<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Juliette,<div class=""><br class=""></div><div class="">No problem about getting in touch.</div><div class=""><br class=""></div><div class="">You mention re-packing a collection. Is this collection to be moved or stored or did you mean that is needs to be moved into safer fluids? The latter is indicative of a move for museums with such collections to be rid of fluids that are flammable and/or toxic.</div><div class=""><br class=""></div><div class="">There are various concerns about this as the condition of the specimens is what should be the real issue here. As you are based at LeHavre I am assuming that you should have many marine organisms in fluids? For many such organisms a move to Kaiserling III will be fine but you have to bear in mind that the osmotic pressure of KIII is considerably different to that of ethanol, even formalin. For delicate organisms (medusae, siphonophores) this changeover has to be carried out very carefully or you will end up with air bubbles inside them that appear when binary/tertiary azeotropes are used - miscible fluids of different specific gravities. You also need to bear in mind that Kaiserling’s technique was devised mainly for anatomy specimens as the preservative is good for maintaining the colour of blood and organs that filter it (liver, kidney). I have successfully colour-preserved marine organisms that were freshly collected and using the 3 stage technique - fixation, colour enhancement and preservation.</div><div class="">All of this may sound rather negative but I am just warning you to be careful with the changeover. I would also post the enquiry onto the conservation forum: <a href="mailto:nhcoll-l@mailman.yale.edu" class="">nhcoll-l@mailman.yale.edu</a> to see if anyone else is doing this and to see if they have any concerns.</div><div class=""><br class=""></div><div class="">The formula for the Kaiserling method is: <span style="font-family: 'Bookman Old Style'; font-size: 9pt; text-align: justify;" class="">Kaiserling
I (fixative): 40% formaldehyde (400) [tlv 3], potassium acetate (50 g), potassium
nitrate (30 g), distilled water (1000).</span></div>
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<!--StartFragment--><p class="MsoNormal"><span lang="EN-GB" style="font-size:9.0pt;mso-bidi-font-size:
12.0pt;font-family:"Bookman Old Style"" class="">Kaiserling II (enhancer/colour restorer):
80-90% ethanol. Use until colour returns if it has chemically ‘faded’ in
fixative – may be minutes for smaller specimens. Some like to add some potassium
hydrosulphite if being stored for more than a few days.<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin-right:7.2pt;text-align:justify"><span lang="EN-GB" style="font-size:9.0pt;mso-bidi-font-size:12.0pt;font-family:"Bookman Old Style"" class="">Kaiserling
III (preservative): Potassium acetate (200g), glycerol (300), distilled water
(900).<o:p class=""></o:p></span></p>
<!--EndFragment--><div class=""><br class=""></div><div class=""><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">With all good wishes, Simon.</div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><br class=""></div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Simon Moore MIScT, RSci, FLS, ACR<br class="">Conservator of Natural Sciences and Cutlery Historian,<br class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=MxsNB9i59J38j_fgIMI_hJkw5F2sMUmH0Nl7mDt2jiY&s=Q68m7UHxI0JOb8NE7fTQh74O6YrzMiczUotWcxiwwDk&e=" class="">www.natural-history-conservation.com</a> </div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><br class=""><br class=""><br class=""></div></div></div>
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<br class=""><div><blockquote type="cite" class=""><div class="">On 16 Aug 2017, at 10:37, Galpin Juliette <<a href="mailto:juliette.galpin@lehavre.fr" class="">juliette.galpin@lehavre.fr</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">Hello,<span class="Apple-converted-space"> </span><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">My name is Juliette Galpin and I’m in charge of all the scientific collection in the Natural History Museum of Le Havre, in France.<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">I’m following the advice of Judith White, in Natural History Museum, who told me to get in touch with you. Hope you won’t mind.<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">We have a collection which is maintained in fluids, and we want to repack it. The specimens are currently in liquid as Alcool, Ethanol and a bit of Formol. And we wish to recondition it in Kaiserling III, which is a mixture of glycerin and acetate of potassium without any formol. I don’t know if you know, or use, this liquid.<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">I’ve found some of your papers about collections in fluids, but there is few literature about Kaiserling precisely, and I don’t really know how to create this liquid correctly.<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">Do you know this product, or someone who might know and use it, or maybe know the process to adapt the specimens to their new conservation fluid ?<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">Thank you very much for your answer and all the help you can provide me,<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">Regards.<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">Juliette Galpin<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span id="cid:image001.jpg@01D31684.16BFD080"><image001.jpg></span></span><span style="font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span lang="EN-US" style="font-size: 7.5pt; font-family: Arial, sans-serif;" class="">Juliette GALPIN</span></b><span lang="EN-US" style="font-size: 7.5pt; font-family: Arial, sans-serif;" class=""><br class="">In charge of Natural history Collections</span><span lang="EN-US" style="font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 7.5pt; font-family: Arial, sans-serif;" class="">Natural history Museum<br class="">Ville du Havre<br class=""><a href="mailto:juliette.galpin@lehavre.fr" style="color: purple; text-decoration: underline;" class="">juliette.galpin@lehavre.fr</a><br class="">Tél. : 02 35 54 75 89 / 02.32.74.79.90</span></div></div></div></blockquote></div><br class=""></div></body></html>