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<div class="moz-cite-prefix">Hi Tonya,</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">there are surely a couple of things
here: <br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">1. Depending which jars and closures
you use in your collection, you will have different expansion
coefficients. Containers for cryo-storage usually consider this
(and some even will get properly tight when frozen and the lid
shrinks under cold temperatures to give a proper fit), but jars we
normally use in our collections are not designed for such cold
temperatures. Freezing them, could cause considerable damage to
jars and/or lids and may lead to high replacement costs in the
long run.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">2. Lipids, oils, fats and other
dissolved components might coagulate. For example in insect
collections, waxy deposits are commonly observed when specimens
are frozen (e.g. -25°C). These deposits will not disappear when
the specimens are thawed again but remain, covering all the
filigree appendices, antennae, etc. In larger specimens, e.g.
fish, it could lead to e.g to cholesterol deposits on specimens.
So again, you surely would introduce more damage when freezing the
jars.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">3. Changing the temperature regime also
influences the chemical equilibrium that formed inside specimen
jars. Besides lipids and oils, lower temperatures usually decrease
the capacity of the preservation fluid to keep organic compounds
in solution. This often triggers crystallisation processes, e.g.
residual dissolved formaldehyde may form paraformaldehyde needles.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Carrying potential pests into your new
storage with the jars would only be possible if you would move a
lot of organic material into your new storage area. Some organic
material, such as historic big bladder seals, are also very
vulnerable to low temperatures (shrinkage). So if there are no
organic deposits on your jars, there wouldn't be much nutrients
that could be carried. Vice versa, if there would be dust on the
jar (as we have currently because of major renovation works going
on in our collection), cleaning jars with a damp cloth should do
the job. You should be careful with external labels in this case,
but usually any 'dirt' would be on the top. In case of stoppered
jars or jars with historic seals, you probably would need to be
careful with damp cleaning. <br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Hope this helps,</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">and good progress with your moving!</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">With best wishes <br>
</div>
<div class="moz-cite-prefix">Dirk<br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Am 06.08.2020 um 08:25 schrieb Haff,
Tonya (NCMI, Crace):<br>
</div>
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<div class="WordSection1">
<p class="MsoNormal">Hi all,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have a question for you about freezing
whole jars of specimens – what do you think the effect would
be, and would you recommend it? My gut says NOOOOOOOO this is
a terrible idea, but it has been suggested as a shortcut to
kill potential pests before we move the collection into a new
building. I personally think that the main thing we don’t want
to move into a new EtOH vault is mould, whose spores won’t be
killed by freezing anyway. But, I am open to ideas and I
wanted to check with other people before loudly stamping down
my foot…<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks!<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Tonya<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">---------------------------------------------------------<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">Dr
Tonya Haff<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">Collections
Manager<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">Australian
National Wildlife Collection<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">National
Research Collections Australia, CSIRO<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">Canberra,
Australia
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">Phone:
(+61) 02 6242 1566 (office)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">(+61)
0419 569 109 (mobile)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">I
am in Thursdays and Fridays
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU">Please
call or text my mobile for a fast reponse Monday – Wednesday<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<br>
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<div class="moz-signature">-- <br>
<p><img src="cid:part1.D3077D15.9B6D5DFE@snsb.de" alt=""
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<p><br>
Dirk Neumann<br>
<br>
Tel: 089 / 8107-111<br>
Fax: 089 / 8107-300<br>
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<br>
Postanschrift:<br>
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Münchhausenstr. 21<br>
81247 München<br>
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Besuchen Sie unsere Sammlung:<br>
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---------<br>
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Dirk Neumann<br>
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Tel: +49-89-8107-111<br>
Fax: +49-89-8107-300<br>
neumann(a)snsb.de<br>
<br>
postal address:<br>
<br>
Bavarian Natural History Collections<br>
The Bavarian State Collection of Zoology<br>
Dirk Neumann, Section Ichthyology / DNA-Storage<br>
Muenchhausenstr. 21<br>
81247 Munich (Germany)<br>
<br>
Visit our section at:<br>
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