<div dir="auto">Hi Nicole,<div dir="auto">Check our recent paper. It seems to be a similar situation</div><div dir="auto"><a href="https://zookeys.pensoft.net/articles.php?id=64607&journal_name=zookeys">https://zookeys.pensoft.net/articles.php?id=64607&journal_name=zookeys</a><br></div><div dir="auto">Best!</div><div dir="auto">Luis</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">A sexta, 20/08/2021, 21:37, Bentley, Andrew Charles <<a href="mailto:abentley@ku.edu">abentley@ku.edu</a>> escreveu:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d">Nicole<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d">I would not rinse in water of any kind as this may pose a risk of osmotic changes in the specimens. I would presume they are in some kind of alcohol unless a
formalin test strip proves otherwise and would transfer into new preservative as is without any washing.<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d">Andy<u></u><u></u></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" target="_blank" rel="noreferrer">nhcoll-l-bounces@mailman.yale.edu</a>>
<b>On Behalf Of </b>Nicole Seiden<br>
<b>Sent:</b> Friday, August 20, 2021 3:25 PM<br>
<b>To:</b> NHCOLL-new <<a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank" rel="noreferrer">nhcoll-l@mailman.yale.edu</a>><br>
<b>Subject:</b> [Nhcoll-l] How to transfer specimens out of an unknown preservative?<u></u><u></u></span></p>
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<span style="font-size:11.0pt;line-height:106%;font-family:"Calibri",sans-serif;color:black">Hello and happy Friday! <u></u><u></u></span></p>
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<span style="font-size:11.0pt;line-height:106%;font-family:"Calibri",sans-serif;color:black">I was recently hired as a collections manager with the initial tasks of digitizing and re-opening the collections. The collections have been “ignored” for some time
now and both the specimens and the database have numerous discrepancies that I’ve been addressing. Currently, I’m working with our wet-preserved specimens and want to ask the community for some advice. <u></u><u></u></span></p>
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<span style="font-size:11.0pt;line-height:106%;font-family:"Calibri",sans-serif;color:black">A large portion of our specimens (> 4,000) are preserved in an unknown fluid (listed as N/A), and many of which require topping up or rehousing to larger jars. Our
most common preservative is ethanol, and it’s very likely that most are in ethanol or isopropyl, though I have had the unpleasant misfortune of opening a few preserved in formalin. <u></u><u></u></span></p>
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<span style="font-size:11.0pt;line-height:106%;font-family:"Calibri",sans-serif;color:black">Previous discussions indicated there is not a method to determine the fluid type, but is there a way to transfer the specimens into a known preservative? My initial
thought is to rinse (or soak?) them in DI water and then transfer into ethanol. Does this pose any risk to the specimens (i.e., lead to damage)? Are there any precautions I should follow? <u></u><u></u></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri",sans-serif;color:black">Many thanks in advanced, <u></u><u></u></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri",sans-serif;color:black">Nicki<u></u><u></u></span></p>
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<p style="background:white"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#174e86">Nicki L. Seiden, M.Sc.</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><u></u><u></u></span></p>
<p style="background:white;text-align:start"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#174e86">She/Her/Hers</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><u></u><u></u></span></p>
<p style="background:white;text-align:start"><i><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#174e86">Research Collection Manager</span></i><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><u></u><u></u></span></p>
<p style="background:white;text-align:start"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#174e86">Harbor Branch Oceanographic Institute</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><u></u><u></u></span></p>
<p style="background:white;text-align:start"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><a href="mailto:hmcqueen@fau.edu" target="_blank" rel="noreferrer"><span style="color:#0563c1">nseiden@fau.edu</span></a><u></u><u></u></span></p>
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