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<p class="MsoNormal">Hi Simon,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Aerosol OT is a commercial surfactant manufactured by Ricca Chemmical Company in the US (Texas). We buy it through a scientific supply company. From the ingredients list I see it contains various alcohols, and “Dicosate Sodium.” It is
a very slippery solution and can take some time to work. I usually toss specimens in and let them sit about a week; sometimes I forget and they stay for a month or more and I have seen no degradation as a result. For recently dry specimens (polychaetes dried
out over Covid “break”) the critters were in good shape in a few days. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Previously, I used with some success tri-basic calcium phosphate, a bit of a variation on TSP that I learned of from the former curator many years ago.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best, Eric<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Eric A. Lazo-Wasem<o:p></o:p></p>
<p class="MsoNormal">Senior Collections Manager<o:p></o:p></p>
<p class="MsoNormal">Peabody Museum of Natural History<o:p></o:p></p>
<p class="MsoNormal">Yale University<o:p></o:p></p>
<p class="MsoNormal">170 Whitney Ave.<o:p></o:p></p>
<p class="MsoNormal">New Haven, CT 06520<o:p></o:p></p>
<p class="MsoNormal">203 432-3784<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Simon Moore <couteaufin@btinternet.com> <br>
<b>Sent:</b> Wednesday, May 5, 2021 12:25 PM<br>
<b>To:</b> Lazo-Wasem, Eric <eric.lazo-wasem@yale.edu><br>
<b>Cc:</b> Callomon,Paul <prc44@drexel.edu>; NHCOLL-new <nhcoll-l@mailman.yale.edu><br>
<b>Subject:</b> Re: [Nhcoll-l] Hydrating old crustaceans<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks Eric and Paul, for bringing up a subject that has always been a bit of a challenge as ‘crusties’ are one of the most difficult animals to rehydrate successfully. I remember the Jeppesen paper when it came out and it steered me away
from the tried but not-always-successful tri-sodium phosphate solution of 1947 into using the lab surfactant Decon-90 at 3 to 5% and this has proved very successful overall, especially for high protein organisms. Di-octyl Sulpho-succinate is also very effective.
However, Eric mentioned Aerosol OT which is unknown to me, so Eric, please could we have some more details? The link you kindly attached, sent sent me to Jeppesen’s paper which I cannot download as I’m not part of a library, university &c.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">With all good wishes, Simon<br>
<br>
Simon Moore MIScT, RSci, FLS, ACR<br>
Conservator of Natural Sciences and Cutlery Historian,</span><br>
<br>
<a href="https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.natural-history-conservation.com%2F&data=04%7C01%7Ceric.lazo-wasem%40yale.edu%7Cfe21c57ee16c4a47dd3608d90fe262d2%7Cdd8cbebb21394df8b4114e3e87abeb5c%7C0%7C0%7C637558287269231292%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=GvFQ1Vw9PhR8rbVdympo5u0X0An2TWb9od%2BeIJciiMg%3D&reserved=0">www.natural-history-conservation.com</a><br>
<br>
<br>
<img border="0" width="240" height="100" style="width:2.5in;height:1.0416in" id="_x0039_C08ABF2-7AFB-4F47-A725-E5AF8049277A" src="cid:image001.png@01D741AD.3468F3C0"><img border="0" width="394" height="90" style="width:4.1041in;height:.9375in" id="A9D8EAF0-C632-4B39-A7BB-A28157F131CE" src="cid:image002.jpg@01D741AD.3468F3C0"><o:p></o:p></p>
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<br>
<o:p></o:p></p>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">On 5 May 2021, at 16:37, Lazo-Wasem, Eric <<a href="mailto:eric.lazo-wasem@yale.edu">eric.lazo-wasem@yale.edu</a>> wrote:<br>
<br>
Hi Paul,<br>
<br>
An interesting question and one that has left many of us puzzled. I fret over what to do with a dry crab collected by the Exploring Expedition – it is the type of the famously eaten Dungeness crab, and the carapace is delaminating. I found the crab in a glassware
cabinet in a superheated basement hallway; why this precious specimen was there……..<br>
<br>
Anyway, I have had excellent success rehydrating long dried smaller crustacea, amphipods, isopods, etc. using Aerosol-OT, something Bill Moser suggested we use for dried leeches. See the link following for an example. It was the dried syntype of Hyalella
dentata from the 19th century. I rehydrated it in Aerosol OT, and then was able to dissect it and make wonderful slides. If you follow the link you will see the pre-hydrating image of the whole specimen, and then scrolling down to the bottom you can see
the thumbnails of slide images to get a sense of the quality. <br>
<br>
Also, Les Watling at U. Hawaii raved about another surfactant (dioctyl sodium sulfosuccinate) that is mentioned in a paper by Paul Jeppensen. See link:<br>
<br>
<a href="https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jstor.org%2Fstable%2F20104402%3Fseq%3D1%23metadata_info_tab_contents&data=04%7C01%7Ceric.lazo-wasem%40yale.edu%7Cfe21c57ee16c4a47dd3608d90fe262d2%7Cdd8cbebb21394df8b4114e3e87abeb5c%7C0%7C0%7C637558287269241271%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=VjWMLrsX23lWWqgnajVWF3c8tGnLQaDYlnr9F1EGW7M%3D&reserved=0">https://www.jstor.org/stable/20104402?seq=1#metadata_info_tab_contents</a><br>
<br>
Best, Eric<br>
<br>
<br>
<br>
Eric A. Lazo-Wasem<br>
Senior Collections Manager<br>
Peabody Museum of Natural History<br>
Yale University<br>
170 Whitney Ave.<br>
New Haven, CT 06520<br>
203 432-3784<br>
<br>
From: Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a>> On Behalf Of Callomon,Paul<br>
Sent: Wednesday, May 5, 2021 10:35 AM<br>
To: NH-COLL listserv (<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a>) <<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a>><br>
Subject: [Nhcoll-l] Hydrating old crustaceans<br>
<br>
Folks,<br>
<br>
I’m doing some research and testing to assess whether our very old dry crustacean specimens would be better placed in fluid from now. Left in air it seems inevitable that they will eventually dry out to the point of disintegration. I’d like to solicit people’s
input on these two points:<br>
- Reviewing the literature on this subject, particularly accounts of actual hydration/fluidization protocols and experiments<br>
- Designing experiments and publishing basic procedures<br>
If you know of relevant publications, have anecdotal experience of your own and/or would be interested in joining an informal working group on this topic, please email me directly.<br>
<br>
Paul Callomon<br>
Collection Manager, Malacology and General Invertebrates<br>
Academy of Natural Sciences of Drexel University<br>
1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA<br>
<a href="mailto:prc44@drexel.edu">prc44@drexel.edu</a> Tel 215-405-5096 - Fax 215-299-1170<br>
<br>
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