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<p class="MsoNormal">Hi Joosep,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">96% Ethanol (with no additives) will actually be better for DNA preservation as it effectively dehydrates the specimen and reduces hydrolytic effects. So I’d keep them in the 96% EtOH. ‘Cold and dry’ tends to be best for biomolecule preservation.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">As to the denatured EtOH – then probably best to transfer into equivalent absolute ethanol. The ‘denaturant’ used can be very variable though usually something like MEK or Methanol, both of which are better avoided with DNA samples.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Hope that helps.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">All the best,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Jules<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB">Julian Carter (he/him)<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB">Prif Gadwraethydd, Gwyddorau Naturiol / Principal Conservator Natural Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"><a href="mailto:julian.carter@amgueddfacymru.ac.uk">julian.carter@amgueddfacymru.ac.uk</a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"><a href="mailto:julian.carter@museumwales.ac.uk">julian.carter@museumwales.ac.uk</a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"><a href="https://www.researchgate.net/profile/Julian_Carter">https://www.researchgate.net/profile/Julian_Carter</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">Twitter: @NatHistConserve
</span><span lang="CY" style="mso-fareast-language:EN-GB"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB">Ffon/Tel: (+44) 029 20573230 / (+44)07870448074<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="mso-fareast-language:EN-GB">From:</span></b><span lang="EN-US" style="mso-fareast-language:EN-GB"> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu>
<b>On Behalf Of </b>Joosep Sarapuu<br>
<b>Sent:</b> 20 May 2021 14:12<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] Fluid preservation correction<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="ET">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="ET"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="ET">I have a question about fluid preservation correction.
<o:p></o:p></span></p>
<ol style="margin-top:0cm" start="1" type="1">
<li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo2"><span lang="ET">I have three samples of animals in 96% EtOH right now, but i know that the best for DNA would be 75% EtOH. Can i just dilute this to the right amount or i have to wash
it in destilled water and then step by step to 75% EtOH?<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0cm;mso-list:l0 level1 lfo2"><span lang="ET">I have one animal in denaturated EtOH. This one i have to wash and then also step by step to 75% normal EtOH?<o:p></o:p></span></li></ol>
<p class="MsoNormal"><span lang="ET"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="ET"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="ET" style="mso-fareast-language:ET">Sincerely,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="ET" style="mso-fareast-language:ET">Joosep Sarapuu<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="ET"><o:p> </o:p></span></p>
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