<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
<style type="text/css" style="display:none;"> P {margin-top:0;margin-bottom:0;} </style>
</head>
<body dir="ltr">
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
Greetings Lennart,</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
I recommend taking a look at the SPNHC wiki for guidance on collecting and storing tissue samples:</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<a href="https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection" id="LPlnk641881">https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection</a><br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<a href="https://spnhc.biowikifarm.net/wiki/Genetic_Resources" id="LPlnk959084">https://spnhc.biowikifarm.net/wiki/Genetic_Resources</a><br>
</div>
<div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
But in short, we use 2ml externally threaded cryovials for tissue samples (many brands available from lab suppliers). Using 95% ETOH is an effective preservative and can be stored in a regular freezer if you don't have access to ultracold freezing or liquid
nitrogen. Volume of tissue harvested is up to your institution based on anticipated usage, space, and species rarity. We tend to take three vials (heart, muscle, liver), but many institutions combine these samples into one vial, while others may sample additional
organs. If we receive a rare or endangered species, we tend to increase our sampling.</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
Best,<br>
Emily</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<br>
</div>
<div id="Signature">
<div>
<div id="divtagdefaultwrapper" dir="ltr" style="font-size:12pt; color:#000000; font-family:Calibri,Arial,Helvetica,sans-serif">
<p style="margin-top: 0px; margin-bottom: 0px;"><span id="ms-rterangepaste-start"></span></p>
<div style="color:rgb(33,33,33); font-size:15px; margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Calibri,sans-serif" size="2" color="#7F7F7F"><span style="font-size:9pt">Emily Braker</span></font></span></font></div>
<div style="color:rgb(33,33,33); font-size:15px; margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Calibri,sans-serif" size="2" color="#7F7F7F"><span style="font-size:9pt">Vertebrate Collections Manager, Zoology
Section</span></font></span></font></div>
<div style="color:rgb(33,33,33); font-size:15px; margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Calibri,sans-serif" size="2" color="#7F7F7F"><span style="font-size:9pt">University of Colorado Museum of Natural
History</span></font></span></font></div>
<div style="color:rgb(33,33,33); font-size:15px; margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Calibri,sans-serif" size="2" color="#7F7F7F"><span style="font-size:9pt">265 UCB, Bruce Curtis Building</span></font></span></font></div>
<div style="color:rgb(33,33,33); font-size:15px; margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Calibri,sans-serif" size="2" color="#7F7F7F"><span style="font-size:9pt">Boulder, CO 80309-0218</span></font></span></font></div>
<div style="color:rgb(33,33,33); font-size:15px; margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Calibri,sans-serif" size="2" color="#7F7F7F"><span style="font-size:9pt">Phone: 303-492-8466</span></font></span></font></div>
<div style="color:rgb(33,33,33); font-size:15px; margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><a href="http://www.colorado.edu/cumuseum/research-collections/vertebrates" target="_blank"><font face="Calibri,sans-serif" size="2"><span style="font-size:9pt"><font color="#0563C1">http://www.colorado.edu/cumuseum/research-collections/vertebrates</font></span></font></a></span></font></div>
<span id="ms-rterangepaste-end"></span><br>
<p style="margin-top: 0px; margin-bottom: 0px;"></p>
</div>
</div>
</div>
</div>
<div id="appendonsend"></div>
<hr style="display:inline-block;width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> on behalf of Lennart Lennuk <Lennart.Lennuk@loodusmuuseum.ee><br>
<b>Sent:</b> Thursday, September 30, 2021 5:36 AM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu <nhcoll-l@mailman.yale.edu><br>
<b>Subject:</b> [Nhcoll-l] collecting and preserving animal tissue samples</font>
<div> </div>
</div>
<style>
<!--
@font-face
{font-family:Wingdings}
@font-face
{font-family:"Cambria Math"}
@font-face
{font-family:Calibri}
p.x_MsoNormal, li.x_MsoNormal, div.x_MsoNormal
{margin:0cm;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri",sans-serif}
a:link, span.x_MsoHyperlink
{color:#0563C1;
text-decoration:underline}
a:visited, span.x_MsoHyperlinkFollowed
{color:#954F72;
text-decoration:underline}
p.x_MsoListParagraph, li.x_MsoListParagraph, div.x_MsoListParagraph
{margin-top:0cm;
margin-right:0cm;
margin-bottom:0cm;
margin-left:36.0pt;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri",sans-serif}
span.x_Meililaad17
{font-family:"Calibri",sans-serif;
color:black;
font-weight:normal;
font-style:normal}
.x_MsoChpDefault
{font-family:"Calibri",sans-serif}
@page WordSection1
{margin:70.85pt 70.85pt 70.85pt 70.85pt}
div.x_WordSection1
{}
ol
{margin-bottom:0cm}
ul
{margin-bottom:0cm}
-->
</style>
<div lang="ET" link="#0563C1" vlink="#954F72">
<div class="x_WordSection1">
<p class="x_MsoNormal"><span style="color:black">Dear all,</span></p>
<p class="x_MsoNormal"><span style="color:black"> </span></p>
<p class="x_MsoNormal"><span style="color:black">Do you have good material about collecting and preserving animal tissues?</span></p>
<p class="x_MsoNormal"><span style="color:black">I already search older nh-coll list letters and spnhc wiki.</span></p>
<p class="x_MsoNormal"><span style="color:black"> </span></p>
<p class="x_MsoNormal"><span style="color:black">I have some specific questions:</span></p>
<p class="x_MsoListParagraph" style="text-indent:-18.0pt"><span style="color:black"><span style="mso-list:Ignore">-<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="color:black">what vials are best for preserving the tissue samples?</span></p>
<p class="x_MsoListParagraph" style="text-indent:-18.0pt"><span style="color:black"><span style="mso-list:Ignore">-<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="color:black">does 96 EtOH in -25 Celsius work? No money for -80 freezer</span></p>
<p class="x_MsoListParagraph" style="text-indent:-18.0pt"><span style="color:black"><span style="mso-list:Ignore">-<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="color:black">How many tissues per animal is optimal?
</span></p>
<p class="x_MsoNormal"><span style="color:black"> </span></p>
<p class="x_MsoNormal"><span style="color:black">Our purose will be to preserve the tissue samples of the animals we make for taxidermy collections in case a researcher</span></p>
<p class="x_MsoNormal"><span style="color:black">wants to have a DNA sample of the animal.</span></p>
<p class="x_MsoNormal"><span style="color:black"> </span></p>
<p class="x_MsoNormal"><span style="color:black">Best regards!</span></p>
<p class="x_MsoNormal"><span style="color:black">Lennart Lennuk</span></p>
<p class="x_MsoNormal"><span style="color:black">Head of collections</span></p>
<p class="x_MsoNormal"><span style="color:black">Estonian Museum of Natural History</span></p>
<p class="x_MsoNormal"><span style="color:black">+372 6603404, 56569916</span></p>
<p class="x_MsoNormal"> </p>
</div>
</div>
</body>
</html>