<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Consolas;
panose-1:2 11 6 9 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
pre
{mso-style-priority:99;
mso-style-link:"HTML Preformatted Char";
margin:0in;
margin-bottom:.0001pt;
font-size:10.0pt;
font-family:"Courier New";}
span.HTMLPreformattedChar
{mso-style-name:"HTML Preformatted Char";
mso-style-priority:99;
mso-style-link:"HTML Preformatted";
font-family:Consolas;}
span.EmailStyle21
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="blue" vlink="purple">
<div class="WordSection1">
<p class="MsoNormal"><a name="_GoBack"></a>John<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have been using these Nalgene tubes in our collection and for collecting in all environments for over 15 years and have had no issues with storage in 95% ethanol, RNALater or liquid nitrogen environments:
<a href="https://www.fishersci.com/shop/products/nalgene-general-long-term-storage-cryogenic-tubes/033377d">
https://www.fishersci.com/shop/products/nalgene-general-long-term-storage-cryogenic-tubes/033377d</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I agree with Dirk that whatever make you go with, externally threaded tubes with no O-ring gasket are the way to go.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Andy<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div>
<p class="MsoNormal"><span style="color:black"> A : A : A :<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> V V V<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Andy Bentley<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Ichthyology Collection Manager<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">University of Kansas<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Dyche Hall<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">1345 Jayhawk Boulevard<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Lawrence, KS, 66045-7561<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">USA<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Tel: (785) 864-3863<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Fax: (785) 864-5335 <o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Email: <a href="mailto:abentley@ku.edu">abentley@ku.edu</a> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"><a href="http://ichthyology.biodiversity.ku.edu/">http://ichthyology.biodiversity.ku.edu</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> A : A : A :<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> V V V<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<div style="border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> on behalf of Dirk Neumann <neumann@snsb.de><br>
<b>Reply-To: </b>"neumann@snsb.de" <neumann@snsb.de><br>
<b>Date: </b>Wednesday, August 19, 2020 at 4:05 PM<br>
<b>To: </b>"nhcoll-l@mailman.yale.edu" <nhcoll-l@mailman.yale.edu><br>
<b>Subject: </b>Re: [Nhcoll-l] comparative quality cryogenic vials<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Dear John,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">I would prefer NUNC Store-it tubes (article 264300; <a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.thermofisher.com%2Forder%2Fcatalog%2Fproduct%2F264262%23%2F264262&data=02%7C01%7Cabentley%40ku.edu%7Cdf385ddaaca24707b7f808d844839aa4%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637334679345844493&sdata=2fl7vyz6UNbMvx1fHGiytMNCT9cN3ggCZ%2BkbqTVaI1I%3D&reserved=0">
https://www.thermofisher.com/order/catalog/product/264262#/264262</a>)<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">We tested them against Sarsted and other brands in a warm water bath > 50°C and they were among the tightest ones; also, they DON'T have an silicone O-ring (which will fail after some time); we had to replace poor quality tubes twice so
far and besides necessary replacement costs and time needed to transfer tissues the huge evaporation loss (> 50% in some tubes) surely affected the integrity of the tissues / DNA.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">The NUNC-tubes have a flange and really need to be screwed down tight (which is a bit annoying), but we have them on storage since 2004, use them in the tropic in the field and their performance is excellent. Bit more expensive, but this
slightly higher investment pays off in the long run.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Hope this helps,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">with best wishes<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Dirk<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Am 17.08.2020 um 23:42 schrieb John Edwin Lattke Bravo:<o:p></o:p></p>
</div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<div>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Hello,<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Does anybody have any thoughts or experience to share regarding the comparative merits of 2ml cryogenic vials by Corning, Sarstedt and Granier for long term storage of insects in ethanol? Is the
quality amongst the three similar? Positive experiences? Negative one? Just needing some user experience to help decide what might deliever the best bang for the buck (or real, in my case).<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black">Obrigado, <o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black">John Lattke<o:p></o:p></span></p>
</div>
<p class="MsoNormal"><br>
<br>
<o:p></o:p></p>
<pre>_______________________________________________<o:p></o:p></pre>
<pre>Nhcoll-l mailing list<o:p></o:p></pre>
<pre><a href="mailto:Nhcoll-l@mailman.yale.edu">Nhcoll-l@mailman.yale.edu</a><o:p></o:p></pre>
<pre><a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fmailman.yale.edu%2Fmailman%2Flistinfo%2Fnhcoll-l&data=02%7C01%7Cabentley%40ku.edu%7Cdf385ddaaca24707b7f808d844839aa4%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637334679345844493&sdata=TiFYyC1XKSVNZ5mAp%2BqlOtDKIDVRHgjAEclzkVCdRl4%3D&reserved=0">https://mailman.yale.edu/mailman/listinfo/nhcoll-l</a><o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>_______________________________________________<o:p></o:p></pre>
<pre>NHCOLL-L is brought to you by the Society for the Preservation of<o:p></o:p></pre>
<pre>Natural History Collections (SPNHC), an international society whose<o:p></o:p></pre>
<pre>mission is to improve the preservation, conservation and management of<o:p></o:p></pre>
<pre>natural history collections to ensure their continuing value to<o:p></o:p></pre>
<pre>society. See <a href="https://nam10.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.spnhc.org%2F&data=02%7C01%7Cabentley%40ku.edu%7Cdf385ddaaca24707b7f808d844839aa4%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637334679345854491&sdata=qVOmwDsg6lDAX7banvgNPe0yPJv1y%2FfnbyOAMOqtIBw%3D&reserved=0">http://www.spnhc.org</a> for membership information.<o:p></o:p></pre>
<pre>Advertising on NH-COLL-L is inappropriate.<o:p></o:p></pre>
</blockquote>
<p><o:p> </o:p></p>
<div>
<p class="MsoNormal">-- <o:p></o:p></p>
<p><img border="0" width="152" height="59" style="width:1.5833in;height:.6145in" id="_x0000_i1025" src="cid:image001.png@01D67644.845D2980"><o:p></o:p></p>
<p style="margin-bottom:12.0pt"><br>
Dirk Neumann<br>
<br>
Tel: 089 / 8107-111<br>
Fax: 089 / 8107-300<br>
neumann(a)snsb.de<br>
<br>
Postanschrift:<br>
<br>
Staatliche Naturwissenschaftliche Sammlungen Bayerns<br>
Zoologische Staatssammlung München<br>
Dirk Neumann, Sektion Ichthyologie / DNA-Storage<br>
Münchhausenstr. 21<br>
81247 München<br>
<br>
Besuchen Sie unsere Sammlung:<br>
<a href="https://nam10.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.zsm.mwn.de%2Fsektion%2Fichthyologie-home%2F&data=02%7C01%7Cabentley%40ku.edu%7Cdf385ddaaca24707b7f808d844839aa4%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637334679345854491&sdata=81X4GwJlwQqBKS6t6LUsr5ieQ8LJze3TClDRVBHuXww%3D&reserved=0">http://www.zsm.mwn.de/sektion/ichthyologie-home/</a><br>
<br>
---------<br>
<br>
Dirk Neumann<br>
<br>
Tel: +49-89-8107-111<br>
Fax: +49-89-8107-300<br>
neumann(a)snsb.de<br>
<br>
postal address:<br>
<br>
Bavarian Natural History Collections<br>
The Bavarian State Collection of Zoology<br>
Dirk Neumann, Section Ichthyology / DNA-Storage<br>
Muenchhausenstr. 21<br>
81247 Munich (Germany)<br>
<br>
Visit our section at:<br>
<a href="https://nam10.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.zsm.mwn.de%2Fsektion%2Fichthyologie-home%2F&data=02%7C01%7Cabentley%40ku.edu%7Cdf385ddaaca24707b7f808d844839aa4%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637334679345864489&sdata=oA0UQOnCYGez%2B4TQ99ItmIcksuL2fzMcv9xY%2FLd771A%3D&reserved=0">http://www.zsm.mwn.de/sektion/ichthyologie-home/</a><o:p></o:p></p>
</div>
</div>
</body>
</html>