<div dir="ltr"><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000">Tom,</div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000">I am not sure I understand the question. Do you mean the fish are vouchers from which DNA has been extracted and you want to keep the specimen separate to link it to the DNA sample, or do you mean fish from which DNA will be extracted in the future? <br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000">You will get contamination of the alcohol around the specimen from DNA from other specimens if you house them all together because protein is extracted over time by the alcohol (for example, see von Endt 1994, "Spirit collections: a preliminary analysis of some organic materials found in the storage fluid of mammals," Collection Forum 10(1):10-19). Whether that contamination would affect the DNA in the fish specimen itself has not been looked at, to my knowledge, but is an interesting question.</div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000">If you are storing these as whole-specimen vouchers from which DNA has already been sampled, I would go with Andy's suggestion of vials with polyester fiber plugs. If you are storing these for future DNA extraction, then they should be in 95.6% ETOH if they must be kept in alcohol.</div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000">Please let me know if I have misunderstood your question.</div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000">--John</div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:#000000"><br clear="all"></div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><font size="2"><span style="font-family:tahoma,sans-serif">John E. Simmons<br>Writer and Museum Consultant</span></font></div><div dir="ltr"><font size="2"><span style="font-family:tahoma,sans-serif">Museologica<br><i>and</i><br>Associate Curator of Collections<br>Earth and Mineral Science Museum & Art Gallery<br>Penn State University<br><i>and</i><br>Investigador Asociado, Departamento de Ornitologia<br>Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, Lima</span></font><br></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div><br></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 21, 2019 at 4:41 PM Thomas Labedz <<a href="mailto:tlabedz1@unl.edu">tlabedz1@unl.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
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<p class="MsoNormal">Greetings from snowy central North America!<u></u><u></u></p>
<p class="MsoNormal">Has anyone developed policy or protocol for the receipt, treatment, and maintenance of eDNA fish voucher specimens? Would you share? The individual fish are small and the researcher recommends keeping each individual in a separate container
(how they were delivered) to prohibit cross contamination of DNA via the ethanol solution. That is understandable but will utilize lots of space and containers.<u></u><u></u></p>
<p class="MsoNormal">Thomas<u></u><u></u></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:"Arial",sans-serif">Thomas E. Labedz, Collections Manager<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:"Arial",sans-serif">Division of Zoology and Division of Botany<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:"Arial",sans-serif">University of Nebraska State Museum<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:"Arial",sans-serif">Lincoln, Nebraska, U.S.A.<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:"Arial",sans-serif"><a href="mailto:tlabedz1@unl.edu" target="_blank">tlabedz1@unl.edu</a>
<u></u><u></u></span></p>
<p class="MsoNormal"><u></u> <u></u></p>
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