<div dir="ltr"><b><i>Just so no one is concerned, </i></b>we have a protocol in place that has received BioSafety clearance to perform the macerations in an appropriate facility without formalin fixation. We were just considering formalin fixation as an alternative.<div class="gmail_extra"><br><div class="gmail_quote">On Fri, May 26, 2017 at 11:49 AM, David Katz <span dir="ltr"><<a href="mailto:dckatz@ucdavis.edu" target="_blank">dckatz@ucdavis.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">Thanks everyone. We will stick with out original (non-formalin) plan. The reason for considering formalin is because these animals are potentially disease vectors. By "short" I meant fixation for a sufficient length of time that the tissue would be fixed. I was contrasting it with a situation where you might have an animal sitting in formalin for years (sometimes happens in anatomy classes for dissection specimens that don't get used).<br><div><br></div><div>Really appreciate the help. </div><span class="m_-6568396642212282050m_2338553769586607757HOEnZb"><font color="#888888"><div><br>David</div></font></span></div><div class="m_-6568396642212282050m_2338553769586607757HOEnZb"><div class="m_-6568396642212282050m_2338553769586607757h5"><div class="gmail_extra"><br><div class="gmail_quote">On Fri, May 26, 2017 at 1:59 AM, Dirk Neumann <span dir="ltr"><<a href="mailto:dirk.neumann@zsm.mwn.de" target="_blank">dirk.neumann@zsm.mwn.de</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div text="#000000" bgcolor="#FFFFFF">
<div class="m_-6568396642212282050m_2338553769586607757m_-7591564176993726354m_-5483477517927536125moz-cite-prefix">Dear David,<br>
<br>
because of the low pH formaldehyde decalcifies the the bones and
can make (tiny) bones brittle. Also, because of the covalent
bonding inside tissues, enzymatic degradation of tissues is
hindered. Unbound formalin escaping during maceration from
specimens could cause formaldehyde gas to get trapped under the
lid of the maceration tank and might cause health issues when
opening the tank.<br>
<br>
I am also not sure if "short" fixation would result in sufficient
penetration into tissues, so you would need to expose the bodied
for sufficient long time (depending on the size and amount of
tissue) <br>
<br>
This would be the immediate concerns that jump into my mind, but
there might be more.<br>
<br>
Hope this helps<br>
Dirk<div><div class="m_-6568396642212282050m_2338553769586607757m_-7591564176993726354h5"><br>
<br>
<br>
Am 26.05.2017 um 03:45 schrieb David Katz:<br>
</div></div></div>
<blockquote type="cite"><div><div class="m_-6568396642212282050m_2338553769586607757m_-7591564176993726354h5">
<div dir="ltr">Hi everyone,
<div><br>
</div>
<div>We will have a number of medium bodied primate carcasses
that we plan to macerate in order to preserve the skeletons.
We've been told that fixing with formaldehyde prior to
maceration can negatively affect the bones. </div>
<div><br>
</div>
<div>What are the consequences? Is this an issue for both short
and long duration fixation, or just long? Thanks very much.</div>
<div><br>
David<br clear="all">
<div><br>
</div>
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<div>
<div dir="ltr">
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<div dir="ltr">
<div dir="ltr">
<div>David C. Katz, Ph.D.</div>
<div>
<div style="font-size:12.8px">Postdoctoral
Fellow</div>
<div style="font-size:12.8px">Benedikt
Hallgrimsson L<span style="font-size:12.8px">ab</span></div>
<div style="font-size:12.8px">University of
Calgary</div>
<div style="font-size:12.8px"><br>
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