<meta http-equiv="Content-Type" content="text/html; charset=utf-8"><div dir="ltr">Hello Lennart,<div><br></div><div>That would probably depend on what type of genetic/genomic analysis you plan on conducting with it, as well as the taxon you are working with. You may also want to think about the IACUC protocols for the species, as blood draw amounts can be dictated by size of the animal and experimental protocol. </div><div><br></div><div>Birds have nucleated blood cells, so I have gotten DNA extractions from very small draws of blood. </div><div><br></div><div>cheers,</div><div>Beth Wommack</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Oct 14, 2021 at 3:07 AM Lennart Lennuk <<a href="mailto:Lennart.Lennuk@loodusmuuseum.ee">Lennart.Lennuk@loodusmuuseum.ee</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div lang="ET">
<div style="border:2.25pt solid red;padding:1pt 4pt">
<p class="MsoNormal" style="line-height:11.35pt">◆ This message was sent from a non-UWYO address. Please exercise caution when clicking links or opening attachments from external sources.</p>
</div>
<br>
<div>
<div class="gmail-m_-5006472803119201694WordSection1">
<p class="MsoNormal"><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black">Thank you all for good material and advice!<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black">I have one more question:<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black">How much blood is considered enough for DNA sample?<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
<div>
<p class="MsoNormal"><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black">Best!<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black">Lennart<u></u><u></u></span></p>
</div>
<p class="MsoNormal"><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
<div>
<div style="border-right:none;border-bottom:none;border-left:none;border-top:1pt solid rgb(225,225,225);padding:3pt 0cm 0cm">
<p class="MsoNormal"><b><span style="font-size:11pt;font-family:Calibri,sans-serif">From:</span></b><span style="font-size:11pt;font-family:Calibri,sans-serif"> Emily M. Braker [mailto:<a href="mailto:emily.braker@colorado.edu" target="_blank">emily.braker@colorado.edu</a>]
<br>
<b>Sent:</b> Thursday, September 30, 2021 4:46 PM<br>
<b>To:</b> Lennart Lennuk <<a href="mailto:Lennart.Lennuk@loodusmuuseum.ee" target="_blank">Lennart.Lennuk@loodusmuuseum.ee</a>>; <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> Re: collecting and preserving animal tissue samples<u></u><u></u></span></p>
</div>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black">Greetings Lennart,<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black">I recommend taking a look at the SPNHC wiki for guidance on collecting and storing tissue samples:<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><a href="https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection" id="gmail-m_-5006472803119201694LPlnk641881" target="_blank">https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection</a><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><a href="https://spnhc.biowikifarm.net/wiki/Genetic_Resources" id="gmail-m_-5006472803119201694LPlnk959084" target="_blank">https://spnhc.biowikifarm.net/wiki/Genetic_Resources</a><u></u><u></u></span></p>
</div>
<div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black">But in short, we use 2ml externally threaded cryovials for tissue samples (many brands available from lab suppliers). Using 95% ETOH is an effective preservative and can be stored
in a regular freezer if you don't have access to ultracold freezing or liquid nitrogen. Volume of tissue harvested is up to your institution based on anticipated usage, space, and species rarity. We tend to take three vials (heart, muscle, liver), but many
institutions combine these samples into one vial, while others may sample additional organs. If we receive a rare or endangered species, we tend to increase our sampling.<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black">Best,<br>
Emily<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
</div>
<div id="gmail-m_-5006472803119201694Signature">
<div>
<div id="gmail-m_-5006472803119201694divtagdefaultwrapper">
<div>
<p class="MsoNormal"><span style="font-size:9pt;font-family:Calibri,sans-serif;color:rgb(127,127,127)">Emily Braker</span><span style="font-size:11.5pt;font-family:Calibri,sans-serif;color:rgb(33,33,33)"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9pt;font-family:Calibri,sans-serif;color:rgb(127,127,127)">Vertebrate Collections Manager, Zoology Section</span><span style="font-size:11.5pt;font-family:Calibri,sans-serif;color:rgb(33,33,33)"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9pt;font-family:Calibri,sans-serif;color:rgb(127,127,127)">University of Colorado Museum of Natural History</span><span style="font-size:11.5pt;font-family:Calibri,sans-serif;color:rgb(33,33,33)"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9pt;font-family:Calibri,sans-serif;color:rgb(127,127,127)">265 UCB, Bruce Curtis Building</span><span style="font-size:11.5pt;font-family:Calibri,sans-serif;color:rgb(33,33,33)"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9pt;font-family:Calibri,sans-serif;color:rgb(127,127,127)">Boulder, CO 80309-0218</span><span style="font-size:11.5pt;font-family:Calibri,sans-serif;color:rgb(33,33,33)"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9pt;font-family:Calibri,sans-serif;color:rgb(127,127,127)">Phone: 303-492-8466</span><span style="font-size:11.5pt;font-family:Calibri,sans-serif;color:rgb(33,33,33)"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="color:rgb(33,33,33)"><a href="http://www.colorado.edu/cumuseum/research-collections/vertebrates" target="_blank"><span style="font-size:9pt;font-family:Calibri,sans-serif">http://www.colorado.edu/cumuseum/research-collections/vertebrates</span></a></span><span style="font-size:11.5pt;font-family:Calibri,sans-serif;color:rgb(33,33,33)"><u></u><u></u></span></p>
</div>
<p class="MsoNormal"><span style="font-family:Calibri,sans-serif;color:black"><u></u> <u></u></span></p>
</div>
</div>
</div>
</div>
<div class="MsoNormal" align="center" style="text-align:center">
<hr size="2" width="98%" align="center">
</div>
<div id="gmail-m_-5006472803119201694divRplyFwdMsg">
<p class="MsoNormal"><b><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black">From:</span></b><span style="font-size:11pt;font-family:Calibri,sans-serif;color:black"> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" target="_blank">nhcoll-l-bounces@mailman.yale.edu</a>>
on behalf of Lennart Lennuk <<a href="mailto:Lennart.Lennuk@loodusmuuseum.ee" target="_blank">Lennart.Lennuk@loodusmuuseum.ee</a>><br>
<b>Sent:</b> Thursday, September 30, 2021 5:36 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a> <<a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a>><br>
<b>Subject:</b> [Nhcoll-l] collecting and preserving animal tissue samples</span>
<u></u><u></u></p>
<div>
<p class="MsoNormal"> <u></u><u></u></p>
</div>
</div>
<div>
<div>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">Dear all,</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black"> </span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">Do you have good material about collecting and preserving animal tissues?</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">I already search older nh-coll list letters and spnhc wiki.</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black"> </span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">I have some specific questions:</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsolistparagraph"><span style="color:black">-</span><span style="font-size:7pt;font-family:"Times New Roman",serif;color:black">
</span><span style="color:black">what vials are best for preserving the tissue samples?</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsolistparagraph"><span style="color:black">-</span><span style="font-size:7pt;font-family:"Times New Roman",serif;color:black">
</span><span style="color:black">does 96 EtOH in -25 Celsius work? No money for -80 freezer</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsolistparagraph"><span style="color:black">-</span><span style="font-size:7pt;font-family:"Times New Roman",serif;color:black">
</span><span style="color:black">How many tissues per animal is optimal? </span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black"> </span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">Our purose will be to preserve the tissue samples of the animals we make for taxidermy collections in case a researcher</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">wants to have a DNA sample of the animal.</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black"> </span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">Best regards!</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">Lennart Lennuk</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">Head of collections</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">Estonian Museum of Natural History</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"><span style="color:black">+372 6603404, 56569916</span><u></u><u></u></p>
<p class="gmail-m_-5006472803119201694xmsonormal"> <u></u><u></u></p>
</div>
</div>
</div>
</div>
</div>
</blockquote></div><br clear="all"><div><br></div>-- <br><div dir="ltr" class="gmail_signature"><div dir="ltr"><div>Elizabeth Wommack, PhD<br>Curator and Collections Manager of Vertebrates<br>University of Wyoming Museum of Vertebrates<div>Berry Biodiversity Conservation Center<div>University of Wyoming,<br>Laramie, WY 82071<br><a href="mailto:ewommack@berkeley.edu" target="_blank">ewommack@</a><a href="http://uwyo.edu/" target="_blank">uwyo.edu</a></div></div><div><a href="http://www.uwymv.edu/" target="_blank">www.uwymv.</a>org</div></div><div><a href="http://www.uwymv.org/index.php/download_file/view/43/143/" target="_blank">UWYMV Collection Use Policy</a></div><div><img src="https://docs.google.com/uc?export=download&id=1S-cbOrxn8pFjBwESjZI6N8037tnknEpd&revid=0BxkSLxWP83ssMVJRVjF6RmQwTnZUVUM0ekhTWlFKL2xIb2RJPQ"><br></div></div></div>