<div dir="ltr"><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:rgb(0,0,0)">Juliette,<br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:rgb(0,0,0)">You have asked a very interesting question. I agree with the responses from my colleagues concerning the caution about osmotic pressure and the kinds of specimens involved. Could you please tell us (1) what kinds of specimens are in the collection (marine invertebrates or vertebrates, fresh water or terrestrial invertebrates or vertebrates?) and (2) the reason why you are considering changing the preserving to Kaiserling? Kaiserling has been mostly used for anatomical specimens in an attempt to preserve the color of the tissues, and as far as I know it has not been used on a very wide variety of natural history specimens.<br><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:rgb(0,0,0)">Sincerely,<br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:rgb(0,0,0)">John<br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:rgb(0,0,0)"><br></div></div><div class="gmail_extra"><br clear="all"><div><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr">John E. Simmons<br>Museologica<br>128 E. Burnside Street<br>Bellefonte, Pennsylvania 16823-2010<br><a href="mailto:simmons.johne@gmail.com" target="_blank">simmons.johne@gmail.com</a><br>303-681-5708<br><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.museologica.com&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=fSD02RfLBQBnZOWZoZoo8rqjhicine8IXA0tOZwONDU&s=9cktIx78txyeohVggLZ_3XuGQ8f6dAcW3Pk3HvymSOk&e=" target="_blank">www.museologica.com</a><br>and<br>Adjunct Curator of Collections<br>Earth and Mineral Science Museum & Art Gallery<br>Penn State University<br>University Park, Pennsylvania<br>and<br>Instructor, Museum Studies<br>School of Library and Information Science<br>Kent State University<br></div></div></div></div></div>
<br><div class="gmail_quote">On Wed, Aug 16, 2017 at 9:09 AM, Dirk Neumann <span dir="ltr"><<a href="mailto:dirk.neumann@zsm.mwn.de" target="_blank">dirk.neumann@zsm.mwn.de</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div text="#000000" bgcolor="#FFFFFF">
<div class="m_-2817061451362062073moz-cite-prefix">Dear Simon,<br>
dear Galpin,<br>
<br>
the baseline for changing the holding fluid is: Do not change,
unless there is a good reason to do so.<br>
<br>
From a collection care point of view, fire protection or toxicity
problems - for me - would not be a proper reason if in the long
run the new condition would harm the integrity of the specimen. <br>
<br>
Also, because of the acidity, I would refrain of changing the
fluid unless there is a real good reason to do so (maybe part of
the collection, or specific specimens from a lot containing
several specimens for a specific research purpose).<br>
<br>
Btw:<br>
Mahoney (1973) Laboratory Techniques in Zoology gives the
following recipes (Pick-Judah formula)<br>
(Simon, please comment if this wouldn't make sense)<br>
<br>
KI (fixative)<br>
Potassium acetate 42.5g<br>
Potassium nitrate 22.5g<br>
Distilled water 1.6 L<br>
40% formalin solution 400ml (full strength)<br>
<br>
Salt are dissolved in hot water, formalin is added to the cooled
solution<br>
<br>
KII (colour reviver)<br>
EtOH 96 %<br>
<br>
KIII (perservative)<br>
Sodium acetate 36.0g<br>
Distilled water 120.0ml<br>
Camphor 5.0ml (4% EtOH solution)<br>
Glycerol 72ml<br>
<br>
(The solution is made by adding the ingredients in the order
given. At first, the camphor is thrown out of solution but
redissolves on allowing the solution to stand)<br>
<br>
All the best<br>
Dirk<div><div class="h5"><br>
<br>
<br>
Am 16.08.2017 um 13:19 schrieb Simon Moore:<br>
</div></div></div>
<blockquote type="cite"><div><div class="h5">Hi
Juliette,
<div><br>
</div>
<div>No problem about getting in touch.</div>
<div><br>
</div>
<div>You mention re-packing a collection. Is this
collection to be moved or stored or did you mean that is needs
to be moved into safer fluids? The latter is indicative of a
move for museums with such collections to be rid of fluids that
are flammable and/or toxic.</div>
<div><br>
</div>
<div>There are various concerns about this as the
condition of the specimens is what should be the real issue
here. As you are based at LeHavre I am assuming that you should
have many marine organisms in fluids? For many such organisms a
move to Kaiserling III will be fine but you have to bear in mind
that the osmotic pressure of KIII is considerably different to
that of ethanol, even formalin. For delicate organisms
(medusae, siphonophores) this changeover has to be carried out
very carefully or you will end up with air bubbles inside them
that appear when binary/tertiary azeotropes are used - miscible
fluids of different specific gravities. You also need to bear
in mind that Kaiserling’s technique was devised mainly for
anatomy specimens as the preservative is good for maintaining
the colour of blood and organs that filter it (liver, kidney).
I have successfully colour-preserved marine organisms that were
freshly collected and using the 3 stage technique - fixation,
colour enhancement and preservation.</div>
<div>All of this may sound rather negative but I am just
warning you to be careful with the changeover. I would also
post the enquiry onto the conservation forum: <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a> to see if
anyone else is doing this and to see if they have any concerns.</div>
<div><br>
</div>
<div>The formula for the Kaiserling method is: <span style="font-family:'Bookman Old Style';font-size:9pt;text-align:justify">Kaiserling
I (fixative): 40% formaldehyde (400) [tlv 3], potassium
acetate (50 g), potassium
nitrate (30 g), distilled water (1000).</span></div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Bookman Old Style"" lang="EN-GB">Kaiserling II (enhancer/colour restorer):
80-90% ethanol. Use until colour returns if it has chemically
‘faded’ in
fixative – may be minutes for smaller specimens. Some like to
add some potassium
hydrosulphite if being stored for more than a few days.<u></u><u></u></span></p>
<p class="MsoNormal" style="margin-right:7.2pt;text-align:justify"><span lang="EN-GB">Kaiserling
III (preservative): Potassium acetate (200g), glycerol (300),
distilled water
(900).<u></u><u></u></span></p>
<div><br>
</div>
<div>
<div>
<div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word">
<div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word">
<div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word">With
all good wishes, Simon.</div>
<div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word"><br>
</div>
<div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word">Simon
Moore MIScT, RSci, FLS, ACR<br>
Conservator of Natural Sciences and Cutlery Historian,<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=MxsNB9i59J38j_fgIMI_hJkw5F2sMUmH0Nl7mDt2jiY&s=Q68m7UHxI0JOb8NE7fTQh74O6YrzMiczUotWcxiwwDk&e=" target="_blank">www.natural-history-<wbr>conservation.com</a> </div>
<div style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;word-wrap:break-word"><br>
<br>
<br>
</div>
</div>
</div>
</div>
<br>
<div>
<blockquote type="cite">
<div>On 16 Aug 2017, at 10:37, Galpin Juliette <<a href="mailto:juliette.galpin@lehavre.fr" target="_blank">juliette.galpin@lehavre.fr</a>>
wrote:</div>
<br class="m_-2817061451362062073Apple-interchange-newline">
<div>
<div class="m_-2817061451362062073WordSection1" style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px">
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">Hello,<span class="m_-2817061451362062073Apple-converted-space"> </span><u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US"><u></u> <u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">My name is Juliette Galpin and
I’m in charge of all the scientific collection in
the Natural History Museum of Le Havre, in France.<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">I’m following the advice of
Judith White, in Natural History Museum, who told me
to get in touch with you. Hope you won’t mind.<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US"><u></u> <u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">We have a collection which is
maintained in fluids, and we want to repack it. The
specimens are currently in liquid as Alcool, Ethanol
and a bit of Formol. And we wish to recondition it
in Kaiserling III, which is a mixture of glycerin
and acetate of potassium without any formol. I don’t
know if you know, or use, this liquid.<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">I’ve found some of your papers
about collections in fluids, but there is few
literature about Kaiserling precisely, and I don’t
really know how to create this liquid correctly.<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">Do you know this product, or
someone who might know and use it, or maybe know the
process to adapt the specimens to their new
conservation fluid ?<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US"><u></u> <u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">Thank you very much for your
answer and all the help you can provide me,<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US"><u></u> <u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">Regards.<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-US">Juliette Galpin<u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span><span id="m_-2817061451362062073cid:image001.jpg@01D31684.16BFD080"><image001.jpg></span></span><span><u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><b><span style="font-size:7.5pt;font-family:Arial,sans-serif" lang="EN-US">Juliette
GALPIN</span></b><span style="font-size:7.5pt;font-family:Arial,sans-serif" lang="EN-US"><br>
In charge of Natural history Collections</span><span lang="EN-US"><u></u><u></u></span></div>
<div style="margin:0cm 0cm 0.0001pt;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:7.5pt;font-family:Arial,sans-serif">Natural history Museum<br>
Ville du Havre<br>
<a href="mailto:juliette.galpin@lehavre.fr" style="color:purple;text-decoration:underline" target="_blank">juliette.galpin@lehavre.fr</a><br>
Tél. : 02 35 54 75 89 / 02.32.74.79.90</span></div>
</div>
</div>
</blockquote>
</div>
<br>
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