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<div class="moz-cite-prefix">Dear Mandy & Greg,</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">in principle, collections handling
& storing unsorted malaise traps or ("unsorted") algae samples
already have EDNA collections (maybe without calling them so).
What Greg describes perfectly matches unsorted malaise trap
samples and I entirely agree with Greg and James that these
samples (and any individualised reference specimens that are
identified and isolated from those bulk samples) will have high
relevancy (and potential) for us in the future. <br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">As James pointed out, microbiome
research ("omics") is an important and fast developing new area
and Greg is absolutely right that traditional concepts to catalog
such samples wouldn't work. If you understand such samples as just
another kind of "multidimensional collection object" (such as for
example an insect drawer that holds <b><i>x</i></b> species from
<b><i>y </i></b>independent collection events and <b><i>z</i></b>
collections/collectors), management of such samples shouldn't be a
real challenge. The real challenges however could be the <i>preservation</i>
of such EDNA samples, especially of water-based EDNA samples from
marine or freshwater environments unless these samples are
maintained, i.e. cultivated like living algae collections (which
evolve and thus change over time).</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">And yes, absolutely agree with
questions on "regulatory compliance" that Greg raised - was
recently approached by a colleague how to handle a EDNA sample
that potentially contained DNA of an living fossil and highly
regulated CITES species. We surely need new concepts and ideas
here.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">All the best</div>
<div class="moz-cite-prefix">Dirk<br>
</div>
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<div class="moz-cite-prefix"><br>
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<div class="moz-cite-prefix"> <br>
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<div class="moz-cite-prefix"><br>
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<div class="moz-cite-prefix"><br>
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<div class="moz-cite-prefix">Am 24.08.2020 um 19:50 schrieb
Watkins-Colwell, Gregory:<br>
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<p class="MsoNormal">Ironically, this topic was recently brought
up at the ASIH Ichthyology and Herpetology Collections
Committee meeting. In short: yes… various institutions have
been approached to house such things and there seems
legitimately good reasons to house such samples in a natural
history collection. Some of the tricks are basically
artifacts of how museums “normally” do things. That is, we
normally store things pre-sorted and identified and tagged by
taxon and locality and cataloged into the appropriate
taxonomic division. In this case, most of that is unknown and
the data we have are primarily locality and time, without
taxon initially. Eventually somebody can say what taxa are in
that sample, but until then it is basically a tube of water,
or scat or dirt, etc. SO how then is it cataloged and in
which division? In fact, even after we know what’s in the
sample, how do we store it? Do we try to isolate each DNA
fragment by taxon (NO!!!!)… does the tube get cataloged by
every applicable division? Does the sample get subdivided so
that each division has a subsample of the same thing? So many
of the conversations are about details like that. There are
also some really fun practical issues such as, how do you
import/export it? Is it claimed as wildlife or just as
water? What happens when the water “becomes” wildlife (i.e.
you import water and export crocodile DNA that was in the
water all along)… does that impact any import/export
regulations, etc. Will wildlife agents assume you falsified
the initial report?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">In terms of regulatory compliance, would
some agencies say that a researcher need a CITES export permit
for water if it later turns out that it included DNA from
elephants or freshwater dolphins or whatever?
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Greg<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<p class="MsoNormal">****************<o:p></o:p></p>
<p class="MsoNormal">Gregory J. Watkins-Colwell<o:p></o:p></p>
<p class="MsoNormal">Sr. Collection Manager, Herpetology and
Ichthyology<o:p></o:p></p>
<p class="MsoNormal">Division of Vertebrate Zoology<o:p></o:p></p>
<p class="MsoNormal">Yale Peabody Museum of Natural History<o:p></o:p></p>
<p class="MsoNormal">Main Office: 203-432-3791; West Campus:
203-737-7568; Fax 203-432-9277<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Package shipping address:<o:p></o:p></p>
<p class="MsoNormal">Greg Watkins-Colwell<o:p></o:p></p>
<p class="MsoNormal">Division of Vertebrate Zoology<o:p></o:p></p>
<p class="MsoNormal">Yale Peabody Museum of Natural History<br>
170–210 Whitney Avenue<br>
New Haven, CT 06511 USA<o:p></o:p></p>
<p class="MsoNormal">203-432-3791<o:p></o:p></p>
<p class="MsoNormal">******************<o:p></o:p></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div style="border:none;border-top:solid #E1E1E1
1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b>From:</b> Nhcoll-l
<a class="moz-txt-link-rfc2396E" href="mailto:nhcoll-l-bounces@mailman.yale.edu"><nhcoll-l-bounces@mailman.yale.edu></a> <b>
On Behalf Of </b>Mandy Reid<br>
<b>Sent:</b> Sunday, August 23, 2020 9:29 PM<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] EDNA samples<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="EN-AU">I am wondering whether
any museums are accepting EDNA (Environmental DNA) samples,
such as water samples, animal scats etc., or whether you
have been approached regarding storing such samples in
museum collections?<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Is this something that
we should consider? It would be excellent if anyone is
interested in a discussion around this as it is something
Collections staff at the Australian Museum in Sydney have
been approached about.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Cheers<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Mandy<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal" style="line-height:150%"><b><span
style="font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif"
lang="EN-AU">Dr Mandy Reid</span></b><span
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lang="EN-AU">
<o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:150%"><span
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<o:p></o:p></span></p>
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