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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Thanks Paul,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Useful examples. I know that some of my comments came from zooplankton experts.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">It is possible that observations for specific species of jellies and other zooplankters may have been apply to the broader group as a precautionary approach.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">I don’t think it was an urban legend… ;0)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Jean-Marc<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> Callomon,Paul [mailto:prc44@drexel.edu]
<br>
<b>Sent:</b> July 9, 2020 2:26 PM<br>
<b>To:</b> Jean-Marc Gagnon <JMGAGNON@nature.ca>; Bentley, Andrew Charles <abentley@ku.edu>; Curtis Schmidt <cjschmidt@fhsu.edu>; nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] preserving freshwater jellyfish (Hydrozoans)<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">On display in the department we have a jar of six Cannonball Jellyfish (Stomolophus meleagris) in ethanol that are in fine condition, floating around like a lava lamp 50 years after
collection. We also have a pair of Box Jellyfish that I moved from an unknown fluid to 80% ethanol about 15 years ago, and they too remain positively buoyant and in good, non-opaque condition (they might have been formalin-fixed). Finally I have a Pyrosome
(tunicate) in 80% ethanol that is perfect after 18 years. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">For many years I had a fine Velella that floated perfectly in 70% ethanol, but a few years ago its "sail" began to deflate and it eventually sank to the bottom of the jar. It seems
OK otherwise, though.<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">Paul Callomon</span></b><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><br>
<i>Collection Manager, Malacology and General Invertebrates</i><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:black">Academy of Natural Sciences of Drexel University, Philadelphia</span></b><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><br>
<i><a href="mailto:callomon@ansp.org">callomon@ansp.org</a> Tel 215-405-5096 - Fax 215-299-1170</i><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a>>
on behalf of Jean-Marc Gagnon <<a href="mailto:JMGAGNON@nature.ca">JMGAGNON@nature.ca</a>><br>
<b>Sent:</b> Thursday, July 9, 2020 1:16 PM<br>
<b>To:</b> Bentley, Andrew Charles <<a href="mailto:abentley@ku.edu">abentley@ku.edu</a>>; Curtis Schmidt <<a href="mailto:cjschmidt@fhsu.edu">cjschmidt@fhsu.edu</a>>;
<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a> <<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a>><br>
<b>Subject:</b> Re: [Nhcoll-l] preserving freshwater jellyfish (Hydrozoans)</span>
<o:p></o:p></p>
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<p><b><span style="font-family:"Arial",sans-serif;font-variant:small-caps">External.<o:p></o:p></span></b></p>
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<p class="xmsonormal"><span style="color:#1F497D">Curtis,</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D">Over my 40 years of work with invertebrates, I have to admit that I never had to fix and/or preserved jellies; my specialty being marine benthos. But I have been told a number of things about the preservation
of plankton. </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D">One is that some jellies do not like ethanol at all, even after fixation with formaldehyde. I believe that is the case for our small FW medusa
<i>Craspedacusta sowerbii</i>. </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D">The other issue, which is not necessarily one for jellies, is that specimens can become opaque once transferred in ethanol, hence obscuring internal structures that may normally be used for identification or
determining the life stage.</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D">In both cases, best to leave them in formaldehyde if possible.
</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D">I certainly would like to hear from others on the subject.</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D">Jean-Marc</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><b><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Jean-Marc Gagnon</span></b><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">, Ph.D.</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Curator, Invertebrate Collections / Chief Scientist</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Conservateur, Collection des invertébrés / Expert scientifique en chef</span><o:p></o:p></p>
<p class="xmsonormal"><b><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Canadian Museum of Nature / Musée canadien de la nature</span></b><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">613 364 4066</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">613 851-7556 cell</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">613 364 4027 Fax</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D"><a href="mailto:jmgagnon@nature.ca"><span style="color:#0563C1">jmgagnon@nature.ca</span></a>
</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><u><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Adresse postale / Postal Address:</span></u><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Canadian Museum of Nature / Musée canadien de la nature</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">P.O. Box 3443, Sta.
</span><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">D / Casier Postal 3443, Succ. D</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Ottawa, ON K1P 6P4 / Ottawa, ON K1P 6P4</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Canada / Canada</span><o:p></o:p></p>
<p class="xmsonormal"><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><u><span lang="FR-CA" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">Adresse de livraison / Courier Address :
</span></u><o:p></o:p></p>
<p class="xmsonormal"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#1F497D">1740 Pink Road, Gatineau, QC, J9J 3N7</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
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<p class="xmsonormal"><b>From:</b> Nhcoll-l [<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">mailto:nhcoll-l-bounces@mailman.yale.edu</a>]
<b>On Behalf Of </b>Bentley, Andrew Charles<br>
<b>Sent:</b> July 8, 2020 11:23 PM<br>
<b>To:</b> Curtis Schmidt <<a href="mailto:cjschmidt@fhsu.edu">cjschmidt@fhsu.edu</a>>;
<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> Re: [Nhcoll-l] preserving freshwater jellyfish (Hydrozoans)<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
<p class="xmsonormal"><a name="x__GoBack"></a><span class="xemailstyle18"><span style="font-family:"Calibri",sans-serif">Curtis</span></span><o:p></o:p></p>
<p class="xmsonormal"><span class="xemailstyle18"><span style="font-family:"Calibri",sans-serif"> </span></span><o:p></o:p></p>
<p class="xmsonormal"><span class="xemailstyle18"><span style="font-family:"Calibri",sans-serif">You may want to check this out:</span></span><o:p></o:p></p>
<p class="xmsonormal"><span class="xemailstyle18"><span style="font-family:"Calibri",sans-serif"> </span></span><o:p></o:p></p>
<p class="xmsonormal"><a href="https://nam01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.austincc.edu%2Fsziser%2FBiol%25201413%2Fanimal%2520collections%2FSoft%2520Bodied%2520Invertebrates.pdf&data=02%7C01%7Cprc44%40drexel.edu%7C3be8f27744494ac597d608d8242be266%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637299118191596899&sdata=Z70QObwown1wXduM0cFZEldulwV745OE3feZOQrdSUs%3D&reserved=0">https://www.austincc.edu/sziser/Biol%201413/animal%20collections/Soft%20Bodied%20Invertebrates.pdf</a><o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
<p class="xmsonormal">Doesn’t address hydrozoans specifically but other soft-bodied organisms.<o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
<p class="xmsonormal">Then there is this: <a href="https://nam01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.si.edu%2Fes%2Fobject%2Fyt_ry67AXwxM9o&data=02%7C01%7Cprc44%40drexel.edu%7C3be8f27744494ac597d608d8242be266%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637299118191606894&sdata=Pu6j38Ks2fLs8Bxo2F8tLO4RLsWFUIMF930N8POYpag%3D&reserved=0">
https://www.si.edu/es/object/yt_ry67AXwxM9o</a><o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
<p class="xmsonormal">Andy<o:p></o:p></p>
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<p class="xmsonormal"><span style="color:black"> A : A : A :</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°></span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> V V V</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Andy Bentley</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Ichthyology Collection Manager</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">University of Kansas</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Biodiversity Institute</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Dyche Hall</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">1345 Jayhawk Boulevard</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Lawrence, KS, 66045-7561</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">USA</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Tel: (785) 864-3863</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Fax: (785) 864-5335 </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Email: </span><a href="mailto:abentley@ku.edu">abentley@ku.edu</a><span style="color:black"> </span><o:p></o:p></p>
<p class="xmsonormal"><a href="https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fichthyology.biodiversity.ku.edu%2F&data=02%7C01%7Cprc44%40drexel.edu%7C3be8f27744494ac597d608d8242be266%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637299118191606894&sdata=RbKl62jwSzbqOD0Smni0BtzWDKp%2BGew7P18mwCMFxXo%3D&reserved=0">http://ichthyology.biodiversity.ku.edu</a><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> A : A : A :</span><o:p></o:p></p>
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<p class="xmsonormal"><b><span style="color:black">From: </span></b><span style="color:black">Nhcoll-l <</span><a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a><span style="color:black">> on behalf of "</span><a href="mailto:cjschmidt@fhsu.edu">cjschmidt@fhsu.edu</a><span style="color:black">"
<</span><a href="mailto:cjschmidt@fhsu.edu">cjschmidt@fhsu.edu</a><span style="color:black">><br>
<b>Date: </b>Wednesday, July 8, 2020 at 9:37 PM<br>
<b>To: </b>"</span><a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><span style="color:black">" <</span><a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><span style="color:black">><br>
<b>Subject: </b>[Nhcoll-l] preserving freshwater jellyfish (Hydrozoans)</span><o:p></o:p></p>
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<p class="xmsonormal"><span style="color:black">All,</span><o:p></o:p></p>
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<p class="xmsonormal"><span style="color:black">I am wanting to know the best way to preserve and store freshwater Hydrozoans. Formalin? Ethanol? Hand Sanitizer? Any and all information would be greatly appreciated. Thank you!</span><o:p></o:p></p>
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<p class="xmsonormal"><span style="color:black">Curtis</span><o:p></o:p></p>
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<p style="background:white"><span style="color:black">Curtis J. Schmidt</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> Zoological Collections Manager</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> Sternberg Museum of Natural History</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> </span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> Instructor</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> Department of Biological Sciences</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> Fort Hays State University</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> </span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> 3000 Sternberg Drive</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> Hays, KS 67601</span><o:p></o:p></p>
<p style="background:white"><span style="color:black"> 785-650-2447 (cell)</span><o:p></o:p></p>
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