<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Perry,<div class=""><br class=""></div><div class="">Glad (in a way!) that the turbidity test proved to be positive as it indicates that you have a large amount of lipid in solution as well as (probably) coating the specimen and this bears out the reason for the pH lowering.</div><div class=""><br class=""></div><div class="">The glitter is odd. Have there been (or are there still) small pebbles in the display tank? Sometimes designers will add these to improve the appearance of the specimen and these often contain small bits of glitter, rather like gneiss with mica.</div><div class=""><br class=""></div><div class="">The issue with the 5% formalin versus alcohol - I still stand by what I said although I should have added: provided that it is well maintained! Always a problem these days with thinner collection staffing levels. </div><div class=""><br class=""><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">With all good wishes, Simon.</div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><br class=""></div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Simon Moore MIScT, RSci, FLS, ACR<br class="">Conservator of Natural Sciences and Cutlery Historian,<br class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=1AeqtGsSEHoyN19MUD9LUvIHlntcw_PUKTDhYs658Ss&s=mOBvxcjuLzRR1j6QaahcIFHKi6pqWelFdRgB4uFLSHs&e=" class="">www.natural-history-conservation.com</a> </div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><br class=""><br class=""><br class=""></div></div></div>
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<br class=""><div><blockquote type="cite" class=""><div class="">On 14 Sep 2017, at 00:27, Perry Poon <<a href="mailto:Perry.Poon@ocean.org" class="">Perry.Poon@ocean.org</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Hi Simon<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">I did the turbidity test of the coelacanth display alcohol. I put a sample of the alcohol into a petri dish and the liquid became white and cloudy.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">I saw several small droplets of fat in the liquid and a thin film of fat that covered most of the petri dish on the liquid’s surface.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">I was told by a colleague that there are small pieces of glitter on the surface of the Coelacanth’s skin that are less than a millimeter in size. They are very minute, and I see them when the light hits them at a particular angle.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Have you ever seen these ? What are these?<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Thank you for all your help and expertise.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Cheers,<o:p class=""></o:p></span></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class=""><span style="font-family: Calibri, sans-serif;" class="">Perry Poon</span></b><span style="font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><span class="Apple-converted-space"> </span><br class=""></span><span style="font-size: 10pt; font-family: Calibri, sans-serif; text-transform: uppercase;" class="">COORDINATOR, PROPS</span><span style="color: rgb(31, 73, 125);" class=""><o:p class=""></o:p></span></div><p class="MsoNormal" style="margin: 3pt 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="font-family: Calibri, sans-serif; color: rgb(0, 94, 184);" class=""><a href="mailto:Perry.Poon@ocean.org" title="Click to send email to Perry Poon" style="color: purple; text-decoration: underline;" class=""><span style="color: rgb(0, 94, 184);" class="">Perry.Poon@ocean.org</span></a></span><span style="font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><span class="Apple-converted-space"> </span><br class="">D 604 659 3489 </span><span style="color: rgb(31, 73, 125);" class=""><o:p class=""></o:p></span></p><div class="MsoNormal" style="margin: 0in 0in 0.0001pt; 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font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">From:</span></b><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="Apple-converted-space"> </span>Simon Moore [<a href="mailto:couteaufin@btinternet.com" class="">mailto:couteaufin@btinternet.com</a>]<span class="Apple-converted-space"> </span><br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>Tuesday, September 12, 2017 3:27 PM<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span>Perry Poon <<a href="mailto:Perry.Poon@ocean.org" class="">Perry.Poon@ocean.org</a>><br class=""><b class="">Cc:</b><span class="Apple-converted-space"> </span>John E Simmons <<a href="mailto:simmons.johne@gmail.com" class="">simmons.johne@gmail.com</a>><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>Re: [Nhcoll-l] Coelacanth deterioration<o:p class=""></o:p></span></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Hi Perry,<o:p class=""></o:p></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">At pH 4 the skeleton and other calcium salt-containing areas start to decalcify - effectively removing the inorganic matrix. This leads to scale drop as well as weakening of the skeleton and eventual specimen collapse.<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Try a simple turbidity test as well. A Petri dish on a black background with some water init - pipette some of the alcohol from the specimen - is their any cloudiness or turbidity at all? If so, the this would represent saturation of lipid (normally from the liver) and which has likely oxidised forming fatty acids - lowering of pH. The scales looked rather orange too which would also suggest lipid saturation but that might have been due to photographic lighting?<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class=""><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="" class="">With all good wishes, Simon.<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="" class=""><o:p class=""> </o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="" class="">Simon Moore MIScT, RSci, FLS, ACR<br class="">Conservator of Natural Sciences and Cutlery Historian,<br class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com_&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=1AeqtGsSEHoyN19MUD9LUvIHlntcw_PUKTDhYs658Ss&s=yF4AluGSnS_QHutHEYIcWHy3XdZ4wu6ULw-ATM5xJ0A&e=" style="color: purple; text-decoration: underline;" class="">www.natural-history-conservation.com</a> <o:p class=""></o:p></span></div></div><div class=""><p class="MsoNormal" style="margin: 0in 0in 12pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><span style="" class=""><br class=""><br class=""><o:p class=""></o:p></span></p></div></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class=""><blockquote style="margin-top: 5pt; margin-bottom: 5pt;" class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">On 12 Sep 2017, at 20:09, Perry Poon <<a href="mailto:Perry.Poon@ocean.org" style="color: purple; text-decoration: underline;" class="">Perry.Poon@ocean.org</a>> wrote:<o:p class=""></o:p></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Hi Simon Moore</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">The Vancouver Aquarium’s Coelacanth specimen was purchased in the fall of 1971. It was preserved in Formaldehyde at the time. </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">The Coelacanth was transferred to alcohol when it was put on display in the Gallery. There were some alcohol changes done to the display in the following years. I don’t have records of the changes</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">The last complete alcohol change (draining the old alcohol and replacing it with isopropanol) was 2000</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Since then, there has been no change in the isopropanol.</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">I’ve attached pictures of the Aquarium’s Coelacanth. You can see the deterioration around the head, fins, and the tail.</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">The pH reading for the display was done on April 12, 2017. The litmus paper indicated less than pH 5. It was pale yellow.</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Could you explain to me the hydrogen ion activity at pH 4? I’m unfamiliar with it, and it may explain the acidity.</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">There was a density meter reading made on April 12, 2017. 0.9285 g/cm<sup class="">2<span class="apple-converted-space"> </span></sup>(density meter adjusted to alcohol). The display has 51% Isopropanol (standardize to 20<span class="apple-converted-space"> </span><sup class="">o</sup>C), and 43.4% (non-standardize and not corrected to temperature)</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">You’ll see in the pictures that there are scales that have fallen from the specimen.</span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Cheers,</span><o:p class=""></o:p></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class=""><span style="font-family: Calibri, sans-serif;" class="">Perry Poon</span></b><span class="apple-converted-space"><span style="font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span></span><span style="font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><br class=""></span><span style="font-size: 10pt; font-family: Calibri, sans-serif; text-transform: uppercase;" class="">COORDINATOR, PROPS</span><o:p class=""></o:p></div></div><p class="MsoNormal" style="margin: 3pt 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><a href="mailto:Perry.Poon@ocean.org" title="Click to send email to Perry Poon" style="color: purple; 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text-decoration: underline;" class=""><span style="color: purple; text-decoration: none;" class=""><image006.png></span></a><o:p class=""></o:p></p></td></tr></tbody></table></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div></div><div class=""><div style="border-style: solid none none; border-top-width: 1pt; border-top-color: rgb(225, 225, 225); padding: 3pt 0in 0in;" class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">From:</span></b><span class="apple-converted-space"><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""> </span></span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">Simon Moore [<a href="mailto:couteaufin@btinternet.com" style="color: purple; text-decoration: underline;" class="">mailto:couteaufin@btinternet.com</a>]<span class="apple-converted-space"> </span><br class=""><b class="">Sent:</b><span class="apple-converted-space"> </span>Thursday, September 07, 2017 3:17 PM<br class=""><b class="">To:</b><span class="apple-converted-space"> </span>John E Simmons <<a href="mailto:simmons.johne@gmail.com" style="color: purple; text-decoration: underline;" class="">simmons.johne@gmail.com</a>><br class=""><b class="">Cc:</b><span class="apple-converted-space"> </span>Perry Poon <<a href="mailto:Perry.Poon@ocean.org" style="color: purple; text-decoration: underline;" class="">Perry.Poon@ocean.org</a>>;<span class="Apple-converted-space"> </span><a href="mailto:nhcoll-l@mailman.yale.edu" style="color: purple; text-decoration: underline;" class="">nhcoll-l@mailman.yale.edu</a><br class=""><b class="">Subject:</b><span class="apple-converted-space"> </span>Re: [Nhcoll-l] Coelacanth deterioration</span><o:p class=""></o:p></div></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Some history of the specimen’s initial treatment would be most helpful as John has suggested. pH 4 is the critical level for hydrogen ion activity, so you’re getting close and it obviously isn’t helping the specimen. <o:p class=""></o:p></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Beware of cosmoid scale loss as well as these can be squeezed out over time from their protein-based ‘pockets’ on the skin if these pockets start to shrink. If the specimen is preserved in alcohol then the scale loss can be repaired using Necolodine solution. Feel free to come back to me if you need any further advice or more detail and do send pics.<o:p class=""></o:p></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div><div class=""><div class=""><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">With all good wishes, Simon.<o:p class=""></o:p></div></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Simon Moore MIScT, RSci, FLS, ACR<br class="">Conservator of Natural Sciences and Cutlery Historian,<br class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com_&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=1AeqtGsSEHoyN19MUD9LUvIHlntcw_PUKTDhYs658Ss&s=yF4AluGSnS_QHutHEYIcWHy3XdZ4wu6ULw-ATM5xJ0A&e=" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">www.natural-history-conservation.com</span></a> <o:p class=""></o:p></div></div></div><div class=""><p class="MsoNormal" style="margin: 0in 0in 12pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><br class=""><br class=""><br class=""><o:p class=""></o:p></p></div></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div><div class=""><blockquote style="margin-top: 5pt; margin-bottom: 5pt;" class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">On 7 Sep 2017, at 22:11, John E Simmons <<a href="mailto:simmons.johne@gmail.com" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">simmons.johne@gmail.com</span></a>> wrote:<o:p class=""></o:p></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div><div class=""><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-family: Tahoma, sans-serif;" class="">Do you know anything about the preservation history of the specimen? For example, when was it collected; was it frozen before being preserved; was it formaldehyde-fixed or preserved directly in alcohol; has it always been in isopropyl alcohol? Some photographs of the deterioration you have noticed would be very helpful.</span><o:p class=""></o:p></div></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-family: Tahoma, sans-serif;" class=""> </span><o:p class=""></o:p></div></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-family: Tahoma, sans-serif;" class="">--John</span><o:p class=""></o:p></div></div></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><br clear="all" class=""><o:p class=""></o:p></div></div><div class=""><div class=""><div class=""><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">John E. Simmons<br class="">Museologica<br class="">128 E. Burnside Street<br class="">Bellefonte, Pennsylvania 16823-2010<br class=""><a href="mailto:simmons.johne@gmail.com" target="_blank" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">simmons.johne@gmail.com</span></a><br class="">303-681-5708<br class=""><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.museologica.com&d=DwMFaQ&c=cjytLXgP8ixuoHflwc-poQ&r=LpYc_Z_iN1KRw0hheb3x6-8MJUMu482qfHowpGYJqwc&m=ojZwthtm_RvMiyLAKyHGiNNou78SaQuloQ8tfqMCRR4&s=znaiaoXBwnNGn1ee56RHUyjy0SUpa9aXPKTUGddiSAw&e=" target="_blank" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">www.museologica.com</span></a><br class="">and<br class="">Adjunct Curator of Collections<br class="">Earth and Mineral Science Museum & Art Gallery<br class="">Penn State University<br class="">University Park, Pennsylvania<br class="">and<br class="">Instructor, Museum Studies<br class="">School of Library and Information Science<br class="">Kent State University<o:p class=""></o:p></div></div></div></div></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">On Thu, Sep 7, 2017 at 4:26 PM, Perry Poon <<a href="mailto:Perry.Poon@ocean.org" target="_blank" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">Perry.Poon@ocean.org</span></a>> wrote:<o:p class=""></o:p></div></div><blockquote style="border-style: none none none solid; border-left-width: 1pt; border-left-color: rgb(204, 204, 204); padding: 0in 0in 0in 6pt; margin: 5pt 0in 5pt 4.8pt;" class=""><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">I am an employee of the Vancouver Aquarium in British Columbia. I am looking for advice in the deteriorating nature of the Aquarium’s Coelacanth. It is currently in 50% isopropanol, and the pH is less than 5.<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Deterioration is around the head, fin membranes , and towards the tail.<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Looking for expert advice, and I will send pictures to interested parties<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""> <o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Cheers,<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class="">Perry Poon</b><span class="apple-converted-space"> </span><br class=""><span style="font-size: 10pt; text-transform: uppercase;" class="">COORDINATOR, PROPS</span><o:p class=""></o:p></div></div><p class="MsoNormal" style="margin: 3pt 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;"><a href="mailto:Perry.Poon@ocean.org" target="_blank" title="Click to send email to Perry Poon" style="color: purple; text-decoration: underline;" class=""><span style="color: rgb(0, 94, 184);" class="">Perry.Poon@ocean.org</span></a><span class="apple-converted-space"> </span><br class="">Vancouver Aquarium<o:p class=""></o:p></p><p class="MsoNormal" style="margin: 3pt 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;">British Columbia<o:p class=""></o:p></p><p class="MsoNormal" style="margin: 3pt 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;">Canada<o:p class=""></o:p></p><p class="MsoNormal" style="margin: 3pt 0in 0.0001pt; 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