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<p class="MsoNormal">Hi Paul,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">An interesting question and one that has left many of us puzzled. I fret over what to do with a dry crab collected by the Exploring Expedition – it is the type of the famously eaten Dungeness crab, and the carapace is delaminating. I
found the crab in a glassware cabinet in a superheated basement hallway; why this precious specimen was there……..<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Anyway, I have had excellent success rehydrating long dried smaller crustacea, amphipods, isopods, etc. using Aerosol-OT, something Bill Moser suggested we use for dried leeches. See the link following for an example. It was the dried
syntype of Hyalella dentata from the 19<sup>th</sup> century. I rehydrated it in Aerosol OT, and then was able to dissect it and make wonderful slides. If you follow the link you will see the pre-hydrating image of the whole specimen, and then scrolling
down to the bottom you can see the thumbnails of slide images to get a sense of the quality.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Also, Les Watling at U. Hawaii raved about another surfactant (dioctyl sodium sulfosuccinate) that is mentioned in a paper by Paul Jeppensen. See link:
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><a href="https://www.jstor.org/stable/20104402?seq=1#metadata_info_tab_contents">https://www.jstor.org/stable/20104402?seq=1#metadata_info_tab_contents</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best, Eric<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Eric A. Lazo-Wasem<o:p></o:p></p>
<p class="MsoNormal">Senior Collections Manager<o:p></o:p></p>
<p class="MsoNormal">Peabody Museum of Natural History<o:p></o:p></p>
<p class="MsoNormal">Yale University<o:p></o:p></p>
<p class="MsoNormal">170 Whitney Ave.<o:p></o:p></p>
<p class="MsoNormal">New Haven, CT 06520<o:p></o:p></p>
<p class="MsoNormal">203 432-3784<o:p></o:p></p>
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<p class="MsoNormal"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> <b>
On Behalf Of </b>Callomon,Paul<br>
<b>Sent:</b> Wednesday, May 5, 2021 10:35 AM<br>
<b>To:</b> NH-COLL listserv (nhcoll-l@mailman.yale.edu) <nhcoll-l@mailman.yale.edu><br>
<b>Subject:</b> [Nhcoll-l] Hydrating old crustaceans<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Folks,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I’m doing some research and testing to assess whether our very old dry crustacean specimens would be better placed in fluid from now. Left in air it seems inevitable that they will eventually dry out to the point of disintegration. I’d
like to solicit people’s input on these two points:<o:p></o:p></p>
<p class="MsoNormal">- Reviewing the literature on this subject, particularly accounts of actual hydration/fluidization protocols and experiments<o:p></o:p></p>
<p class="MsoNormal">- Designing experiments and publishing basic procedures<o:p></o:p></p>
<p class="MsoNormal">If you know of relevant publications, have anecdotal experience of your own and/or would be interested in joining an informal working group on this topic, please email me directly.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">Paul Callomon<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">Collection Manager, Malacology and General Invertebrates<i><o:p></o:p></i></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:12.0pt;color:black">Academy of Natural Sciences of Drexel University<o:p></o:p></span></b></p>
<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA<br>
<i><a href="mailto:prc44@drexel.edu">prc44@drexel.edu</a> Tel 215-405-5096 - Fax 215-299-1170</i><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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