<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:Wingdings;
panose-1:5 0 0 0 0 0 0 0 0 0;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#0563C1;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:#954F72;
text-decoration:underline;}
p.msonormal0, li.msonormal0, div.msonormal0
{mso-style-name:msonormal;
mso-margin-top-alt:auto;
margin-right:0in;
mso-margin-bottom-alt:auto;
margin-left:0in;
font-size:12.0pt;
font-family:"Times New Roman",serif;}
span.EmailStyle18
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle19
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:#1F497D;}
span.EmailStyle20
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle21
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:#1F497D;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="#0563C1" vlink="#954F72">
<div class="WordSection1">
<p class="MsoNormal"><span style="color:#1F497D">Paul<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">We very rarely have whole specimens stored in ethanol for DNA extraction. In most cases, tissue samples are removed from vouchers and stored in our cryogenic facility and the vouchers fixed in formalin and then
preserved in ethanol. We don’t use a fully non-permeable solution but instead use polyester fiber batting. Again, I doubt that there would be much if any cross-contamination as the fluid is not moving or mixing very much within the jar and, as mentioned
before, I doubt that there would be much in the liquid anyway but I may be wrong.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Andy<o:p></o:p></span></p>
<div>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU" style="color:#1F497D"> A : A : A :<br>
}<(((_�>.,.,.,.}<(((_�>.,.,.,.}<)))_�><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU" style="color:#1F497D">Dyche Hall<br>
<a href="x-apple-data-detectors://9">1345 Jayhawk Boulevard</a><br>
<a href="x-apple-data-detectors://9">Lawrence, KS, 66045-7561</a><br>
<a href="x-apple-data-detectors://9">USA</a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank">(785) 864-3863</a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank">(785) 864-5335</a> <br>
Email: <a href="mailto:abentley@ku.edu" target="_blank">abentley@ku.edu</a> </span><span style="color:#1F497D"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU" style="color:#1F497D"><a href="http://ichthyology.biodiversity.ku.edu/" target="_blank">http://ichthyology.biodiversity.ku.edu</a></span><span style="color:#1F497D"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="color:#1F497D"> A : A : A :<br>
}<(((_�>.,.,.,.}<(((_�>.,.,.,.}<)))_�><br>
V V V<o:p></o:p></span></p>
</div>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b>From:</b> Callomon,Paul <prc44@drexel.edu> <br>
<b>Sent:</b> Friday, February 22, 2019 9:03 AM<br>
<b>To:</b> Bentley, Andrew Charles <abentley@ku.edu>; Thomas Labedz <tlabedz1@unl.edu>; NHCOLL-L@mailman.yale.edu<br>
<b>Subject:</b> RE: eDNA voucher question<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Hi Andy,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">What kind of stopper do you use in vials that are submerged in fluid? Presumably they must be impermeable, to prevent cross-contamination, but does e. g. polypropylene last well under full immersion? I know that phenolic does not – it will
leach into alcohol after only a few years. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">PC<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="background:white"><b><span style="font-size:10.0pt;color:black">Paul Callomon</span></b><span style="font-size:10.0pt;color:black"><br>
<i>Collection Manager, Malacology and General Invertebrates</i></span><span style="color:black"><o:p></o:p></span></p>
<div class="MsoNormal" align="center" style="text-align:center;background:white">
<span style="font-size:10.0pt;color:black">
<hr size="2" width="100%" align="center">
</span></div>
<p class="MsoNormal" style="background:white"><b><span style="font-size:10.0pt;color:black">Academy of Natural Sciences of Drexel University, Philadelphia<o:p></o:p></span></b></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;color:black">1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA<br>
<i><a href="mailto:callomon@ansp.org" target="_blank"><span style="color:blue">callomon@ansp.org</span></a> Tel 215-405-5096 - Fax 215-299-1170</i></span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b>From:</b> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a>>
<b>On Behalf Of </b>Bentley, Andrew Charles<br>
<b>Sent:</b> Friday, February 22, 2019 10:01 AM<br>
<b>To:</b> Thomas Labedz <<a href="mailto:tlabedz1@unl.edu">tlabedz1@unl.edu</a>>;
<a href="mailto:NHCOLL-L@mailman.yale.edu">NHCOLL-L@mailman.yale.edu</a><br>
<b>Subject:</b> Re: [Nhcoll-l] eDNA voucher question<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Tom</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">We have used vials within jars to keep tissue vouchers separate and prevent cross-contamination. You can vary the vials size based on the size of the fish and as such it shouldn’t take up too much more space
– even though it will take up a lot more of your time </span><span style="font-family:Wingdings;color:#1F497D">J</span><span style="color:#1F497D">. Having said that I wonder how much cross-contamination you would get after the fish have been preserved?
Not sure that there would be much floating around in the liquid after preservation that wouldn’t be drowned out by sending a tissue sample from the original fish for analysis.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">My two cents…</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Andy</span><o:p></o:p></p>
<div>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU" style="color:#1F497D"> A : A : A :<br>
}<(((_�>.,.,.,.}<(((_�>.,.,.,.}<)))_�><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute</span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU" style="color:#1F497D">Dyche Hall<br>
<a href="x-apple-data-detectors://9">1345 Jayhawk Boulevard</a><br>
<a href="x-apple-data-detectors://9">Lawrence, KS, 66045-7561</a><br>
<a href="x-apple-data-detectors://9">USA</a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank">(785) 864-3863</a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank">(785) 864-5335</a> <br>
Email: <a href="mailto:abentley@ku.edu" target="_blank">abentley@ku.edu</a> </span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU" style="color:#1F497D"><a href="https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fichthyology.biodiversity.ku.edu%2F&data=02%7C01%7Cabentley%40ku.edu%7C6a0ffbd45c9b495a179808d698d6e3f8%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C636864446047168646&sdata=wd1%2F1DADxuJTdA1wkcZYYAEcvDX2ezGmVjvNWqFv0T4%3D&reserved=0" target="_blank">http://ichthyology.biodiversity.ku.edu</a></span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="color:#1F497D"> A : A : A :<br>
}<(((_�>.,.,.,.}<(((_�>.,.,.,.}<)))_�><br>
V V V</span><o:p></o:p></p>
</div>
<p class="MsoNormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b>From:</b> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a>>
<b>On Behalf Of </b>Thomas Labedz<br>
<b>Sent:</b> Thursday, February 21, 2019 3:42 PM<br>
<b>To:</b> <a href="mailto:NHCOLL-L@mailman.yale.edu">NHCOLL-L@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] eDNA voucher question<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">Greetings from snowy central North America!<o:p></o:p></p>
<p class="MsoNormal">Has anyone developed policy or protocol for the receipt, treatment, and maintenance of eDNA fish voucher specimens? Would you share? The individual fish are small and the researcher recommends keeping each individual in a separate container
(how they were delivered) to prohibit cross contamination of DNA via the ethanol solution. That is understandable but will utilize lots of space and containers.<o:p></o:p></p>
<p class="MsoNormal">Thomas<o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif">Thomas E. Labedz, Collections Manager</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif">Division of Zoology and Division of Botany</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif">University of Nebraska State Museum</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif">Lincoln, Nebraska, U.S.A.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif"><a href="mailto:tlabedz1@unl.edu">tlabedz1@unl.edu</a>
</span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
</div>
</body>
</html>