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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">I agree with Dean. Have used absinthe for mussels as well – it was expensive, but I was in a pinch, and it was the highest proof around. Transferred to 95%
EtOH when back in the lab after a few days and DNA extraction, PCR and sequencing worked fine. Absinthe arguably should be worse for the DNA than Everclear, but it worked.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">PW<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> Nhcoll-l [mailto:nhcoll-l-bounces@mailman.yale.edu]
<b>On Behalf Of </b>Dean Pentcheff<br>
<b>Sent:</b> Friday, April 19, 2019 11:50 AM<br>
<b>To:</b> Nick Cairns <nacairns@gmail.com>; nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] Use of denatured ethanol for short term storage of molecular samples<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We have had excellent results on Sanger sequencing (haven't tried genomic) using Everclear (high-proof drinking alcohol). Specimens were preserved in Everclear, then transferred to 95% ethanol a few days later. These were small freshwater
crustaceans (aquatic isopods).<o:p></o:p></p>
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<p class="MsoNormal">I'd be more inclined to go towards drinkable alcohol rather than denatured alcohol — if it's safe for human consumption, there probably isn't too much bioactive chemistry going on (other than the ethanol itself). <o:p></o:p></p>
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<p class="MsoNormal">-Dean<br>
-- <br>
Dean Pentcheff<br>
<a href="mailto:pentcheff@gmail.com" target="_blank">pentcheff@gmail.com</a><o:p></o:p></p>
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<p class="MsoNormal"><a href="mailto:dpentche@nhm.org" target="_blank">dpentche@nhm.org</a><br>
<a href="https://research.nhm.org/disco" target="_blank">https://research.nhm.org/disco</a><o:p></o:p></p>
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<p class="MsoNormal"><img border="0" id="_x0000_i1025" src="http://research.nhm.org/images/DISCO_lockup_4color-300.png"><o:p></o:p></p>
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<p class="MsoNormal">On Thu, Apr 18, 2019 at 2:04 PM Nick Cairns <<a href="mailto:nacairns@gmail.com" target="_blank">nacairns@gmail.com</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">Hello everyone,<o:p></o:p></p>
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<p class="MsoNormal">I'm seeking guidance on reagents. I'm trying to collect chorus frogs from across western Canada (whole and toe clips). These samples will be likely be extracted using phenol/chloroform then ethanol (EtOH) precipitation to tidy them up.
Downstream they'll be used for mtDNA (Sanger) and genomic (ddRAD) protocols. The issue is, I am currently in rural Saskatchewan and only have denatured EtOH (Fisherbrand Histoprep 95%) available to me. I understand that the additives in some denatured EtOH
can cause issues downstream but has anyone ever used it for short term storage then replaced it later with
<span class="gmail-m6214145799784408086gmail-m-3394905990569747990gmail-st">anhydrous</span> to remove the additives? Can these additives be reduced from the tissues after the fact?
<o:p></o:p></p>
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<p class="MsoNormal">Any insights would be most welcome. <o:p></o:p></p>
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<p class="MsoNormal">Thank you,<o:p></o:p></p>
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<p class="MsoNormal">Nick<o:p></o:p></p>
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