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<p class="MsoNormal">Something like that might work, if they are sturdy enough.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dr. Kelly M. Cassidy, Curator, Conner Museum<o:p></o:p></p>
<p class="MsoNormal">School of Biological Sciences<o:p></o:p></p>
<p class="MsoNormal">Box 644236<o:p></o:p></p>
<p class="MsoNormal">Washington State University<o:p></o:p></p>
<p class="MsoNormal">Pullman, WA 99164-4236<o:p></o:p></p>
<p class="MsoNormal">509-335-3515<o:p></o:p></p>
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<p class="MsoNormal"><b>From:</b> Pepijn Kamminga <pepijn.kamminga@naturalis.nl> <br>
<b>Sent:</b> Wednesday, January 20, 2021 8:06 AM<br>
<b>To:</b> Cassidy, Kelly Michela <cassidyk@wsu.edu><br>
<b>Cc:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] Techniques for taking toe pad samples?<o:p></o:p></p>
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<p class="MsoNormal">Dear dr. Cassidy,<o:p></o:p></p>
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<p class="MsoNormal">come to think of it, I have a tiny scissors in my toolkit, which has a very small blade (max 1 cm) and is in an angle relative to the handle, that gives you also more freedom of movement and has worked for me a few times with small toepads.
It look something like this:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif">Met vriendelijke groet / Kind regards,</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif">Pepijn Kamminga</span>
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<p class="MsoNormal"><span style="color:#222222">Senior Collection Manager Birds & Mammals</span><o:p></o:p></p>
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<p class="MsoNormal"><img width="160" height="82" style="width:1.6666in;height:.8583in" id="_x0000_i1028" src="https://06ecba7b-a-deac235a-s-sites.googlegroups.com/a/naturalis.nl/signatures/home/logo-new.png"><o:p></o:p></p>
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<sub><span style="font-family:"Arial",sans-serif">+31717519338 - - </span></sub><o:p></o:p></p>
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<p class="MsoNormal"><sub><span style="font-family:"Arial",sans-serif"><a href="mailto:pepijn.kamminga@naturalis.nl" target="_blank">pepijn.kamminga@naturalis.nl</a> - <a href="https://urldefense.com/v3/__https:/www.naturalis.nl__;!!JmPEgBY0HMszNaDT!4sKrxTOpJB8Ryjrl2ZRNhTw0XDIsgPaAUHdsk8bGnDG1AbkrHQsdH4nGV6CndIk$" target="_blank">www.naturalis.nl</a></span></sub><o:p></o:p></p>
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<p class="MsoNormal"><sub><span style="font-family:"Arial",sans-serif">Darwinweg 2, 2333 CR Leiden</span></sub><o:p></o:p></p>
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<p class="MsoNormal"><sub><span style="font-family:"Arial",sans-serif">Postbus 9517, 2300 RA Leiden</span></sub><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><a href="https://urldefense.com/v3/__https:/www.naturalis.nl/lang-leve__;!!JmPEgBY0HMszNaDT!4sKrxTOpJB8Ryjrl2ZRNhTw0XDIsgPaAUHdsk8bGnDG1AbkrHQsdH4nGwULrXHM$" target="_blank"><span style="text-decoration:none"><img border="0" width="250" height="140" style="width:2.6083in;height:1.4583in" id="_x0000_i1027" src="https://06ecba7b-a-deac235a-s-sites.googlegroups.com/a/naturalis.nl/signatures/home/schildpad.gif"></span></a><o:p></o:p></p>
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<p class="MsoNormal">Op wo 20 jan. 2021 om 16:55 schreef Cassidy, Kelly Michela <<a href="mailto:cassidyk@wsu.edu">cassidyk@wsu.edu</a>>:<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Thank you. I generally don’t have issues taking skin clips. It’s the toe pads that are a challenge to sample without putting stress on the study skin. I received another request
for toe pads (on kingfishers) this morning. Maybe some sort of tiny nippers would work better than a scalpel?<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Dr. Kelly M. Cassidy, Curator, Conner Museum<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">School of Biological Sciences<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Box 644236<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Washington State University<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Pullman, WA 99164-4236<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">509-335-3515<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><b>From:</b> Pepijn Kamminga <<a href="mailto:pepijn.kamminga@naturalis.nl" target="_blank">pepijn.kamminga@naturalis.nl</a>>
<br>
<b>Sent:</b> Wednesday, January 20, 2021 6:35 AM<br>
<b>To:</b> Cassidy, Kelly Michela <<a href="mailto:cassidyk@wsu.edu" target="_blank">cassidyk@wsu.edu</a>><br>
<b>Cc:</b> <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> Re: [Nhcoll-l] Techniques for taking toe pad samples?<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Dear dr. Cassidy,<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Similar requests are made for the bird and mammal collection of Naturalis Biodiversity Center, the Netherlands. Bird skins often have small flaps of skin that can be cut with fresh
scalpel blades. For me it helps to apply some counterpressure, but the blade should do the cutting, so I don't apply too much force. As for mammals I prefer to use specimens stored on alcohol, I make a small incision in the ventral side of the skin and take
a sample of the breast muscle. Mammalian toepads I find difficult in general. If the requested specimen is a skin, cutting a piece of tissue along the seam can also do the trick. I hope this is of any help to you.<br clear="all">
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-family:"Arial",sans-serif">Met vriendelijke groet / Kind regards,</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-family:"Arial",sans-serif">Pepijn Kamminga</span>
<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Senior Collection Manager Birds & Mammals <o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><img border="0" width="160" height="82" style="width:1.6666in;height:.8583in" id="_x0000_i1026" src="https://06ecba7b-a-deac235a-s-sites.googlegroups.com/a/naturalis.nl/signatures/home/logo-new.png"><o:p></o:p></p>
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<sub><span style="font-family:"Arial",sans-serif">+31717519338 - - </span></sub><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><sub><span style="font-family:"Arial",sans-serif"><a href="mailto:pepijn.kamminga@naturalis.nl" target="_blank">pepijn.kamminga@naturalis.nl</a> - <a href="https://urldefense.com/v3/__https:/www.naturalis.nl__;!!JmPEgBY0HMszNaDT!-MEoFpA5aTMtbFqkZxBhK2QCzCz_yq7CPSVoQwY7JXE1dZe0iCzcOb7empd--KQ$" target="_blank">www.naturalis.nl</a></span></sub><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><sub><span style="font-family:"Arial",sans-serif">Darwinweg 2, 2333 CR Leiden</span></sub><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><sub><span style="font-family:"Arial",sans-serif">Postbus 9517, 2300 RA Leiden</span></sub><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><a href="https://urldefense.com/v3/__https:/www.naturalis.nl/lang-leve__;!!JmPEgBY0HMszNaDT!-MEoFpA5aTMtbFqkZxBhK2QCzCz_yq7CPSVoQwY7JXE1dZe0iCzcOb7ePk4zTL8$" target="_blank"><span style="text-decoration:none"><img border="0" width="250" height="140" style="width:2.6in;height:1.4583in" id="_x0000_i1025" src="https://06ecba7b-a-deac235a-s-sites.googlegroups.com/a/naturalis.nl/signatures/home/schildpad.gif"></span></a><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Op di 19 jan. 2021 om 19:27 schreef Cassidy, Kelly Michela <<a href="mailto:cassidyk@wsu.edu" target="_blank">cassidyk@wsu.edu</a>>:<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I am getting more frequent requests for toe pad samples for DNA sequencing, instead of skin clips.
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">I find it difficult to take these samples without putting stress on the prepared skin. The toe pads are tough and leathery. Scalpels don’t cut into them very easily, if at all.
The most recent request is for a sample from a vole, which has small feet.<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Are there any techniques or recommended tools to help minimize stress and possible damage to skins when taking toe pad samples?
<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Dr. Kelly M. Cassidy, Curator, Conner Museum<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">School of Biological Sciences<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Box 644236<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Washington State University<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Pullman, WA 99164-4236<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">509-335-3515<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
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