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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black;mso-fareast-language:EN-US">Thank you all for good material and advice!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black;mso-fareast-language:EN-US">I have one more question:<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black;mso-fareast-language:EN-US">How much blood is considered enough for DNA sample?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Best!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Lennart<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> Emily M. Braker [mailto:emily.braker@colorado.edu]
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<b>Sent:</b> Thursday, September 30, 2021 4:46 PM<br>
<b>To:</b> Lennart Lennuk <Lennart.Lennuk@loodusmuuseum.ee>; nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: collecting and preserving animal tissue samples<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Greetings Lennart,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">I recommend taking a look at the SPNHC wiki for guidance on collecting and storing tissue samples:<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><a href="https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection" id="LPlnk641881">https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><a href="https://spnhc.biowikifarm.net/wiki/Genetic_Resources" id="LPlnk959084">https://spnhc.biowikifarm.net/wiki/Genetic_Resources</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">But in short, we use 2ml externally threaded cryovials for tissue samples (many brands available from lab suppliers). Using 95% ETOH is an effective preservative and can be stored
in a regular freezer if you don't have access to ultracold freezing or liquid nitrogen. Volume of tissue harvested is up to your institution based on anticipated usage, space, and species rarity. We tend to take three vials (heart, muscle, liver), but many
institutions combine these samples into one vial, while others may sample additional organs. If we receive a rare or endangered species, we tend to increase our sampling.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Best,<br>
Emily<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#7F7F7F">Emily Braker</span><span style="font-size:11.5pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#7F7F7F">Vertebrate Collections Manager, Zoology Section</span><span style="font-size:11.5pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#7F7F7F">University of Colorado Museum of Natural History</span><span style="font-size:11.5pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#7F7F7F">265 UCB, Bruce Curtis Building</span><span style="font-size:11.5pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#7F7F7F">Boulder, CO 80309-0218</span><span style="font-size:11.5pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#7F7F7F">Phone: 303-492-8466</span><span style="font-size:11.5pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:#212121"><a href="http://www.colorado.edu/cumuseum/research-collections/vertebrates" target="_blank"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif">http://www.colorado.edu/cumuseum/research-collections/vertebrates</span></a></span><span style="font-size:11.5pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a>>
on behalf of Lennart Lennuk <<a href="mailto:Lennart.Lennuk@loodusmuuseum.ee">Lennart.Lennuk@loodusmuuseum.ee</a>><br>
<b>Sent:</b> Thursday, September 30, 2021 5:36 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a> <<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a>><br>
<b>Subject:</b> [Nhcoll-l] collecting and preserving animal tissue samples</span>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="xmsonormal"><span style="color:black">Dear all,</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Do you have good material about collecting and preserving animal tissues?</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">I already search older nh-coll list letters and spnhc wiki.</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">I have some specific questions:</span><o:p></o:p></p>
<p class="xmsolistparagraph" style="text-indent:-18.0pt"><span style="color:black">-</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif;color:black">
</span><span style="color:black">what vials are best for preserving the tissue samples?</span><o:p></o:p></p>
<p class="xmsolistparagraph" style="text-indent:-18.0pt"><span style="color:black">-</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif;color:black">
</span><span style="color:black">does 96 EtOH in -25 Celsius work? No money for -80 freezer</span><o:p></o:p></p>
<p class="xmsolistparagraph" style="text-indent:-18.0pt"><span style="color:black">-</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif;color:black">
</span><span style="color:black">How many tissues per animal is optimal? </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Our purose will be to preserve the tissue samples of the animals we make for taxidermy collections in case a researcher</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">wants to have a DNA sample of the animal.</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Best regards!</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Lennart Lennuk</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Head of collections</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">Estonian Museum of Natural History</span><o:p></o:p></p>
<p class="xmsonormal"><span style="color:black">+372 6603404, 56569916</span><o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
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