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<p class="MsoNormal"><a name="_GoBack"></a>Julian<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">That is presuming that the specimens were not first fixed in formalin before being placed in 95% ethanol.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Andy<o:p></o:p></p>
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<p class="MsoNormal"><span style="color:black">Andy Bentley<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Ichthyology Collection Manager<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> on behalf of "Julian uk>" <Julian.Carter@museumwales.ac.uk><br>
<b>Date: </b>Thursday, May 20, 2021 at 1:51 PM<br>
<b>To: </b>Joosep Sarapuu <Joosep.Sarapuu@loodusmuuseum.ee>, "nhcoll-l@mailman.yale.edu" <nhcoll-l@mailman.yale.edu><br>
<b>Subject: </b>Re: [Nhcoll-l] Fluid preservation correction<o:p></o:p></span></p>
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<p class="MsoNormal">Hi Joosep,<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">96% Ethanol (with no additives) will actually be better for DNA preservation as it effectively dehydrates the specimen and reduces hydrolytic effects. So I’d keep them in the 96% EtOH. ‘Cold and dry’ tends to be best for biomolecule preservation.<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">As to the denatured EtOH – then probably best to transfer into equivalent absolute ethanol. The ‘denaturant’ used can be very variable though usually something like MEK or Methanol, both of which are better avoided with DNA samples.<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">Hope that helps.<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">All the best,<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">Jules<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB">Julian Carter (he/him)</span><o:p></o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB">Prif Gadwraethydd, Gwyddorau Naturiol / Principal Conservator Natural Sciences</span><o:p></o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"><a href="mailto:julian.carter@amgueddfacymru.ac.uk">julian.carter@amgueddfacymru.ac.uk</a></span><o:p></o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"><a href="mailto:julian.carter@museumwales.ac.uk">julian.carter@museumwales.ac.uk</a></span><o:p></o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"><a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.researchgate.net%2Fprofile%2FJulian_Carter&data=04%7C01%7Cabentley%40ku.edu%7C9cef566e7936499ec61408d91bc03c07%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637571334730590915%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=WCKxq29hI2Ktzo794XHR0SvqvlZfdiV2NTWSfG1AQuk%3D&reserved=0">https://www.researchgate.net/profile/Julian_Carter</a></span><o:p></o:p></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">Twitter: @NatHistConserve
</span><o:p></o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB">Ffon/Tel: (+44) 029 20573230 / (+44)07870448074</span><o:p></o:p></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB"> </span><o:p></o:p></p>
<p class="MsoNormal"><span lang="CY" style="mso-fareast-language:EN-GB"> </span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal"><b><span style="mso-fareast-language:EN-GB">From:</span></b><span style="mso-fareast-language:EN-GB"> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu>
<b>On Behalf Of </b>Joosep Sarapuu<br>
<b>Sent:</b> 20 May 2021 14:12<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] Fluid preservation correction</span><o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"><span lang="ET">Dear all,</span><o:p></o:p></p>
<p class="MsoNormal"><span lang="ET"> </span><o:p></o:p></p>
<p class="MsoNormal"><span lang="ET">I have a question about fluid preservation correction.
</span><o:p></o:p></p>
<ol style="margin-top:0in" start="1" type="1">
<li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo3"><span lang="ET">I have three samples of animals in 96% EtOH right now, but i know that the best for DNA would be 75% EtOH. Can i just dilute this to the right amount or i have to wash
it in destilled water and then step by step to 75% EtOH?</span><o:p></o:p></li><li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo3"><span lang="ET">I have one animal in denaturated EtOH. This one i have to wash and then also step by step to 75% normal EtOH?</span><o:p></o:p></li></ol>
<p class="MsoNormal"><span lang="ET"> </span><o:p></o:p></p>
<p class="MsoNormal"><span lang="ET"> </span><o:p></o:p></p>
<p class="MsoNormal"><span lang="ET" style="mso-fareast-language:ET">Sincerely,</span><o:p></o:p></p>
<p class="MsoNormal"><span lang="ET" style="mso-fareast-language:ET">Joosep Sarapuu</span><o:p></o:p></p>
<p class="MsoNormal"><span lang="ET"> </span><o:p></o:p></p>
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