<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
</head>
<body>
<div class="moz-cite-prefix">Dear Sergio,</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">alone in the Eu there are over 30
accredited denaturants, often they are mixed to make the ethanol
undrinkable. While some have limited effects on the stability of
the DNA-molecule, others do affect the integrity sooner or later.
This is a gradual process that is strongly linked with the
polarity of the denaturing agents, causing fragmentation and
degradation of the DNA.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Usually, especially in commercial
product, it is not really specified which denaturants exactly have
been added, and even though you use the same product, denaturing
agents may change. Since 2012, the EU strives to harmonise
taxation of ethanol and thus spoiling of ethanol to make it
undrinkable. More information is provided here:
<a class="moz-txt-link-freetext" href="https://ec.europa.eu/taxation_customs/business/excise-duties-alcohol-tobacco-energy/excise-duties-alcohol/alcohol-not-human-consumption_en">https://ec.europa.eu/taxation_customs/business/excise-duties-alcohol-tobacco-energy/excise-duties-alcohol/alcohol-not-human-consumption_en</a></div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">The results can be found here:
<a class="moz-txt-link-freetext" href="https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32018R1880&from=EN">https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32018R1880&from=EN</a></div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix"><i><span style="left: 137.101px; top:
889.469px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.964814);" dir="ltr">The common </span></i><i><span
style="left: 233.195px; top: 889.469px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.968978);"
dir="ltr">denatur</span></i><i><span style="left: 286.108px;
top: 889.469px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.967178);" dir="ltr">ing procedure </span></i><i><span
style="left: 386.832px; top: 889.469px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.998983);"
dir="ltr">for completely </span></i><i><span style="left:
492.563px; top: 889.469px; font-size: 15.9925px; font-family:
sans-serif; transform: scaleX(0.939699);" dir="ltr">denatured
</span></i><i><span style="left: 566.171px; top: 889.469px;
font-size: 15.9925px; font-family: sans-serif; transform:
scaleX(0.95288);" dir="ltr">alcohol </span></i><i><span
style="left: 620.5px; top: 889.469px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.965706);"
dir="ltr">employed in Belgium, </span></i><i><span
style="left: 774.326px; top: 889.469px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.925781);"
dir="ltr">Bulgaria, Czech </span></i><i><span style="left:
137.101px; top: 907.045px; font-size: 15.9925px; font-family:
sans-serif; transform: scaleX(0.940444);" dir="ltr">Republic,
</span></i><i><span style="left: 210.235px; top: 907.045px;
font-size: 15.9925px; font-family: sans-serif; transform:
scaleX(0.952184);" dir="ltr">Denmark, </span></i><i><span
style="left: 286.865px; top: 907.045px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.940154);"
dir="ltr">Germany, Estonia, </span></i><i><span style="left:
424.91px; top: 907.045px; font-size: 15.9925px; font-family:
sans-serif; transform: scaleX(0.945447);" dir="ltr">Ireland, </span></i><i><span
style="left: 485.76px; top: 907.045px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.880868);"
dir="ltr">Greece, </span></i><i><span style="left: 546.044px;
top: 907.045px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.892776);" dir="ltr">Spain, </span></i><i><span
style="left: 596.689px; top: 907.045px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.878988);"
dir="ltr">France, </span></i><i><span style="left: 654.516px;
top: 907.045px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.970042);" dir="ltr">Italy, Cyprus, Latvia,
</span></i><i><span style="left: 813.16px; top: 907.045px;
font-size: 15.9925px; font-family: sans-serif; transform:
scaleX(0.94634);" dir="ltr">Lithuania, </span></i><i><span
style="left: 137.101px; top: 924.713px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.948847);"
dir="ltr">Luxembourg, </span></i><i><span style="left:
233.384px; top: 924.713px; font-size: 15.9925px; font-family:
sans-serif; transform: scaleX(0.963511);" dir="ltr">Hungar</span></i><i><span
style="left: 284.22px; top: 924.713px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.902287);"
dir="ltr">y, Malta, </span></i><i><span style="left:
349.32px; top: 924.713px; font-size: 15.9925px; font-family:
sans-serif; transform: scaleX(0.959498);" dir="ltr">the
Netherlands, </span></i><i><span style="left: 469.697px; top:
924.713px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.929656);" dir="ltr">Austria, Poland, </span></i><i><span
style="left: 586.39px; top: 924.713px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.920019);"
dir="ltr">Portugal, Romania, </span></i><i><span style="left:
724.058px; top: 924.713px; font-size: 15.9925px; font-family:
sans-serif; transform: scaleX(0.907502);" dir="ltr">Slovenia,
Slovakia and </span></i><i><span style="left: 137.101px; top:
942.288px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.928356);" dir="ltr">Finland: </span></i><i><span
style="left: 137.101px; top: 981.216px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.888728);"
dir="ltr">Per hectolit</span></i><i><span style="left:
208.062px; top: 981.216px; font-size: 15.9925px; font-family:
sans-serif; transform: scaleX(0.883193);" dir="ltr">re of
absolute </span></i><span style="left: 299.054px; top:
981.216px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.871774);" dir="ltr"><i>ethanol:</i> <br>
</span></div>
<div class="moz-cite-prefix"><span style="left: 299.054px; top:
981.216px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.871774);" dir="ltr"></span><span
style="left: 137.101px; top: 1012.96px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.939928);" dir="ltr">—
1,0 litre isoprop</span><span style="left: 263.053px; top:
1012.96px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.907405);" dir="ltr">yl alcohol, </span><span
style="left: 137.101px; top: 1044.81px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.919844);" dir="ltr"><br>
</span></div>
<div class="moz-cite-prefix"><span style="left: 137.101px; top:
1044.81px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.919844);" dir="ltr">— 1,0 litre methyl ethyl
ketone, </span><span style="left: 137.101px; top: 1076.65px;
font-size: 15.9925px; font-family: sans-serif; transform:
scaleX(0.909839);" dir="ltr"><br>
</span></div>
<div class="moz-cite-prefix"><span style="left: 137.101px; top:
1076.65px; font-size: 15.9925px; font-family: sans-serif;
transform: scaleX(0.909839);" dir="ltr">— 1,0 gram denaton</span><span
style="left: 273.73px; top: 1076.65px; font-size: 15.9925px;
font-family: sans-serif; transform: scaleX(0.876148);" dir="ltr">ium
benzoate. </span></div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Isoprop and Bitrex (denatonium
benzoate) surely are not an issue, but the keton MEK breaks down
over time as it is a highly polar molecule that tends to oxidise
other (bio) molecules.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">While some EU-countries allow research
institutions the usage of untaxed undentaured ethanol, other
countries don't. And even though a common procedure for denaturing
has been approved, you have no control that it is always applied.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Thus I would clearly vote for exclusive
usage of undenatured, pure (96%) EtOH. It might be worth noting
negative effects on the DNA-quality has been reported from 99.8%
EtOH occasionally, which might be linked with residual chemical
agents (usually low boiling benzines) used to "dry" the ethanol
above 96%.</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Hope this helps</div>
<div class="moz-cite-prefix">Dirk <br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Am 13.05.2021 um 11:06 schrieb Sergio
Montagud:<br>
</div>
<blockquote type="cite"
cite="mid:58B70818-F883-4A28-BF4F-C08A31EE38C2@gmail.com">
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
<meta name="Generator" content="Microsoft Word 15 (filtered
medium)">
<style>@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0cm;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}p.msonormal0, li.msonormal0, div.msonormal0
{mso-style-name:msonormal;
mso-margin-top-alt:auto;
margin-right:0cm;
mso-margin-bottom-alt:auto;
margin-left:0cm;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}span.EmailStyle18
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}span.apple-converted-space
{mso-style-name:apple-converted-space;}span.EmailStyle20
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:#1F497D;}span.EmailStyle21
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}div.WordSection1
{page:WordSection1;}</style>
<div class="WordSection1">
<p class="MsoNormal"><span lang="EN-US">No, I’m referring to
ethanol or ethyl alcohol.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Sergio<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<div style="border:none;border-top:solid #B5C4DF
1.0pt;padding:3.0pt 0cm 0cm 0cm">
<p class="MsoNormal"><b><span
style="font-size:12.0pt;color:black">From: </span></b><span
style="font-size:12.0pt;color:black">Lennart Lennuk
<a class="moz-txt-link-rfc2396E" href="mailto:Lennart.Lennuk@loodusmuuseum.ee"><Lennart.Lennuk@loodusmuuseum.ee></a><br>
<b>Date: </b>Thursday, 13 May 2021 at 11:00<br>
<b>To: </b>Sergio Montagud
<a class="moz-txt-link-rfc2396E" href="mailto:sergio.montagud@gmail.com"><sergio.montagud@gmail.com></a>,
<a class="moz-txt-link-rfc2396E" href="mailto:nhcoll-l@mailman.yale.edu">"nhcoll-l@mailman.yale.edu"</a>
<a class="moz-txt-link-rfc2396E" href="mailto:nhcoll-l@mailman.yale.edu"><nhcoll-l@mailman.yale.edu></a><br>
<b>Subject: </b>RE: [Nhcoll-l] FW: Alcohol concentration
for terrestrial vertebrates<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<p class="MsoNormal"><span style="color:#1F497D">Are you talking
about methanol?</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D"> </span><o:p></o:p></p>
<div>
<div style="border:none;border-top:solid #E1E1E1
1.0pt;padding:3.0pt 0cm 0cm 0cm">
<p class="MsoNormal"><b>From:</b> Nhcoll-l
[<a class="moz-txt-link-freetext" href="mailto:nhcoll-l-bounces@mailman.yale.edu">mailto:nhcoll-l-bounces@mailman.yale.edu</a>] <b>On Behalf
Of </b>Sergio Montagud<br>
<b>Sent:</b> Thursday, May 13, 2021 11:59 AM<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] FW: Alcohol concentration for
terrestrial vertebrates<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"><span style="color:black" lang="EN-US">I
take advantage of this interesting conversation to ask about
properties to preserve DNA of commercial ethanol (96 %) used
for sanitary use. I have heard different opinions. A lot of
them says that the component used in this ethanol to make it
undrinkable could affect the DNA stabilization. Other people
employ it to preserve the samples and seems not to have
problems about DNA extractions. After years of experiences…
Does someone knows if there is any work or article that
definitively concludes on this?</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black" lang="EN-US"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black" lang="EN-US">Sergio</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black">--</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black">********************************</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black">Sergio Montagud
Alario</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black">Museu [UV]
Història Natural</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black">Universitat de
València</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black">e-mail: <a
href="mailto:sergio.montagud@uv.es" moz-do-not-send="true"><span
style="color:purple">sergio.montagud@uv.es</span></a></span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black" lang="EN-US">********************************</span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
</div>
<br>
<fieldset class="mimeAttachmentHeader"></fieldset>
<pre class="moz-quote-pre" wrap="">_______________________________________________
Nhcoll-l mailing list
<a class="moz-txt-link-abbreviated" href="mailto:Nhcoll-l@mailman.yale.edu">Nhcoll-l@mailman.yale.edu</a>
<a class="moz-txt-link-freetext" href="https://mailman.yale.edu/mailman/listinfo/nhcoll-l">https://mailman.yale.edu/mailman/listinfo/nhcoll-l</a>
_______________________________________________
NHCOLL-L is brought to you by the Society for the Preservation of
Natural History Collections (SPNHC), an international society whose
mission is to improve the preservation, conservation and management of
natural history collections to ensure their continuing value to
society. See <a class="moz-txt-link-freetext" href="http://www.spnhc.org">http://www.spnhc.org</a> for membership information.
Advertising on NH-COLL-L is inappropriate.
</pre>
</blockquote>
<p><br>
</p>
<div class="moz-signature">-- <br>
<p><img src="cid:part2.A1619630.0D3EAF9A@snsb.de" alt=""
width="152" height="59"></p>
<p><br>
Dirk Neumann<br>
<br>
Tel: 089 / 8107-111<br>
Fax: 089 / 8107-300<br>
neumann(a)snsb.de<br>
<br>
Postanschrift:<br>
<br>
Staatliche Naturwissenschaftliche Sammlungen Bayerns<br>
Zoologische Staatssammlung München<br>
Dirk Neumann, Sektion Ichthyologie / DNA-Storage<br>
Münchhausenstr. 21<br>
81247 München<br>
<br>
Besuchen Sie unsere Sammlung:<br>
<a class="moz-txt-link-freetext" href="http://www.zsm.mwn.de/sektion/ichthyologie-home/">http://www.zsm.mwn.de/sektion/ichthyologie-home/</a><br>
<br>
---------<br>
<br>
Dirk Neumann<br>
<br>
Tel: +49-89-8107-111<br>
Fax: +49-89-8107-300<br>
neumann(a)snsb.de<br>
<br>
postal address:<br>
<br>
Bavarian Natural History Collections<br>
The Bavarian State Collection of Zoology<br>
Dirk Neumann, Section Ichthyology / DNA-Storage<br>
Muenchhausenstr. 21<br>
81247 Munich (Germany)<br>
<br>
Visit our section at:<br>
<a class="moz-txt-link-freetext" href="http://www.zsm.mwn.de/sektion/ichthyologie-home/">http://www.zsm.mwn.de/sektion/ichthyologie-home/</a><br>
<br>
</p>
</div>
</body>
</html>