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<div class="moz-cite-prefix">Good morning Kareen & Rachael,<br>
<br>
I am not sure if there is first hand experience how mercuric
chloride behaves exactly, but it was used as fixative especially
for marine invertebrates in the early 20th century (sublimate; cf.
Piechocki & H�ndel, 2007. Makroskopische Pr�parationstechnik,
Wirbellose, 5th Edition) and as early antibiotic (e.g. against
syphilis) because of its toxic properties. So I would assume that
it does interact with tissues and DNAs and consequently do
influence sequencing results.<br>
<br>
Perhaps Joachim can add more details ?<br>
<br>
Hope this helps<br>
Dirk<br>
<br>
Am 23.01.2017 um 23:54 schrieb Kareen Schnabel:<br>
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<p class="MsoNormal"><span
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morning all, I thought this listserv may have some valuable
input into my colleague�s query to the Crustacean listserv.
Please see query below and please reply to Rachael Peart <a moz-do-not-send="true" href="mailto:rachaelapeart@gmail.com">
rachaelapeart@gmail.com</a>.<b><o:p></o:p></b></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Thanks<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Kareen<o:p></o:p></span></p>
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</font><font
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Schnabel</font></td>
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<p class="MsoNormal"><b><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif"
lang="EN-US">From:</span></b><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif"
lang="EN-US"> <a class="moz-txt-link-abbreviated" href="mailto:crust-l-request@vims.edu">crust-l-request@vims.edu</a>
[<a class="moz-txt-link-freetext" href="mailto:crust-l-request@vims.edu">mailto:crust-l-request@vims.edu</a>]
<b>On Behalf Of </b>Rachael Peart<br>
<b>Sent:</b> Tuesday, 24 January 2017 11:49 a.m.<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:crust-l@vims.edu">crust-l@vims.edu</a><br>
<b>Subject:</b> [CRUST-L:9947] Mercuric chloride and
sequencing<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p>�</o:p></p>
<div style="border:none;border-top:solid white
1.0pt;padding:3.0pt 0cm 0cm 0cm">
<div>
<div>
<p class="MsoNormal">Hi all,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">I have come across some amphipod
samples that were captured in traps using mercuric
chloride.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">I am curious to know if this chemical
can affect molecular sequencing - ie COI or 18S?<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Does it completely destroy the
amounts, or do you get traces, or no damage?<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p>�</o:p></p>
</div>
<div>
<p class="MsoNormal">Thanks in advance<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p>�</o:p></p>
</div>
<div>
<p class="MsoNormal">Rachael Peart<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p>�</o:p></p>
</div>
</div>
<div>
<p class="MsoNormal"><o:p>�</o:p></p>
</div>
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