<div dir="ltr">Hi Rachael,<div><br></div><div>these are both quite old references and contemporary techniques can yield usable data from much more fragmented DNA than was possible back in 2003. I expect the High Throughput Sequencing techniques being used for formalin treated material my be applicable to specimens preserved in mercuric chloride: <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__journals.plos.org_plosone_article-3Fid-3D10.1371_journal.pone.0141579&d=CwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=7Hv0d9U59ARm-uJ3xwzPGUpdSBFvTyKM0ZHs1Gr8kUQ&s=wfyTfwZHLSNzoV9KY6fhmWfvpjFkXDgHSIRmQKVcMmA&e=">http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141579</a> </div><div><br></div><div>Cheers,</div><div><br></div><div>Paolo</div></div><div class="gmail_extra"><br><div class="gmail_quote">On 24 January 2017 at 20:14, Rachael Peart <span dir="ltr"><<a href="mailto:rachaelapeart@gmail.com" target="_blank">rachaelapeart@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr"><div>Thanks for the discussion,</div><div>It looks like due to it's toxic nature, there is no extraction possible from animals preserved with mercuric chloride based products.</div><div>Found these articles.</div><div>O'Leary et al, 1994 - "The importance of fixation procedures on DNA template and its suitability for solution-phase polymerase chain reaction and PCR <em>in situ </em>hybridisation."</div><div>The Histochemical Journal 26 (4): 337 - 346.</div><div>C. Schander and K.M. Halanych, 2003. "DNA, PCR and formalinized animal tissue - a short review and protocols." Organisms Diversity and Evolution 3 (3): 195 - 205.</div><div><br></div><div>Thanks anyway</div><span class="HOEnZb"><font color="#888888"><div><br></div><div>Rachael</div></font></span></div><div class="HOEnZb"><div class="h5"><div class="gmail_extra"><br><div class="gmail_quote">On Tue, Jan 24, 2017 at 9:50 PM, Joachim Haendel <span dir="ltr"><<a href="mailto:joachim.haendel@zns.uni-halle.de" target="_blank">joachim.haendel@zns.uni-<wbr>halle.de</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hello Kareen & Rachael,<br>
and hello Dirk,<br>
<br>
well - this is really interesting. But I can't say anything about this. To my knowledge there are no researches<br>
on this topic. Mercury(II) chloride is an effective antiseptic and disinfectant and it is corrosive. It is thinkable<br>
that it will damage the DNA. Maybe someone could try it out?! I think many museo-biologists want to know<br>
the results.<br>
<br>
Greetings<br>
Joachim<br>
<br>
<br>
<br>
---- On 24 Jan 2017 at 8:36, Dirk Neumann wrote:<br>
<br>
> Good morning Kareen & Rachael,<br>
><br>
> I am not sure if there is first hand experience how mercuric chloride<br>
> behaves exactly, but it was used as fixative especially for marine<br>
> invertebrates in the early 20th century (sublimate; cf. Piechocki &<br>
> Händel, 2007. Makroskopische Präparationstechnik, Wirbellose, 5th<br>
> Edition) and as early antibiotic (e.g. against syphilis) because of its<br>
> toxic properties. So I would assume that it does interact with tissues<br>
> and DNAs and consequently do influence sequencing results.<br>
><br>
> Perhaps Joachim can add more details ?<br>
><br>
> Hope this helps<br>
> Dirk<br>
><br>
> Am 23.01.2017 um 23:54 schrieb Kareen Schnabel:<br>
> ><br>
> > Good morning all, I thought this listserv may have some valuable input<br>
> > into my colleague´s query to the Crustacean listserv. Please see query<br>
> > below and please reply to Rachael Peart <a href="mailto:rachaelapeart@gmail.com" target="_blank">rachaelapeart@gmail.com</a><br>
> > <mailto:<a href="mailto:rachaelapeart@gmail.com" target="_blank">rachaelapeart@gmail.co<wbr>m</a>>.**<br>
> ><br>
> > Thanks<br>
> ><br>
> > Kareen<br>
> ><br>
> ><br>
> > DrKareen Schnabel<br>
> > Marine Biologist<br>
> ><br>
> > *From:*<a href="mailto:crust-l-request@vims.edu" target="_blank">crust-l-request@vims.ed<wbr>u</a> [mailto:<a href="mailto:crust-l-request@vims.edu" target="_blank">crust-l-request@vims.e<wbr>du</a>] *On<br>
> > Behalf Of *Rachael Peart<br>
> > *Sent:* Tuesday, 24 January 2017 11:49 a.m.<br>
> > *To:* <a href="mailto:crust-l@vims.edu" target="_blank">crust-l@vims.edu</a><br>
> > *Subject:* [CRUST-L:9947] Mercuric chloride and sequencing<br>
<div><div class="m_8059260464337643679h5">> ><br>
> > Hi all,<br>
> ><br>
> > I have come across some amphipod samples that were captured in traps<br>
> > using mercuric chloride.<br>
> ><br>
> > I am curious to know if this chemical can affect molecular sequencing<br>
> > - ie COI or 18S?<br>
> ><br>
> > Does it completely destroy the amounts, or do you get traces, or no<br>
> > damage?<br>
> ><br>
> > Thanks in advance<br>
> ><br>
> > Rachael Peart<br>
> ><br>
> ><br>
<br>
<br>
<br>
</div></div>--<br>
Joachim Haendel<br>
Center of Natural Sciences Collections<br>
of the Martin-Luther-University<br>
- Entomological Collection -<br>
<br>
Domplatz 4<br>
D-06099 Halle (Saale)<br>
Germany<br>
<br>
Phone: <a href="tel:%2B49%20345%20-%2055%2026%20447" value="+493455526447" target="_blank">+49 345 - 55 26 447</a><br>
Fax: <a href="tel:%2B49%20345%20-%2055%2027%20248" value="+493455527248" target="_blank">+49 345 - 55 27 248</a><br>
Email: <a href="mailto:joachim.haendel@zns.uni-halle.de" target="_blank">joachim.haendel@zns.uni-halle.<wbr>de</a><br>
<br>
<br>
</blockquote></div><br></div>
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