" href="mailto:abentley@ku.edu" target="_blank"><span
style="color:blue">abentley@ku.edu</span></a>��</span></p>
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<p class="MsoNormal"><span
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President</span></p>
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<p class="MsoNormal"><span
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<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">��������������������������
:���������������� :����</span></p>
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style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">���
A� :������������ A� :������������ A� :</span></p>
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<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">�}<(((_�>.,.,.,.}<(((_�>.,.,.,<wbr>.}<)))_�></span></p>
</div>
</div>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:"-webkit-standard","serif";color:black">���
V��������������� V��������������� V</span><span
style="font-size:11.0pt;font-family:Calibri"></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:Calibri">�</span></p>
<div style="border:none;border-top:solid #b5c4df
1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span
style="font-family:Calibri;color:black">From: </span>
</b><span style="font-family:Calibri;color:black"><<a moz-do-not-send="true" href="mailto:nhcoll-l-bounces@mailman.yale.edu" target="_blank">nhcoll-l-bounces@mailman.<wbr>yale.edu</a>>
on behalf of Alexandra Snyder <<a moz-do-not-send="true" href="mailto:amsnyder@unm.edu" target="_blank">amsnyder@unm.edu</a>><br>
<b>Date: </b>Wednesday, January 4, 2017 at 12:10
PM<br>
<b>To: </b>"<a moz-do-not-send="true" href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a>"
<<a moz-do-not-send="true" href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a>><br>
<b>Subject: </b>[Nhcoll-l] Rite-in-Rain Paper</span></p>
</div>
<div>
<p class="MsoNormal">�</p>
</div>
<div id="m_-2981454734265102888divtagdefaultwrapper">
<p><span style="font-family:Calibri;color:black">Rite-in-Rain
or "all weather" field paper is used by the many
agency�biologists depositing specimens and genetic
samples with the MSB Division of Fishes and other
repositories. � Recently, the USFWS Southwestern
Native Aquatic Resources and Recovery Center has
requested that I find out if there are any issues
with using this paper for genetic labels in the
field or otherwise.� They tried to get information
from the Rite-in-Rain company re: what was in the
paper and coating (formalin?) to determine if the
paper would pose a problem for genetic samples.�
The company was not forthcoming re: Rite-in-Rain
product specifics. �</span></p>
<p><span style="font-family:Calibri;color:black">�</span></p>
<p><span style="font-family:Calibri;color:black">We
have had many genetic�samples received at both the
Center and the MSB with these�paper tags �made by
tearing pieces of paper out of�the RIR�field
books, writing the sample number on RIR�tag with
pencil, and�stuffing the tag into a�cryotube.� Not
optimal in terms of contamination issues, etc. but
getting word out to field crews, etc. can be
difficult.� For many projects, I�preprint labels
on polyspun paper (DataMax)�for researchers,�but
there are many opportunistic�collections that
receive these temporary labels.�</span></p>
<p><span style="font-family:Calibri;color:black">�</span></p>
<p><span style="font-family:Calibri;color:black">Any
thoughts or insights?� Does anyone have an idea of
how this paper is manufactured/treated?</span></p>
<p><span style="font-family:Calibri;color:black">�Thank
you.</span></p>
<div id="m_-2981454734265102888Signature">
<div id="m_-2981454734265102888divtagdefaultwrapper">
<p style="background:white"><span
style="font-family:Calibri;color:black">�</span></p>
<div name="divtagdefaultwrapper">
<div>
<div>
<div>
<p class="MsoNormal"
style="background:white"><span
style="color:black">******************************<wbr>*******</span><span
style="font-size:10.0pt;font-family:Tahoma;color:black"></span></p>
</div>
<div>
<p class="MsoNormal"
style="background:white"><span
style="font-size:13.5pt;color:gray">Alexandra
M Snyder, Collections Manager-Fishes</span><span
style="font-size:10.0pt;font-family:Tahoma;color:black"></span></p>
</div>
<div>
<p class="MsoNormal"
style="background:white"><span
style="color:gray">Museum of
Southwestern Biology MSC03-2020</span><span
style="font-size:10.0pt;font-family:Tahoma;color:black"></span></p>
</div>
<div>
<p class="MsoNormal"
style="background:white"><span
style="color:gray">University of New
Mexico Albuquerque NM 87131</span><span
style="font-size:10.0pt;font-family:Tahoma;color:black"></span></p>
</div>
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style="color:gray">PH./FAX <a moz-do-not-send="true" href="tel:%28505%29%20277-6005" value="+15052776005" target="_blank">505.277.6005</a>
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style="background:white"><span
style="font-size:10.0pt;font-family:Tahoma;color:black">�</span></p>
</div>
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<p class="MsoNormal"
style="background:white"><span
style="color:gray">PHYSICAL ADDRESS
FOR FEDEX/UPS</span><span
style="font-size:10.0pt;font-family:Tahoma;color:black"></span></p>
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style="font-size:10.0pt;font-family:Tahoma;color:black">�</span></p>
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</blockquote>
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<br>
<br clear="all">
<div><br>
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-- <br>
<div class="gmail_signature" data-smartmail="gmail_signature">
<div dir="ltr">
<div>
<div dir="ltr">
<div dir="ltr">
<div>*****************************************************</div>
<div>Alexandra M Snyder</div>
<div>Collections Manager-Fishes<br>
</div>
Museum of Southwestern Biology�<span
style="font-size:12.8px">MSC01-2020�</span></div>
<div dir="ltr">
<div style="font-size:12.8px">University of New Mexico</div>
<div style="font-size:12.8px">Albuquerque NM 87131 USA</div>
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address for FedEx and UPS</u></span><br>
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<pre wrap="">_______________________________________________
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<pre class="moz-signature" cols="72">--
Dirk Neumann
Tel: 089 / 8107-111
Fax: 089 / 8107-300
email: Dirk.Neumann(a)zsm.mwn.de
Postanschrift:
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