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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Hi Tonya,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">I’d simply move to 70% ethanol to balance preservation versus dehydration effects. I don’t see any value taking the specimens back
to aqueous formaldehyde (‘formalin’) once they have been preserved in this way. Ethanol can be treated as a ‘pseudofixative’ due to the way it dehydrates tissues and subsequently alter protein arrangements.
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">All the best<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Jules<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Prif Gadwraethydd, Gwyddorau Naturiol
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Principal Conservator Natural Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><a href="mailto:julian.carter@amgueddfacymru.ac.uk"><span style="color:#0563C1">julian.carter@amgueddfacymru.ac.uk</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><a href="mailto:julian.carter@museumwales.ac.uk"><span style="color:#0563C1">julian.carter@museumwales.ac.uk</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">https://www.researchgate.net/profile/Julian_Carter<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">029 20573230<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">07870448074</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif"> Nhcoll-l [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Simon Moore<br>
<b>Sent:</b> 13 December 2018 08:57<br>
<b>To:</b> Tonya.Haff@csiro.au<br>
<b>Cc:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] Small herps in ETOH<o:p></o:p></span></p>
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<p class="MsoNormal">Hi Tonya,<o:p></o:p></p>
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<p class="MsoNormal">You should be OK to inject the specimens with formalin. Might need to take them down a short hydration ladder to water, then fix overnight (and inject) before taking them back up again into preserving EtOH. I always maintain a few small
tanks for 'intermediate alcohols' for this purpose as it helps to reduce the effect of syneresis (osmotic shrivelling).<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">With all good wishes, Simon.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">Simon Moore MIScT, RSci, FLS, ACR<br>
Conservator of Natural Sciences and Cutlery Historian, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black"><a href="http://www.natural-history-conservation.com">www.natural-history-conservation.com</a> <o:p></o:p></span></p>
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<p class="MsoNormal">On 13 Dec 2018, at 03:56, <<a href="mailto:Tonya.Haff@csiro.au">Tonya.Haff@csiro.au</a>> <<a href="mailto:Tonya.Haff@csiro.au">Tonya.Haff@csiro.au</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Hi all,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">We were recently donated a few salvaged small snakes and lizards (largest is probably ~30 g) that have been chucked directly in alcohol (the lizards 95%, the snake 70%). Normally,
I would prep herps in formalin first, and then step into ETOH. Do any of you have recommendations for how best to deal with these specimens to ensure they preserve well long term? Is it too late, or not smart, to fix them in formalin now? The very small lizards
(skinks) seem fine, and have been in their jars for a few years with almost no slippage. The snake has only recently been placed into 70% alcohol.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Thanks for your help!<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Cheers,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Tonya<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">---------------------------------------------------------<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Dr Tonya Haff<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Collections Manager<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Australian National Wildlife Collection<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">National Research Collections Australia, CSIRO<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Canberra, Australia<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Phone:<span class="apple-converted-space"> </span><a href="tel:(+61)%2002%2062421566"><span style="color:#954F72">(+61) 02 62421566</span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
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